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法医学杂志 2018, Vol. 34 Issue (1) :18-22    DOI: 10.3969/j.issn.1004-5619.2018.01.004
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Nrf2蛋白在骨骼肌损伤修复中的时序性表达及作用
张小红1,2,刘  季3,李  娜1,杜秋香1,王英元1,孙俊红1
1. 山西医科大学法医学院,山西 太原 030001; 2. 邹平县公安局,山东 邹平 256200; 3. 广州市公安局交通警察支队,广东 广州 510000
Time-dependent Expression of Nrf2 Protein and its Effects on Wound Repair in Skeletal Muscle
ZHANG Xiao-hong1,2, LIU Ji3, LI Na1, DU Qiu-xiang1, WANG Ying-yuan1, SUN Jun-hong1
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China; 2. Zouping Public Security Bureau, Zouping 256200, China; 3. Traffic Police Detachment of Guangzhou Municipal Public Security Bureau, Guangzhou 510000, China

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摘要 目的 通过检测骨骼肌损伤后核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)蛋白的表达变化,探讨其在骨骼肌损伤修复过程中的表达变化规律及作用。 方法 取雄性SD大鼠腓肠肌内注射心脏毒素,制作骨骼肌损伤模型,分别取正常对照组和损伤后1 h、4 h、8 h、12 h、16 h、1 d、3 d、5 d、7 d、9 d、13 d、17 d、21 d的损伤处组织,采用HE染色法观察骨骼肌损伤修复过程中的形态学变化,检测活性氧(reactive oxygen species,ROS)相对含量,Western印迹法及免疫荧光染色检测Nrf2蛋白表达水平。 结果 大鼠骨骼肌损伤后,损伤区依次出现以中性粒细胞、单核细胞为主的炎症细胞浸润,损伤后5 d大量新生肌细胞生成,至21 d基本完成肌细胞修复。与正常对照组相比,大鼠骨骼肌损伤后:ROS相对含量仅第12 h降低(P<0.05);Nrf2蛋白16 h表达升高(P<0.05);Nrf2阳性核率在损伤后增多,16 h~1 d达到高峰,随后逐步减少,最后恢复至对照水平(P<0.05)。 结论 Nrf2蛋白参与了骨骼肌损伤修复的调控,Nrf2阳性核率随损伤时间呈时序性变化,可用于骨骼肌损伤时间的推断。
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张小红
刘季
李娜1
关键词法医病理学;肌   骨骼;活性氧;核因子E2相关因子2;氧化性应激;损伤时间推断     
Abstract: Objective To detect the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) to explore its change rule and effects in the repair process of skeletal muscle. Methods An injury model of skeletal muscle was established by injection of cardiotoxin into the gastrocnemius muscle of SD male rats. The muscle samples were taken from control group and injury group at each time point (1 h, 4 h, 8 h, 12 h, 16 h, 1 d, 3 d, 5 d, 7 d, 9 d, 13 d, 17 d, 21 d). The morphological changes of skeletal muscle in repair process were observed by H&E staining, and the content of reactive oxygen species (ROS) was detected. The expression level of Nrf2 protein was investigated by Western blotting and immunohistochemistry staining. Results After the skeletal muscle injury, the injury region was infiltrated with inflammatory cells which constituted mainly by lymphocyte neutrophils and mononuclear cells. There were large amounts of immature muscle cells appeared at the 5th day and the muscle repair was completed at the 21th day. Comparing with control group, the ROS content decreased in injury group at the 12th hour (P<0.05), while Nrf2 expression increased at the 16th hour (P<0.05). Moreover, the positive rate of Nrf2 increased after injury, and peaked at 16 h-1 d, then decreased gradually and returned to the level of control group (P<0.05). Conclusion The Nrf2 protein involves in the regulation of the repair of skeletal muscle injury. The positive rate of Nrf2 shows a time-dependent expression, which can be used to estimate the wound age of skeletal muscle.
Keywordsforensic pathology   muscle, skeletal   reactive oxygen species   nuclear factor erythroid 2-related factor 2   oxidative stress   wound age estimation     
Fund:

国家自然科学基金面上资助项目(81571852);山西省回国留学人员科研资助项目(2015-052)

Corresponding Authors: 王英元,男,博士,教授,主要从事法医病理学研究;E-mail:wyy580218@163.com 孙俊红,男,博士,副教授,主要从事损伤病理学和猝死病理学研究;E-mail:sunjunhong146@163.com   
About author: 张小红(1982—),男,硕士研究生,主要从事损伤病理学研究;E-mail:20021323zhang@sina.com
引用本文:   
张小红, 刘季, 李娜,等.Nrf2蛋白在骨骼肌损伤修复中的时序性表达及作用[J]  法医学杂志, 2018,34(1): 18-22
ZHANG Xiao-Hong, LIU Ji, LI Na-1,et al.Time-dependent Expression of Nrf2 Protein and its Effects on Wound Repair in Skeletal Muscle[J]  , 2018,34(1): 18-22
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