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法医学杂志 2018, Vol. 34 Issue (1) :13-17    DOI: 10.3969/j.issn.1004-5619.2018.01.003
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混合效应模型在玻璃体液推断死亡时间中的应用
杨明真1,李辉军2,张天叶3,丁自娇1,吴士凡1,邱鑫罡1,刘  茜1
1. 华中科技大学同济医学院法医学系,湖北 武汉 430030; 2. 华中科技大学同济医学院附属同济医院检验科,湖北 武汉 430030; 3. 上海市公安局物证鉴定中心 上海市现场物证重点实验室,上海 200083
Application of Mixed-effect Model in PMI Estimation by Vitreous Humor
YANG Ming-zhen1, LI Hui-jun2, ZHANG Tian-ye3, DING Zi-jiao1, WU Shi-fan1, QIU Xin-gang1, LIU Qian1
1. Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China; 2. Clinical Lab, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China; 3. Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China

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摘要 目的 通过检测不同温度下家兔尸体玻璃体液内K+、Mg2+浓度随PMI的变化规律,探索应用混合效应模型推断死亡时间(postmortem interval,PMI)的可行性。 方法 家兔处死后分别置于5 ℃、15 ℃、25 ℃和35 ℃温度下保存,在0~120 h内每12 h双眼交替微量提取玻璃体液80~100 μL。应用生化免疫分析仪检测玻璃体液中K+、Mg2+的浓度。应用混合效应模型进行分析拟合,建立PMI推断方程。使用放置于10 ℃、20 ℃、30 ℃温度下均经过20、40、65 h的样本检测数据对PMI推断方程进行验证。 结果 各温度(y)下家兔玻璃体液中K+、Mg2+浓度[f(x,y)]随PMI(x)延长均呈上升趋势。在5 ℃~35 ℃,PMI及温度拟合的K+、Mg2+浓度方程分别为:f■(x,y)=3.413 0+0.309 2 x+0.337 6 y+0.010 83 xy-0.002 47 x2(P<0.000 1);f■(x,y)=0.745 6+0.006 432 x+0.033 8 y(P<0.000 1)。经验证,PMI为0~40 h时,K+、Mg2+浓度推断PMI的偏离时间均在10 h以内;PMI为40~65 h时,偏离时间在21 h以内。 结论 在5 ℃~35 ℃的环境温度区间内,利用混合效应模型拟合的方法可实现利用温度和玻璃体液物质浓度双参数推断PMI,将为解决玻璃体液化学物质在PMI推断中的实际应用提供新方法。
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杨明真
李辉军
张天叶
关键词法医病理学   玻璃体   死亡时间   体液   混合效应模型           
Abstract: Objective To test the changes of the potassium (K+) and magnesium (Mg2+) concentrations in vitreous humor of rabbits along with postmortem interval (PMI) under different temperatures, and explore the feasibility of PMI estimation using mixed-effect model. Methods After sacrifice, rabbit carcasses were preserved at 5 ℃, 15 ℃, 25 ℃ and 35 ℃, and 80-100 μL of vitreous humor was collected by the double-eye alternating micro-sampling method at every 12 h. The concentrations of K+ and Mg2+ in vitreous humor were measured by a biochemical-immune analyser. The mixed-effect model was used to perform analysis and fitting, and established the equations for PMI estimation. The data detected from the samples that were stoned at 10 ℃, 20 ℃ and 30 ℃ with 20, 40 and 65 h were used to validate the equations of PMI estimation. Results The concentrations of K+ and Mg2+ [f(x,y)] in vitreous humor of rabbits under different temperature increased along with PMI (x). The relative equations of K+ and Mg2+ concentration with PMI and temperature under 5 ℃~35 ℃ were f■(x,y)=3.413 0+0.309 2 x+0.337 6 y+0.010 83 xy-0.002 47 x2 (P<0.000 1), and f■(x,y)=0.745 6+0.006 432 x+0.033 8 y (P<0.000 1), respectively. It was proved that the time of deviation for PMI estimation by K+ and Mg2+ was in 10 h when PMI was between 0 to 40 h, and the time of deviation was in 21 h when PMI was between 40 to 65 h. Conclusion In the ambient temperature range of 5 ℃-35 ℃, the mixed-effect model based on temperature and vitreous humor substance concentrations can provide a new method for the practical application of vitreous humor chemicals for PMI estimation.
Keywordsforensic pathology   vitreous body   postmortem interval   body fluids   mixed-effect model   potassium   magnesium     
Fund:

证据科学教育部重点实验室(中国政法大学)开放基金资助项目(2014KFKT06);中央高校基本科研业务费资助项目(HUST:2015TS003)

Corresponding Authors: 刘茜,女,副教授,博士,主要从事法医病理学和法医毒理学研究;E-mail:caixe_liu0222@tom.com   
About author: 杨明真(1990—),女,硕士研究生,主要从事法医病理学和法医毒理学研究与鉴定;E-mail:yangmingzhen90@126.com
引用本文:   
杨明真, 李辉军, 张天叶, 等.混合效应模型在玻璃体液推断死亡时间中的应用[J]  法医学杂志, 2018,34(1): 13-17
YANG Ming-Zhen, LI Hui-Jun, ZHANG Tian-Ye, DENG ,et al..Application of Mixed-effect Model in PMI Estimation by Vitreous Humor[J]  , 2018,34(1): 13-17
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