法医学杂志

法医学杂志 ›› 2001, Vol. 0 ›› Issue (1): 15-16+1.

• 论文 • 上一篇    下一篇

成都地区汉族人群D2S441位点的遗传多态性研究

黄代新,张林,吴梅筠,陈国弟,陈于波   

  1. 华西医科大学法医物证教研室!四川成都 610041),华西医科大学法医物证教研室!四川成都 610041),华西医科大学法医物证教研室!四川成都 610041),华西医科大学法医物证教研室!四川成都 610041),华西医科大学法医物证教研室!四川成都 610041)
  • 发布日期:2001-02-25 出版日期:2001-02-28

A study of genetic polymorphism of the STR locus D2S441 in Chinese Han population in Chengdu

HUANG DAIXIN, ET AL.(DEPARTMENT OF FORENSIC BIOLOGY, WEST CHINA UNIVERSITY OF MEDICAL SCIENCES, CHENGDU,SICHUAN 610041)   

  • Online:2001-02-25 Published:2001-02-28

被引次数

3

摘要： 为研究 STR位点 D2S441的遗传多态性，为法医学应用提供基础数据，应用 PCR及 PAG电泳技术对 260名成都地区汉族无关个体进行了调查，共检出 9个等位基因及 26种基因型，首次获得汉族群体频率分布 ,其等位基因片段大小范围为 131～ 155bp。该位点基因型频率分布符合 Hardy－ Weinberg平衡。家系调查证实了等位基因的传递遵循孟德尔遗传规律。其个人识别能力（ Dp）、杂合度（ H）、多态性信息含量（ PIC）和非父排除率（ PE）分别为 0.9084、 0.7885、 0.7390和 0.5778，表明该位点在法医学个人识别及亲子鉴定中具有较高的实用价值。

关键词: 短串联重复序列, 聚合酶链反应, D2S441位点, 多态性

Abstract: Using PCR and PAG, followed by silver staining, the tetrameric STR D2S441 locus was studied in 260 unrelated Chinese individuals living in Chengdu. 9 alleles and 26 genotypes were observed. The range of fragment size was 131bp to 155bp. The genotype distribution of D2S441 locus in Han population was in accordance with Hardy－ Weinberg equilibrium. Family survey confirmed Mendelian inheritance of alleles. The discriminating power (Dp), observed heterozygosity (H), polymorphism information content (PIC) and power of exclusion (PE) were 0.9084, 0.7885, 0.7390 and 0.5778 respectively. The results demonstrated that this locus was highly polymorphic and could be used for forensic identification and paternity testing.

Key words: short tandem repeat, Polymerase chain reaction, D2S441 locus, Polymorphism