法医学杂志

法医学杂志 ›› 2003, Vol. 0 ›› Issue (4): 196-198+.

• 论文 • 上一篇    下一篇

7个Y-STR基因座单倍型及其法医学应用

刘秋玲,吕德坚,陈丽娴,童大跃,陆惠玲,伍新尧   

  1. 中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室,中山大学中山医学院法医物证教研室 广东广州510089 ,广东广州510089 ,广东广州510089 ,广东广州510089 ,广东广州510089 ,广东广州510089
  • 发布日期:2003-08-25 出版日期:2003-08-28

Seven Y-specific loci haplotypes and their usefulness in forensic casework

LIU QIU-LING,LU DE-JIAN,CHEN LI-XIAN,ET AL.(FAULTY OF FORENSIC MEDICINE,ZHONG SHAN MEDICAL COLLEGE,ZHONG SHAN UNIVERSITY,GUANGZHOU510089,CHINA)   

  • Online:2003-08-25 Published:2003-08-28

摘要： 目的调查7个Y-STR基因座单倍型及其法医学应用。方法利用二组复合扩增体系,(Ⅰ:DYS391、GATA-A4、GATA-A10和GATA-H4;Ⅱ:DYS439、DYS437和DYS434)检测7个Y染色体特异性的STR基因座,并用聚丙烯酰胺凝胶电泳和银染显色技术进行基因分型。结果在广东汉族372名无关男性个体中,二组7个基因座分别检出5、7、6、5和6、4、4个等位基因,共检出254种单倍型,其中201种为唯一的。单倍型基因多样性为0.9960。结论7个Y-STR基因座具有很高的识别能力,对建立Y染色体STR数据库,研究群体遗传学和进行法医学鉴定有重要意义。

关键词: Y-STR, 单倍型, 复合扩增

Abstract: Objective To study genetic polymorphism of7Y-specific short tandem repeats(STR)and as-sess their usefulness in forensic casework.Methods7Y-STR have been amplified in two multiplex reac-tions,(Multiplex I:DYS391,GATA-A4,GATA-A10and GATA-H4.Multiplex II:DYS439,DYS437and DYS434).PCR products were separated by polyacrylamide gels electrophoresis followed by silver stain.Results When372unrelated individuals from the Han population in Guangdong were detected by those system,DYS391,GATA-A4,GATA-A10,GATA-H4,DYS439,DYS437and DYS434showed5,7,6,5,6,4,4al-leles,respectively.A total of254different haplotypes were identified,of which201(79.13%)were found in single individuals.The overall haplotypes diversity reached0.9960.Conclusion The7Y-STR loci are highly genetic polymorphism and they will be very powerful for establishing Y-STR database,understand-ing human origin,paternity testing and personal identification.

Key words: Y-STR, haplotypes, multiplex, PCR