法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (1): 6-10.DOI: 10.3969/j.issn.1004-5619.2017.01.002

• 论著 • 上一篇    下一篇

金属硫蛋白1A和2A的mRNA时序性表达（英文）

范浩亮1，2，刘淑芳1，3，孙俊红1，王英元1   

  1. （1. 山西医科大学法医学院，山西 太原 030001； 2. 海南医学院法医学教研室，海南 海口 571199； 3. 重庆公安局渝中分局，重庆 400021）
  • 发布日期:2017-02-25 出版日期:2017-02-28

Time-dependent Expression of MT1A mRNA and MT2A mRNA in the Contused Skeletal Muscle of Rats

FAN HAO-LIANG1,2, LIU SHU-FANG1,3, SUN JUN-HONG1, WANG YING-YUAN1   

  1. （1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 2. Department of Forensic Medicine, Hainan Medical University, Haikou 571199, China; 3. Yuzhong Branch of Chongqing Public Security Bureau, Chongqing 400021, China）
  • Online:2017-02-25 Published:2017-02-28
  • Contact: WANG Ying-yuan, professor, major in forensic pathology; E-mail: wyy580218@163.com SUN Jun-hong, associate professor, major in forensic pathology; E-mail: sunjunhong146@163.com
  • About author:FAN Hao-liang（1989—）, postgraduate, major in forensic pathology; E-mail: fanhaoliang198931@163.com
  • Supported by:

    the National Science Foundation of China （grant No. 81571852） and the Research Project Supported by Shanxi Scholarship Council of China （grant No. 2015-052）.

摘要： 目的 研究大鼠肌肉挫伤后金属硫蛋白（metallothionein，MT）1A mRNA、MT2A mRNA的时序性表达。 方法 54只SD大鼠被分成挫伤组（挫伤后0.5、1、6、12、18、24、30和36 h组各6只）和对照组（6只）。所有总RNA均取自大鼠的骨骼肌。利用SYBR GreenⅠ法进行RT-qPCR检测大鼠挫伤骨骼肌中MT1A mRNA、MT2A mRNA的相对表达量。 结果 MT1A mRNA、MT2A mRNA在损伤后的变化趋势与损伤时间具有一定的相关性。除了挫伤后0.5 h组外，其余各组MT1A mRNA、MT2A mRNA的表达量与对照组相比差异均有统计学意义（P<0.05）。损伤后1 h、6 h、12 h、18 h MT1A mRNA和MT2A mRNA的相对表达量呈现逐渐上升的趋势，在18 h时达到了峰值，分别为对照组的（239.41±15.20）倍和（717.42±50.76）倍；损伤后24 h时两者的表达量均明显减少；损伤后30 h再次上升，随后下降。 结论 MT1A mRNA、MT2A mRNA可能对损伤时间推断有一定的意义。

关键词: 法医病理学, 肌, 挫伤, 金属硫蛋白, 损伤时间推断

Abstract: Objective To investigate the time-dependent expression of metallothionein （MT） 1A mRNA and MT2A mRNA in contused skeletal muscle of rats. Methods A total of 54 Sprague-Dawley rats were used in this study. The rats were divided into two parts: control group （n=6） and contusion groups （0.5, 1, 6, 12, 18, 24, 30, and 36 h after contusion, n=6）. Total RNA was extracted from skeletal muscle. The expression levels of MT1A mRNA and MT2A mRNA were detected by SYBR Green I real-time PCR. Results The expression trends of the two potential marker genes were related to wound age. In addition to 0.5 h, there were significant contrasts between the control group and contused group （P<0.05）, about the expression levels of MT1A mRNA and MT2A mRNA in different phases. As the extension of wound age, the relative expression of MT1A mRNA and MT2A mRNA at 1 h, 6 h, 12 h and 18 h after contusion demonstrated upgrade tendency until its expression levels in 18 h peak with 239.41±15.20 and 717.42±50.76, respectively. When time extends to 24 h after injury, the expression of above two marks decreased, respectively. The MT1A mRNA and MT2A mRNA expression levels increased at 30 h and then decreased. Conclusion Determination of MT1A mRNA and MT2A mRNA levels by real-time PCR may be useful for the estimation of wound age.

Key words: forensic pathology, muscles, contusions, metallothionein, wound age estimation

中图分类号: