法医学杂志

法医学杂志 ›› 2001, Vol. 0 ›› Issue (1): 17-18+6.

• 论文 • 上一篇    下一篇

广东汉族人群 D7S809的基因频率及其在亲子鉴定中的应用

吕德坚,刘秋玲,伍新尧   

  1. 中山医科大学法医学系!广东广州 510089),中山医科大学法医学系!广东广州 510089),中山医科大学法医学系!广东广州 510089)
  • 发布日期:2001-02-25 出版日期:2001-02-28

Cantonese population data of the Tetranucleotide repeat locus D7S809 and its application in paternity test

LU DEJIAN, ET AL.(FACULTY OF FORENSIC MEDICINE, SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES, GUANGZHOU, GUANGDONG 510089)   

  • Online:2001-02-25 Published:2001-02-28

被引次数

6

摘要： 利用 PCR和聚丙烯酰胺凝胶电泳分型技术，调查了以四核苷酸为重复单位的位点 D7S809在广东人群的群体资料。在 190个被调查的个体中，共发现 14个等位基因和 50种基因型。经计算杂合率、个人识别率和非父排除率分别为 0.8613、 0.9645和 0.7184，基因型分布符合 Hardy－ Weinberg平衡。 D7S809位点已成功地应用于 100例亲权鉴定案中。 D7S809是一个高度多态性、稳定、易于分型的位点，在法医学上极有应用价值。

关键词: 微卫星, D7S809, 群体数据, 亲权鉴定, 广东人

Abstract: The tetranucleotide repeat locus D7S809 was investigated in Cantonese population by polymerase chain reaction (PCR) amplification, subsequent polyacryramide gel electrophoresis and silver staining. 14 alleles and 50 genotypes were detected in 190 sample individuals. All alleles differed in size by 4bp repeat. No interalleles were found. The heterozygosity, power of discrimination and chance of non－ paternal exclusion were 0.8613, 0.9645 and 0.7184 respectively. No significant deviations from Hardy－ Weinberg equilibrium were found. The D7S809 was a highly polymorphic, stable and accurate type locus and had been successfully applied to 100 paternity test cases.

Key words: microsatellite, D7S809, population data, paternity test, Cantonese