法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (4): 302-303.

• 论文 • 上一篇    下一篇

吸附性载体上微量血痕的DNA分型

陈荣华;宋清;徐庆文;董研;   

  1. 上海市刑事科学技术研究所,上海市公安局嘉定分局,上海市刑事科学技术研究所,上海市刑事科学技术研究所 上海200083,上海201800,上海200083,上海200083
  • 发布日期:2007-08-25 出版日期:2007-08-28

DNA Genetyping of the Trace Bloodstains on the Adsorbent Object

CHEN RONG-HUA1,SONG QING2,XU QIN-WEN1,DONG YAN1(1.SHANGHAI INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY,SHANGHAI 200083,CHINA;2.JIADING BRANCH OF SHANGHAI PUBLIC SECURITY BUREAU,SHANGHAI 201800,CHINA)   

  • Online:2007-08-25 Published:2007-08-28

被引次数

9

摘要： 目的研究吸附性载体上微量血痕的DNA提取及其检验。方法用Chelex-100法、QIAamp MiniKit、及QIAamp Mini Kit改良法提取吸附性载体上微量血痕中的DNA,进行PCR扩增及STR检验。结果采用Chelex-100法及QIAamp Mini Kit的分型成功率很低;采用QIAamp Mini Kit的改良法能较好的得到分型图谱。结论采用QIAamp Mini Kit的改良法能较好的提取吸附性载体上微量血痕的模板DNA。

关键词: DNA分型, 吸附性载体, 微量血痕, QIAamp Mini Kit, Chelex-100

Abstract: Objective To study the extraction and analysis of DNA from trace bloodstains on the adsorbent object.Methods DNA was extracted by Chelex-100,QIAamp Mini Kit,and modified QIAamp Mini Kit,respectively.The extracted DNA was amplified and short tandem repeat(STR) loci were genotyped by multiplex PCR procedures,and then analyzed by ABI 3100 Genetic Analyzer.Results The STR loci could be better genotyped with modified QIAamp Mini Kit,compared to poorer genotyping results obtained with Chelex-100 extraction method and QIAamp Mini Kit.Conclusion Our data indicate that DNA extracted from trace bloodstains on adsorbent object with modified QIAamp Mini Kit serves a better DNA template for amplification and genotyping.

Key words: DNA typing, adsorbent object, trace bloodstains, QIAamp Mini Kit, modified, Chelex-100