法医学杂志

法医学杂志 ›› 2007, Vol. 23 ›› Issue (5): 349-352.

• 论文 • 上一篇    下一篇

LC-MS/MS-MRM筛选血液中22种有毒生物碱成分

刘伟;沈敏;沈保华;向平;吴何坚;   

  1. 复旦大学上海医学院法医学系,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所 上海200032 司法部司法鉴定科学技术研究所,上海200063,上海200063,上海200063,上海200063,上海200063
  • 发布日期:2007-10-25 出版日期:2007-10-28

Simultaneous Screening for 22 Poisonous Alkaloids in Blood by Liquid Chromatography-Tandem Mass Spectrometry with Multiple-Reaction Monitoring

LIU WEI1,2,SHEN MIN2,SHEN BAO-HUA2,XIANG PING2,WU HE-JIAN2 (1.DEPARTMENT OF FORENSIC MEDICINE,SHANGHAI MEDICAL COLLEGE,FUDAN UNIVERSITY,SHANGHAI 200032,CHINA; 2. INSTITUTE OF FORENSIC SCIENCES ,MINISTRY OF JUSTICE,P. R. CHINA,SHANGHAI 200063,CHINA)   

  • Online:2007-10-25 Published:2007-10-28

被引次数

45

摘要： 目的建立液相色谱-串联质谱法对血液中的22种常见有毒生物碱成分进行筛选分析。方法血液以丁丙诺菲为内标经液液提取后,用液相色谱-串联质谱仪以电喷雾电离(ESI+)、多反应监测(MRM)方式进行分析。结果以化合物的保留时间、两对母离子/子离子对定性,最低检出限为0.1～20ng/mL。结论该方法选择性佳、灵敏度高,适用于法医毒物分析和临床毒物分析中有毒生物碱的分析。

关键词: 有毒生物碱, 筛选, 液相色谱-串联质谱法, 多反应检测

Abstract: Objective To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening for 22 poisonous alkaloids in blood. Methods This method involves a liquid-liquid extraction (LLE) followed by liquid chromatography-tandem mass spectrometry with multiple-reaction monitoring(MRM). After blood was extracted with buprenorphine as the internal standard,the target compounds were analyzed with LC-MS/MS-ESI in the positive ionization mode. Results Identification was based on the compound′s retention time and two precursor-to-product ion transitions. The limits of detection ranged from 0.1ng/mL to 20 ng/mL in blood. Conclusion The method was sufficiently selective and sensitive to detect poisonous alkaloids and can be applied in forensic and clinical toxicology.

Key words: poisonous alkaloids, screening, LC-MS/MS, multiple-reaction monitoring(MRM)