法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (5): 326-328.DOI: 10.3969/j.issn.1004-5619.2016.05.002

• 论著 • 上一篇    下一篇

基于紫外可见积分球反射光谱法鉴定血痕经历时间

闫立强，高野   

  1. （辽宁警察学院刑事技术系，辽宁 大连 116036）
  • 发布日期:2016-10-25 出版日期:2016-10-28
  • 作者简介:闫立强（1966—），女，教授，主要从事法医病理学研究；E-mail：9072147@qq.com
  • 基金资助:

    辽宁警察学院科研基金资助项目（2015Lnpckyjjxm 002）；公安部技术研究计划资助项目（2016JSYJ304）

Determination of Bloodstain Age by UV Visible Integrating Sphere Reflection Spectrum

YAN LI-QIANG, GAO YE   

  1. （Department of Technology, Liaoning Police Academy, Dalian 116036, China）
  • Online:2016-10-25 Published:2016-10-28

被引次数

2

摘要： 目的 建立一种快速鉴定血痕陈旧度的方法。 方法 在实验室条件（20 ℃、25 ℃、30 ℃）下，使用UV-2450型紫外可见分光光度计，利用反射附件ISR-240A积分球，以BaSO4标准白板做参比，间隔一定时间测定健康人耳静脉血血痕纱布的反射光谱，采集特定波长反射率R541、R577，计算R541/R577值，使用SPSS 17.0软件进行直线拟合与回归分析。 结果 一定条件下8 h内，血痕经历时间与其特定波长紫外可见反射率比值回归分析的R2均大于0.8，回归方程成立，血痕经历时间与R541/R577值具有高度相关性。 结论 所建立的方法简便、快速、无损，可靠性好，可以对实验室条件下8 h内血痕经历时间进行鉴别。

关键词: 法医病理学, 光谱分析, 血痕, 积分球

Abstract: Objective To establish a method for rapid identification of bloodstain age. Methods Under laboratory conditions （20 ℃, 25 ℃ and 30 ℃）, an integrating sphere ISR-240A was used as a reflection accessory on an UV-2450 UV-vis spectrophotometer, and a standard white board of BaSO4 was used as reference, the reflection spectrums of bloodstain from human ears’ venous blood were measured at regular intervals. The reflection radios R541 and R577 at a specific wavelength were collected and the value of R541/R577 was calculated. The linear fitting and regression analysis were done by SPSS 17.0. Results The results of regression analysis showed that R2 of the ratios of bloodstain age to UV visible reflectivity in specific wavelengths were larger than 0.8 within 8 hours and under certain circumstances. The regression equation was established. The bloodstain age had significant correlation with the value of R541/R577. Concussion The method of inspection is simple, rapid and nondestructive with a good reliability, and can be used to identify the bloodstain age within 8 hours elapsed-time standards under laboratory conditions.

Key words: forensic pathology, spectrum analysis, blood stain, integrating sphere

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