法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (1): 26-30.DOI: 10.3969/j.issn.1004-5619.2016.01.006

• 论著 • 上一篇    下一篇

甲维盐中毒死亡小鼠体内的分布及死后再分布

唐玮玮1，林玉才1，卢延旭2   

  1. （1. 厦门市公安局刑侦支队，福建 厦门 361001； 2. 中国刑事警察学院法医学系，辽宁 沈阳 110854）
  • 发布日期:2016-02-25 出版日期:2016-02-28
  • 通讯作者: 卢延旭，男，教授，博士研究生导师，主要从事法医毒理学研究；E-mail：yanxulu4689@sina.com
  • 作者简介:唐玮玮（1981—），女，硕士研究生，主要从事法医毒理学研究；E-mail：31314622@qq.com
  • 基金资助:

    公安部科技应用创新计划项目（2010YYCXXJXY112）

Biodistribution and Postmortem Redistribution of Emamectin Benzoate in Intoxicated Mice

TANG WEI-WEI1, LIN YU-CAI1, LU YAN-XU2   

  1. （1. Criminal Investigation Detachment, Xiamen Public Security Bureau, Xiamen 361001, China; 2. Department of Forensic Medicine, China Criminal Police College, Shenyang 110854, China）
  • Online:2016-02-25 Published:2016-02-28

摘要：  目的 研究甲氨基阿维菌素苯甲酸盐（简称甲维盐）中毒死亡小鼠的致死血浓度、靶器官组织、毒物蓄积库和死后毒物再分布的特征。 方法 采用灌胃法建立中毒小鼠模型，动态观察急性中毒组、亚急性中毒组小鼠的主要中毒症状和临床死亡时间。观察中毒死后小鼠各器官组织病理形态学改变，应用酶联免疫吸附试验测定死后0、24、48、72 h甲维盐体内分布及死后再分布，采用高效液相色谱法测定中毒小鼠的致死血浓度和死后各时间点的血中甲维盐浓度。 结果 中毒小鼠均在灌胃后15～30 min内依次出现神经、呼吸系统症状。急性中毒组小鼠的临床死亡时间为（45.8±7.9） min，亚急性中毒组为（8.0±1.4） d。甲维盐的急性致死血浓度范围为447.164 0～524.463 5 mg/L。光镜及荧光显微镜下各器官组织均见明显的病理改变；小鼠中毒死后72 h内，血、心、肝、脾、肺、肾及脑甲维盐浓度变化具有规律性（P<0.05）。 结论 甲维盐中毒作用的主要靶器官为心、肝、肾、肺、脑和接触部位（胃），其主要蓄积库为肾、肝，甲维盐在小鼠体内存在死后再分布现象。

关键词: 法医病理学, 法医毒理学, 中毒, 甲氨基阿维菌素苯甲酸盐, 死后再分布, 小鼠

Abstract: Objective To investigate the lethal blood level, the target organs and tissues, the toxicant storage depots and the postmortem redistribution in mice died of emamectin benzoate poisoning. Methods The mice model of emamectin benzoate poisoning was established via intragastric injection. The main poisoning symptoms and the clinical death times of mice were observed and recorded dynamically in the acute poisoning group as well as the sub-acute poisoning death group. The pathological and histomorphological changes of organs and tissues were observed after poisoning death. The biodistribution and postmortem redistribution of emamectin benzoate in the organs and tissues of mice were assayed by the enzyme-linked immunosorbent assay （ELISA） at 0 h, 24 h, 48 h and 72 h after death. The lethal blood concentrations and the concentrations of emamectin benzoate were detected by high performance liquid chromatography （HPLC） at different time points after death. Results The symptoms of nervous and respiratory system were observed within 15-30 min after intragastric injection. The average time of death was （45.8±7.9） min in the acute poisoning group and （8.0±1.4） d in the sub-acute poisoning group, respectively. The range of acute lethal blood level was 447.164 0-524.463 5 mg/L. The pathological changes of the organs and tissues were observed via light microscope and immunofluorescence microscope. The changes of emamectin benzoate content in the blood, heart, liver, spleen, lung, kidney and brain of poisoning mice showed regularity within 72 h after death （P<0.05）. Conclusion The target organs of emamectin benzoate poisoning include heart, liver, kidney, lung, brain and contact position （stomach）. The toxicant storage depots are kidney and liver. There is emamectin benzoate postmortem redistribution in mice.

Key words: forensic pathology, forensic toxicology, poisoning, emamectin benzoate, postmortem redistribution, mice

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