法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (4): 304-306.

• 论文 • 上一篇    下一篇

MiniFiler试剂盒运用于法医微量物证的检验

唐建平;吴丹;张晨;周怀谷;   

  1. 上海市公安局物证鉴定中心,上海市公安局物证鉴定中心,上海市公安局物证鉴定中心,上海市公安局物证鉴定中心 上海200083,上海200083,上海200083,上海200083
  • 发布日期:2007-08-25 出版日期:2007-08-28

Application of MiniFiler Kit in Forensic DNA Testing of Low Copy Number Template

TANG JIAN-PING,WU DAN,ZHANG CHEN,ZHOU HUAI-GU (INSTITUTE OF FORENSIC SCIENCE,SHANGHAI MUNICIPAL PUBLIC SECURITY BUREAU,SHANGHAI 200083,CHINA)   

  • Online:2007-08-25 Published:2007-08-28

被引次数

20

摘要： 目的应用MiniFiler试剂盒对法医微量物证进行DNA分析。方法采用MiniFiler试剂盒对样本进行DNA分型,确定样本9个STR基因座的等位基因型。结果10pg DNA模板经MiniFiler试剂盒扩增后,能够进行DNA分型,但40pg以上的DNA方可得到稳定可靠的分型结果。结论MiniFiler试剂盒可以用于法医微量物证的STR分析。

关键词: MiniFiler试剂盒, 微量物证, 法医DNA分析

Abstract: Objective To detect low copy number of DNA samples by using a newly launched commercial miniSTR detection kit(MiniFiler) in forensic practice.Methods Low concentration and/or challenged forensic DNA samples were analyzed according to protocols provided by the manufacturer(Applied Biosystems,Foster City,USA).Results DNA samples as low as 10 pg could be amplified by MiniFiler kit,and the optimal DNA quantity was 40 pg or above.Conclusion MiniFiler kit can be used for analysis of low copy number STR.

Key words: miniFiler kit, challenged DNA samples, forensic DNA analysis