法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (4): 245-249.DOI: 10.3969/j.issn.1004-5619.2016.04.002

• 论著 • 上一篇    下一篇

人体脑组织RNA表达水平与早期PMI的相关性

吕叶辉1，马开军2，李志宏1，顾  峻1，鲍建瑛1，杨智昉1，高  静1，曾  颜3，陶  丽3，陈  龙3   

  1. （1． 上海健康医学院，上海 201318； 2． 上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室，上海 200083； 3． 复旦大学基础医学院法医学系，上海 200032）
  • 发布日期:2016-08-25 出版日期:2016-08-28
  • 通讯作者: 陈龙，男，博士，主任法医师，副教授，主要从事法医病理学及法医临床学研究；E-mail：chenlong@shmu.edu.cn
  • 作者简介:陈龙，男，博士，主任法医师，副教授，主要从事法医病理学及法医临床学研究；E-mail：chenlong@shmu.edu.cn
  • 基金资助:

    国家自然科学基金面上资助项目（81373242、81671863）；上海健康医学院种子基金项目；上海高校青年教师培养资助计划
    作者简介：吕叶辉（1989—），男，博士研究生，主要从事基础医学及法医学教学、科研工作；E-mail：yeyeliushu@163.com

Correlation between RNA Expression Level and Early PMI in Human Brain Tissue

L?譈 YE-HUI1, MA KAI-JUN2, LI ZHI-HONG1, GU JUN1, BAO JIAN-YING1, YANG ZHI-FANG1, GAO JING1, ZENG YAN3, TAO LI3, CHEN LONG3   

  1. （1. Shanghai University of Medicine & Health Science, Shanghai 201318, China; 2. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 3. Department of Forensic Medicine, School of Basic Medical Science, Fudan University, Shanghai 200032, China）
  • Online:2016-08-25 Published:2016-08-28

被引次数

2

摘要： 目的 探讨人体脑组织多种RNA指标的表达水平与早期死亡时间（PMI）的相关性。 方法 选取12例已知PMI（4.3~22.5 h）的个体，提取脑组织总RNA。选取8种常用RNA指标（β-actin、GAPDH、RPS29、18S rRNA、5S rRNA、U6 snRNA、miRNA-9、miRNA-125b），通过实时荧光定量PCR技术检测其在脑组织中的表达水平。运用geNorm软件筛选早期PMI表达稳定的内参指标，并分析其表达水平与相关因素（年龄、性别、死亡原因）的关系。运用R软件将内参标准化的RNA指标代入前期研究建立的数学模型推断PMI，计算推断PMI的误差率以验证模型精确性。 结果 5S rRNA、miRNA-9和miRNA-125b表达稳定，其表达水平与年龄、性别、死亡原因无关。利用β-actin推断PMI的误差率为24.6%，GAPDH为41.0%。 结论 5S rRNA、miRNA-9和miRNA-125b在死亡早期表达稳定，适合作为人体脑组织的内参指标。β-actin表达水平与PMI相关性良好，有望成为推测早期PMI的辅助指标。

关键词: 法医病理学, RNA, 脑, 死亡时间, 实时荧光定量PCR

Abstract: Abstract： Objective To explore the correlation between the expression levels of several RNA markers in human brain tissue and early postmortem interval （PMI）. Methods Twelve individuals with known PMI （range from 4.3 to 22.5 h） were selected and total RNA was extracted from brain tissue. Eight commonly used RNA markers were chosen including β-actin, GAPDH, RPS29, 18S rRNA, 5S rRNA, U6 snRNA, miRNA-9 and miRNA-125b, and the expression levels were detected in brain tissue by real-time fluorescent quantitative PCR. The internal reference markers with stable expression in early PMI were screened using geNorm software and the relationship between its expression level and some relevant factors such as age, gender and cause of death were analyzed. RNA markers normalized by internal reference were inserted into the mathematic model established by previous research for PMI estimation using R software. Model quality was judged by the error rate calculated with estimated PMI. Results 5S rRNA, miRNA-9 and miRNA-125b showed quite stable expression and their expression levels had no relation with age, gender and cause of death. The error rate of estimated PMI using β-actin was 24.6%, while GAPDH was 41.0%. Conclusion 5S rRNA, miRNA-9 and miRNA-125b are suitable as internal reference markers of human brain tissue owing to their stable expression in early PMI. The expression level of β-actin correlates well with PMI, which can be used as an additional index for early PMI estimation.

Key words: forensic pathology, RNA, brain, postmortem interval, real-time fluorescent quantitative PCR

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