法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (1): 11-16.DOI: 10.3969/j.issn.1004-5619.2017.01.003

• 论著 • 上一篇    下一篇

心肌缺血-致命性心动过缓血清代谢特征分析

吴嘉燕，王  典，孔  璟，王星星，于晓军   

  1. （汕头大学医学院法医学教研室，广东 汕头 515041）
  • 发布日期:2017-02-25 出版日期:2017-02-28
  • 通讯作者: 王典，男，副研究员，主要从事心源性猝死代谢组学研究；E-mail：g_dwang@stu.edu.cn
  • 作者简介:吴嘉燕（1990—），女，硕士研究生，主要从事法医病理学研究；E-mail：184086295@qq.com
  • 基金资助:

    广东省自然科学基金资助项目（2015A030313437）；广东省科技创新资助项目（2013KJCX0076）

Metabolic Characteristics of Lethal Bradycardia Induced by Myocardial Ischemia

（DEPARTMENT OF FORENSIC PATHOLOGY, MEDICAL COLLEGE, SHANTOU UNIVERSITY, SHANTOU 515041, CHINA）   

  1. WU Jia-yan, WANG Dian, KONG Jing, WANG Xing-xing, YU Xiao-jun
  • Online:2017-02-25 Published:2017-02-28

被引次数

2

摘要： 目的 探讨心肌缺血-致命性心动过缓大鼠血清代谢特征。 方法 制作心肌缺血-心动过缓-死亡（myocardial ischemia-bradycardia-sudden cardiac death，MI-B-SCD）大鼠模型，以假手术组为对照组，应用气相色谱质谱联用仪检测死后血清代谢谱，结合代谢组学策略分析血清代谢特征。 结果 MI-B-SCD大鼠血清代谢轮廓与对照差异显著。与假手术组相比，MI-B-SCD大鼠血清赖氨酸、鸟氨酸、嘌呤、丝氨酸、丙氨酸、尿素、乳酸含量明显升高；琥珀酸、棕榈酸、酮己二酸、甘油醛、己烯二酸、辛二酸含量明显下降，其中多种差异代谢物之间存在相关性。 结论 MI-B-SCD大鼠血清代谢显著改变，赖氨酸与嘌呤有较高的诊断MI-B-SCD价值，可望为心源性猝死的法医鉴定及临床防治提供参考。

关键词: 法医病理学, 心肌缺血, 心动过缓, 血清, 代谢

Abstract: Objective To explore the metabolic characteristics of lethal bradycardia induced by myocardial ischemia in rat’s serum. Methods A rat myocardial ischemia-bradycardia-sudden cardiac death （MI-B-SCD） model was established, which was compared with the sham-operation group. The metabolic profile of postmortem serum was analyzed by gas chromatography-mass spectrometry （GC-MS）, coupled with the analysis of serum metabolic characteristics using metabolomics strategies. Results The serum metabolic profiles were significantly different between the MI-B-SCD rats and the control rats. Compared to the control rats, the MI-B-SCD rats had significantly higher levels of lysine, ornithine, purine, serine, alanine, urea and lactic acid; and significantly lower levels of succinate, hexadecanoic acid, 2-ketoadipic acid, glyceraldehyde, hexendioic acid and octanedioic acid in the serum. There were some correlations among different metabolites. Conclusion There is obvious metabolic alterations in the serum of MI-B-SCD rat. Both lysine and purine have a high value in diagnosing MI-B-SCD. The results are expected to provide references for forensic and clinical applications of prevention and control of sudden cardiac death.

Key words: forensic pathology, myocardial ischemia, bradycardia, serum, metabolic

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