法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (6): 425-427.

• 论文 • 上一篇    下一篇

X染色体STR荧光复合扩增体系的建立及其应用

刘秋玲;吕德坚;赵虎;   

  1. 中山大学中山医学院法医学系;
  • 发布日期:2009-12-25 出版日期:2009-12-28

Development and Application of X -chromosomal STR Multiplex Amplification System

LIU QIU-LING, L DE-JIAN, ZHAO HU (DEPARTMENT OF FORENSIC MEDICINE, ZHONGSHAN MEDICAL COLLEGE, SUN YAT-SEN UNIVERSITY, GUANGZHOU 510080, CHINA)   

  • Online:2009-12-25 Published:2009-12-28

被引次数

6

摘要： 目的研究X染色体STR在法医学亲权鉴定中的应用。方法利用荧光标记引物复合PCR技术,在同一反应管中同时检测DXS6801、DXS9902、DXS6809、DXS6803、DXS6804和DXS67996个X-STR基因座,采用3100遗传分析仪电泳和GeneMapper IDv 3.1软件进行基因分型。结果本体系同时分析6个X-STR基因座,结果清晰,灵敏度高,重复性好。结论本研究的6个X-STR基因座复合扩增体系,在法医学个体识别特别是女性的亲权鉴定中有重要应用价值。

关键词: 法医遗传学, 同胞关系, 染色体, 人, X, 荧光复合扩增

Abstract: Objective To explore the application of X-chromosomal STR(X-STR) for forensic identification and paternity testing. Methods Six X-STR loci DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and 3100 Genetic Analyzer and GeneMapper ID v3.1 Analysis Software. Results The alleles of all six X-STR loci were successfully obtained with unambiguous genotyping, high sensitivity and reproducibility. Conclusion The multiplex PCR of six X-STR loci is useful in forensic identification, particularly for sisters cases.

Key words: forensic genetics, sibling relations, chromosomes, human, X, fluorescent multiplex PCR