法医学杂志

法医学杂志 ›› 2004, Vol. 0 ›› Issue (1): 9-12+7.

• 论文 • 上一篇    下一篇

大鼠不同程度脑挫伤后caspase-3表达的研究

陶陆阳,陈溪萍,卞士中,丁梅,官大威,王继红   

  1. 苏州大学医学院法医学教研室,苏州大学医学院法医学教研室,苏州大学医学院法医学教研室,中国医科大学法医学院,中国医科大学法医学院,中国人民解放军第二0二医院病理科 江苏苏州215007 ,江苏苏州215007 ,江苏苏州215007 ,辽宁沈阳110001 ,辽宁沈阳110001 ,辽宁沈阳110001
  • 发布日期:2004-02-25 出版日期:2004-02-28

The study on the expression of caspase-3 in experimental brain contusion in rats

TAO LU-YANG,CHEN XI-PING,BIAN SHI-ZHONG,ET AL.(SCHOOL OF MEDICINE,SUZHOU UNIVERSITY,SUZHOU215007,CHINA)   

  • Online:2004-02-25 Published:2004-02-28

摘要： 目的阐明不同程度及不同时间脑挫伤后caspase-3表达的变化。方法应用免疫组织化学染色、Westernblot和RT-PCR方法对48例实验性脑挫伤大鼠的脑组织进行了检验,同时以3例非脑挫伤和3例假手术的脑组织做对照。结果伤后1hcaspase-3即有表达,24～48h达高峰,挫伤后14dcaspase-3仍维持较高水平。阳性细胞多集中于挫伤区及周围带皮质和脑实质内海马区,不同程度脑挫伤各组之间阳性细胞面积和灰度存在差异;损伤组caspase-3酶原裂解增加,不同程度脑挫伤各组之间存在一定的差异;损伤组cas鄄pase-3表达量明显高于对照组;不同程度脑挫伤组之间于各个时间段caspase-3mRNA表达未见差异。结论Caspase-3表达增加,可辅助诊断1h～14d的脑挫伤,caspase-3阳性细胞面积和灰度的变化及酶原裂解程度与脑挫伤程度有关。

关键词: 脑挫伤, caspase-3, 脑挫伤形成时间, 脑挫伤程度, 凋亡

Abstract: Objective To investigate the expression of caspase-3in different posttraumati c intervals and severity of brain injury.Methods The study examined brain tissue samples of slight(n=24),severe(n=24)brain injury and control(n=6)of rat,using i mmunohistochemical staining,western-blot and RT-PCR method.Results Up-regulating of caspase-3expression was found in tissue from traumatic brain injury compared with controls in early1hour after TBI,and lasted for14days.The gray degree and threshold area of caspase-3positive cells is different in different severity of brain injury.Conclusion The increasing of caspase-3expression indicates that TBI exists.The gray degree,threshold area of caspase-3positive cells and the cleava ge degree of pro-caspase-3have association with the severity of brain injury.

Key words: cerebral contusion, casapse-3, post contusion intervals, severity, apoptosis