法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (3): 161-163,177.DOI: 10.3969/j.issn.1004-5619.2011.03.001

• 论著 •    下一篇

小鼠死后脑RNA降解与死亡时间的相关性

朱  怡1，董迎春2，梁伟波1，张  林1   

  1. （1. 四川大学 华西基础医学与法医学院物证教研室，四川 成都 610041； 2. 中国人民公安大学 刑事科学技术系，北京 100038）
  • 发布日期:2011-06-25 出版日期:2011-06-28
  • 通讯作者: 梁伟波，男，讲师，主要从事法医物证学研究；E-mail：liangweibo@scu.edu.cn;;; 张林，男，教授，主要从事法医物证学研究；E-mail：zhanglin@scu.edu.cn
  • 作者简介:朱怡（1985—），女，江苏常熟人，硕士研究生，主要从事法医物证学研究；E-mail：zhuyi0216@gmail.com
  • 基金资助:

    国家自然科学基金项目（30801317）

Relationship between RNA Degradation and Postmortem Interval in Mice

ZHU YI1, DONG YING-CHUN2, LIANG WEI-BO1, ZHANG LIN1   

  1. （1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China; 2. Department of Criminal Science and Technology, Chinese People’s Public Security University, Beijing 100038, China）
  • Online:2011-06-25 Published:2011-06-28

被引次数

11

摘要： 目的 探讨小鼠死后不同时间和温度下脑组织β-actin mRNA、18S rRNA的降解规律与死亡时间的关系。 方法 将24只健康成年C57BL/6小鼠随机分为2组，每组12只，断颈法处死后分别置于不同的环境温度中（4 ℃、37 ℃），恒定环境湿度为80％。分别于死后即刻和0.5、2、6、24、48 h取小鼠脑组织进行总RNA提取。利用RT-PCR技术和实时荧光定量PCR技术测定每一样本中β-actin mRNA和18S rRNA的Ct值，进一步计算每个样本中18S rRNA与β-actin mRNA的含量比值，对二者含量比值与死亡时间的关系进行统计学回归分析。 结果 37 ℃下，RNA降解速度明显快于4 ℃，表明温度与死后RNA的降解速度呈正相关。与β-actin mRNA的稳定性相比，18S rRNA更为稳定。 结论 通过采用实时荧光定量PCR技术研究小鼠死后脑组织中β-actin mRNA和18S rRNA的降解规律，可为法医学死亡时间推断提供新的理论基础。

关键词: 法医病理学, 聚合酶链反应, RNA, 核糖体, 18S, &beta, -actin, 脑, 死亡时间, 小鼠

Abstract: Objective To investigate the degradation changes of β-actin mRNA and 18S rRNA in different time points and temperature after death, and to explore the relationship between the changes and postmortem interval（PMI） in the brain of mice. Methods Twenty-four health adult C57BL/6 mice were randomly divided into two groups（12 each group）. They were sacrificed by cervical dislocation and placed in chamber with two different temperature（4 ℃ and 37 ℃, humidity was 80%）. The mice brains were sampled at 6 different time points（immediately, 0.5 h, 2 h, 6 h, 24 h, 48 h）, and total brain RNA were extracted. Ct value of each sample was obtained using RT-PCR and real-time PCR technology, and β-actin mRNA and 18S rRNA content ratio was calculated. The correlation between the content ratio and PMI was expressed using statistical regression analysis. Results At 37 ℃, RNA degradation rate was faster than 4 ℃, which showed that there was correlation between temperature and RNA degradation. Comparing with the stability of β-actin mRNA, 18S rRNA was more stable. Conclusion The study on degradation of β-actin mRNA and 18S rRNA in mice brain using real time PCR technology could provide a new theoretical basis for estimation of PMI and would be supplementary to the traditional methods.

Key words: forensic pathology, polymerase chain reaction, RNA, ribosomal, 18S, β-actin, brain, postmortem interval, mice

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