法医学杂志 ›› 2006, Vol. 0 ›› Issue (4): 277-280.

• 论文 • 上一篇    下一篇

云南怒族X染色体5个STR基因座遗传多态性

高树辉;乔可;李生斌;   

  1. 西安交通大学医学院法医系,西安交通大学医学院法医系,西安交通大学医学院法医系 陕西西安710061中国人民公安大学刑事科学技术系,北京100038,陕西西安710061,陕西西安710061
  • 发布日期:2006-08-25 出版日期:2006-08-28

Genetic Polymorphisms of 5 X-STR Loci in Yunnan Nu Population

GAO SHU-HUI1,2, QIAO KE1, LI SHENG-BIN1 (1.THE NATIONAL LABORATORY OF FORENSIC SCIENCE, COLLEGE OF MEDICINE, XI′AN JIAOTONG UNIVERSITY, XI′AN 710061,CHINA; 2.DEPARTMENT OF FORENSIC SCIENCE, CHINESE PEOPLE′S PUBLIC SECURITY UNIVERSITY,BEIJING 100038,CHINA)   

  • Online:2006-08-25 Published:2006-08-28

摘要: 目的以云南怒族无关个体为研究对象,调查怒族群体DXS6804、DXS6799、DXS8378、DXS7130、DXS7132基因座的基因频率及基因型频率分布,建立群体遗传数据库。方法用聚合酶链反应(PCR)、变性聚丙烯酰胺凝胶电泳结合银染的方法,检测100名云南怒族个体X染色体上5个STR基因座的重复序列长度变化。结果云南怒族群体5个STR基因座具有遗传多态性,χ2检验表明多态性分布符合Hardy-Weinberg平衡定律。结论云南怒族群体DXS6804、DXS6799、DXS8378、DXS7130和DXS7132基因座,可用于法医学个体识别、亲子鉴定等研究。

关键词: 云南怒族, X染色体, STR, 遗传多态性

Abstract: Objective To investigate the allele and genotype frequencies of DXS6804,DXS6799,DXS8378,DXS7130 and DXS7132 in unrelated individuals of Nu population and establish the related genetic database. Methods Five X-STR loci were analyzed by PCR followed PAGE and silver staining. Results The allele frequencies of the five X-STRs in Yunnan Nu population are in accordance with Hardy-Weinberg equilibrium. Conclusion Five X-STRs loci of Nu population could be used in forensic identification.

Key words: Nu population, X-chromosome, short tandem repeat(STR), genetic polymorphism