法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (5): 343-348,356.DOI: 10.3969/j.issn.1004-5619.2010.05.007

• 论著 • 上一篇    下一篇

InDel_typer30：用于中国5个主要民族DNA鉴定的多重PCR系统

赵书民，张素华，李成涛   

  1. （司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063）
  • 发布日期:2010-10-25 出版日期:2010-10-28
  • 通讯作者: 通信作者：李成涛，男，副研究员，主要从事法医遗传学研究；E-mail：lichengtaohla@163.com
  • 作者简介:赵书民（1973—），男，河南开封人，博士，主要从事法医遗传学研究；E-mail：zhaoshuminxl@hotmail.com
  • 基金资助:

    中央级科研院所社会公益研究资助项目（GY0902）

InDel_typer30: A Multiplex PCR System for DNA Identification among Five Chinese Populations

ZHAO SHU-MIN, ZHANG SU-HUA, LI CHENG-TAO   

  1. （Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China）
  • Online:2010-10-25 Published:2010-10-28

摘要： 目的 采用插入/缺失（Insertion/Deletion，InDel）多态性遗传标记，建立一种可用于中国汉族、回族、维吾尔族、蒙古族、藏族5个主要民族法医DNA鉴定的多重PCR系统。 方法 采用人类基因组浏览器和dbSNP数据库筛选具有高度遗传多态性的人类常染色体InDel标记，采用Primer 3软件设计多重PCR引物，通过复合荧光标记系统建立多重PCR扩增体系，采用该体系对汉、回、维、蒙、藏5个民族进行多态性调查。 结果 成功建立了一个包含30个InDel位点和Amelogenin性别鉴定位点的复合荧光多重PCR扩增体系，命名为InDel_typer30。多态性调查显示这30个InDel位点在上述5个主要民族中均呈高度遗传多态性，平均期望杂合度分别为：0.464、0.460、0.453、0.466和0.469，平均个人识别率分别为：0.595、0.585、0.586、0.589和0.595。该系统在5个民族中的累积个人识别率（CDP）均达到0.999 999 999 996以上。 结论 InDel_typer30是一种适用于中国汉、回、维、蒙、藏5个主要民族的法医DNA鉴定系统。

关键词: 法医遗传学, 聚合酶链反应, 插入/缺失多态性, 亲权鉴定

Abstract: Objective To develop a multiplex PCR system, using insertion/deletion（InDel） polymorphism markers, for forensic DNA identification among Han, Hui, Uighur, Mongolian and Tibetan populations in China. Methods Highly polymorphic InDel markers from human autosomes were selected using the Human Genome Browser in Galaxy system and dbSNP database. Multiplex PCR primer pairs of selected InDel markers were designed using Primer 3 software. The multiplex PCR system was developed using a five fluorescence dye labeling system. Genetic polymorphisms of selected InDel markers were investigated using the multiplex PCR system among five populations in China. Results A new multiplex genotyping system, named InDel_typer30, was successfully developed and validated in this study. The InDel_typer30 system consisted of 30 highly polymorphic InDel markers and 1 Amelogenin gender marker. The average expected heterozygosity of the 30 InDel markers was 0.464, 0.460, 0.453, 0.466 and 0.469 for the Han, Hui, Uighur, Mongolian and Tibetan populations, respectively. The average discrimination power was 0.595, 0.585, 0.586, 0.589 and 0.595 for the Han, Hui, Uighur, Mongolian and Tibetan populations, respectively. The cumulative discrimination power（CDP） were all above 0.999 999 999 996 for the 5 populations. Conclusion InDel_typer30 was a useful forensic DNA identification tool for human identification among Han, Hui, Uighur, Mongolian and Tibetan populations in China.

Key words: forensic genetics, polymerase chain reaction, insertion/deletion polymorphism, parentage testing

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