法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (5): 333-336.DOI: 10.3969/j.issn.1004-5619.2012.05.003

• 论著 • 上一篇    下一篇

电击损伤后大鼠CK-MB及HSP 60的水平变化

刘慧通1，付高文2，赵  泽1，丁素真1，王乔峰1，陈  蕾1，谢亚男1，王振原1   

  1. （1. 西安交通大学医学院，陕西 西安 710061； 2. 白水县公安局，陕西 白水 715600）
  • 发布日期:2012-10-25 出版日期:2012-10-28
  • 通讯作者: 王振原，男，博士，教授，博士研究生导师，主要从事法医病理学科研、教学和司法鉴定工作；E-mail：wzy218@mail.xjtu.edu.cn
  • 作者简介:刘慧通（1989—），男，山东济南人，在读本科生；E-mail：liuhuitong@126.com
  • 基金资助:

    国家大学生创新性实验计划资助项目（091069868）

Changes of CK-MB and HSP 60 in Electrical-Injuried Rats

LIU HUI-TONG1, FU GAO-WEN2, ZHAO ZE1, DING SU-ZHEN1, WANG QIAO-FENG1, CHEN LEI1, XIE YA-NAN1, WANG ZHEN-YUAN1   

  1. （1. Medical College of Xi’an Jiaotong University, Xi’an 710061, China; 2. Baishui Public Security Bureau, Baishui 715600, China）
  • Online:2012-10-25 Published:2012-10-28

摘要： 目的 观察无电流斑电击伤后大鼠血清肌酸激酶同工酶（creatine kinase-MB，CK-MB）及热休克蛋白60（heat shock protein 60，HSP 60）的变化水平，探讨其变化与电流损伤时间的相关性，评估电击后相应组织细胞损伤情况。 方法 利用110 V交流电电击大鼠，建立大鼠无电流斑电击损伤模型。取大鼠的血液进行血清CK-MB全自动生化检测，心肌和肝左叶组织进行HSP 60免疫组织化学染色。 结果 电击组大鼠血清CK-MB在电击损伤早期即升高，在30 min达到峰值，此后递减，3~5 h下降趋势减缓，于电击后6 h趋于平稳。免疫组织化学染色显示，电击后大鼠心肌和肝细胞中HSP 60表达水平产生一定的规律性改变。 结论 电流损伤后大鼠血清CK-MB和组织HSP 60水平发生一定的规律性改变，对于电击损伤的诊断和电击后内部器官损伤的评估具有辅助意义。

关键词: 法医病理学, 电击伤, 肌酸激酶, MB型, 热休克蛋白质类, 大鼠

Abstract: Objective To investigate the changes of creatine kinase-MB （CK-MB） and heat shock protein 60 （HSP 60） in rats without electric marks after electric injury, to identify the relationship of the CK-MB, HSP 60 and the time of electric injuries, and to evaluate the damage to cells after electric injury. Methods The animal model of electric injury without electric marks was established by alternating current （voltage 110 V）. Automatic biochemistry analyzer was used to detect the serum CK-MB and immunohistochemical staining technology was used to analyze the tissues of myocardium and left lobe of liver. Results The amount of serum CK-MB was increased when the rats were injuried, and reached the peak at 30 min. Then the amount of CK-MB began to decrease and showed a slight downward trend in 3-5 h after electric injury, and leveled off at 6 h. Immunohistochemistry staining also showed the changes of HSP 60 of rats’ myocardial cells and hepatic cells regularly after electric injury. Conclusion The regular changes of serum CK-MB and tissular HSP 60 in rats can be used to diagnosis electric injury and assess the injury of internal organs after the electric injury without electric marks.

Key words: forensic pathology, electric injuries, creatine kinase, MB form, heat-shock proteins, rats

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