法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (2): 88-92.DOI: 10.3969/j.issn.1004-5619.2015.02.003

• 论著 • 上一篇    下一篇

大鼠双后肢挤压伤后肺、肝细胞的凋亡

赵  杰1，王华荣2，步建衡1，左  敏3，张国忠3   

  1. （1. 河北省公安厅物证鉴定中心，河北 石家庄 050000； 2. 沧州市公安局刑事科学技术研究所，河北 沧州 061000； 3. 河北医科大学法医学系，河北 石家庄 050051）
  • 发布日期:2015-04-25 出版日期:2015-04-28
  • 作者简介:赵杰（1983—），男，山西介休人，硕士，主要从事法医病理学工作和研究；E-mail：zhaojie142433@sina.com

Apoptosis in Lungs and Liver after Crush Injury of Hindlimbs in Rat

（1. CENTER OF EVIDENCE IDENTIFICATION, PUBLIC SECURITY DEPARTMENT OF HEBEI PROVINCE, SHIJIAZHUANG 050000, CHINA; 2. CRIMINAL INVESTIGATION TEAM, CANGZHOU PUBLIC SECURITY BUREAU, CANGZHOU 061000, CHINA; 3. SCHOOL OF FORENSIC MEDICINE, HEBEI MEDICAL UNIVERSITY, SHIJIAZHUANG 050051, CHINA）   

  1. ZHAO Jie1, WANG Hua-rong2, BU Jian-heng1, ZUO Min3, ZHANG Guo-zhong3
  • Online:2015-04-25 Published:2015-04-28

摘要： 目的 研究大鼠双后肢挤压伤后肺、肝细胞的凋亡过程，探讨挤压伤损伤机制。 方法 建立大鼠双后肢挤压伤模型，采用TUNEL法对大鼠肺、肝细胞凋亡进行检测，免疫组织化学法检测凋亡相关蛋白Bax、Bcl-2、caspase-3的表达。 结果 与对照组相比，损伤组大鼠双后肢局部肌肉组织明显损伤，肺、肝细胞凋亡明显增多（P<0.05），凋亡相关蛋白Bax上调、Bcl-2下调、caspase-3被激活（P<0.05）。 结论 大鼠双后肢挤压伤后引起肺、肝细胞凋亡明显增多，可能是损伤释放的相关因子介导了细胞凋亡的发生。

关键词: 法医病理学, 创伤和损伤, 挤压, 细胞凋亡, Bax, Bcl-2, caspase-3, 大鼠

Abstract: Objective To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanism of crush injury. Methods The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry. Results Compared with the control group, the partial muscle injury of rat’s hindlimbs was more serious with more apoptosis observed in lungs and liver （P<0.05）. The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated （P<0.05）. Conclusion The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The correlation factor released during tissue injury may mediate apoptosis process.

Key words: forensic pathology, wounds and injuries, crush, apoptosis, Bax, Bcl-2, caspase-3, rats

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