法医学杂志

法医学杂志 ›› 2021, Vol. 37 ›› Issue (4): 516-523.DOI: 10.12116/j.issn.1004-5619.2021.310205

所属专题： 新精神活性物质

• 专题 • 上一篇    下一篇

QuEChERS结合UPLC-MS/MS检测血液中3种色胺类新精神活性物质

侯伟1，2， 王燕燕1， 张瑛1， 张蕾萍3， 辛国斌1， 覃仕扬1， 王继芬2   

  1. 1. 法庭毒物分析公安部重点实验室 北京市公安局，北京 100192； 2. 中国人民公安大学侦查学院，北京 100038； 3. 公安部物证鉴定中心，北京 100038
  • 发布日期:2021-08-25 出版日期:2021-08-28
  • 通讯作者: 王燕燕，女，博士，高级工程师，主要从事毒物毒品检验；E-mail：61083942@qq.com
  • 作者简介:侯伟（1996—），男，硕士研究生，主要从事刑事技术研究；E-mail：760466281@qq.com
  • 基金资助:
    法庭毒物分析公安部重点实验室（北京市公安局）开放基金资助项目（2019FTDWFX05）

Determination of Three Types of New Psychoactive Tryptamines in Blood by QuEChERS Combined with UPLC-MS/MS

HOU Wei1,2, WANG Yan-yan1, ZHANG Ying1, ZHANG Lei-ping3, XIN Guo-bin1, QIN Shi-yang1, WANG Ji-fen2   

  1. 1. Key Laboratory of Forensic Toxicology, Ministry of Public Security, Beijing Public Security Bureau, Beijing 100192, China; 2. School of Investigation, People’ s Public Security University of China, Beijing 100038, China; 3. Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China
  • Online:2021-08-25 Published:2021-08-28

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摘要： 目的 建立一种QuEChERS结合超高效液相色谱-串联质谱法（ultra-high performance liquid chromatography-tandem mass spectrometry，UPLC-MS/MS）快速筛查和检验人体血液中3种色胺类新精神活性物质——N，N-二烯丙基-5-甲氧基色胺（5-MeO-DALT）、N-甲基-N-异丙基-5-甲氧基色胺（5-MeO-MiPT）、N，N-二异丙基-5-甲氧基色胺（5-MeO-DiPT）的方法。 方法 分别考察了萃取剂种类、盐析剂种类及用量、吸附剂用量等条件对3种色胺类物质检验结果的影响。血液样品经QuEChERS方法处理后采用UPLC-MS/MS法进行检测。 结果 人体血液中5-MeO-DALT、5-MeO-MiPT、5-MeO-DiPT分别在0.5~100、0.5~100、0.2~100 ng/mL范围内具有良好的线性关系，相关系数均大于0.99，检出限为0.1~0.2 ng/mL，回收率范围在84.86%~94.57%，日内及日间精密度良好。 结论 该方法简单、快速、易于操作、回收率高，适用于血液中色胺类物质的定性定量分析，能够为公安机关处理相关案件提供借鉴和参考。

关键词: 法医学；毒物分析；新精神活性物质；色胺类；QuEChERS；超高效液相色谱-串联质谱法；N, N-二烯丙基-5-甲氧基色胺；N-甲基-N-异丙基-5-甲氧基色胺；N, N-二异丙基-5-甲氧基色胺；血液

Abstract: Objective To establish a method combining QuEChERS and ultra-high liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for rapid screening and testing of three types of new psychoactive tryptamines in human blood： 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT. Methods The effects of the type of extractant, the type and dosage of salting-out agent, and the dosage of adsorbent on the test results of the three tryptamines were investigated. Blood samples were processed by QuEChERS method and then determined by UPLC-MS/MS. Results The linear relationships of 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT in human blood were good in the range of 0.5-100, 0.5-100 and 0.2-100 ng/mL, respectively, with their coefficients higher than 0.99. The limits of detection （LODs） were 0.1-0.2 ng/mg. The recoveries ranged from 84.86% to 94.57%. Intra-day and inter-day precisions were good. Conclusion The method is simple, rapid, easy to operate and has a high recovery. It is suitable for the qualitative and quantitative study of tryptamines in blood and can provide the reference for public security organs to deal with related cases.

Key words: forensic medicine, toxicological analysis, new psychoactive substance, tryptamines, QuEchERS, ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）, 5-MeO-DALT, 5-MeO-MiPT, 5-MeO-DiPT, blood

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