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法医学杂志 2018, Vol. 34 Issue (1) :7-12    DOI: 10.3969/j.issn.1004-5619.2018.01.002
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皮肤切创愈合过程中FoxO1表达与损伤时间关系
陈  扬1,2,3,季心怡1,2,3,范琰琰1,2,3,喻林升1,2,3
1. 温州医科大学法医学系,浙江 温州 325035; 2. 温州医科大学司法鉴定中心,浙江 温州 325035; 3. 温州医科大学司法鉴定科学技术研究所,浙江 温州 325035
Relationship between FoxO1 Expression and Wound Age during Skin Incised Wound Healing
CHEN Yang1,2,3, JI Xin-yi1,2,3, FAN Yan-yan1,2,3, YU Lin-sheng1,2,3
1. Department of Forensic Medicine, Wenzhou Medical University, Wenzhou 325035, China; 2. Judicial Forensic Center, Wenzhou Medical University, Wenzhou 325035, China; 3. Institute of Forensic Science, Wenzhou Medical University, Wenzhou 325035, China

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摘要 目的 检测皮肤切创愈合过程中FoxO1的表达及其时间规律性变化。 方法 建立小鼠皮肤切创模型,应用免疫组织化学技术及Western印迹法检测切创后不同时间段皮肤中FoxO1的表达。 结果 HE染色显示皮肤创口正常愈合。免疫组织化学结果显示,对照组FoxO1弱表达于表皮、毛囊、皮脂腺、血管内皮及真皮中少数的成纤维细胞;伤后6~12 h创口周边区表皮及皮肤附件FoxO1表达增强,可见浸润的中性粒细胞和少量单核细胞表达FoxO1;伤后1~3 d FoxO1以单核细胞阳性表达为主;伤后5~10 d FoxO1以新生血管内皮及成纤维细胞表达为主;伤后14 d仍有少量成纤维细胞表达FoxO1。Western印迹法检测显示,创伤各组创口皮肤的FoxO1表达量均高于对照组,其中伤后12 h和伤后7 d为FoxO1的两个表达峰值。 结论 FoxO1在皮肤切创愈合过程中呈现一定的时序性变化,有望成为推断创口形成时间的生物学指标。
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陈扬
季心怡
范琰琰
关键词法医病理学   创伤和损伤   皮肤   创口愈合   叉头框蛋白O1   损伤时间推断     
Abstract: Objective To investigate FoxO1 expression and its time-dependent changes during the skin incised wound healing. Methods After the establishment of the skin incised wound model in mice, the FoxO1 expression of skin in different time periods was detected by immunohistochemistry and Western blotting. Results Immunohistochemistry staining showed that FoxO1 was weakly expressed in a few fibroblasts of epidermis, hair follicles, sebaceous glands, vessel endothelium and dermis in the control group. The FoxO1 expression was enhanced in the epidermis and skin appendages around the wound during 6-12 h after injury, which could be detected in the infiltrating neutrophils and a small number of monocytes. FoxO1 was mainly expressed in monocytes during 1-3 d after injury, and in neovascular endothelial cells and fibroblasts during 5-10 d. On the 14th day after injury, the FoxO1 expression still could be detected in a few fibroblasts. The Western blotting results showed that the FoxO1 expression quantity of the tissue samples in injury group was higher than in control group. The FoxO1 expression peaked at 12 h and 7 d after injury. Conclusion FoxO1 is time-dependently expressed in skin wound healing, which can be a useful marker for wound age determination.
Keywordsforensic pathology   wounds and injuries   skin   wound healing   forkhead box O1   wound age determination     
Fund:

国家自然科学基金资助项目(81301640);浙江省自然科学基金资助项目(Q13H150005)

Corresponding Authors: 喻林升,男,博士,教授,主要从事皮肤损伤时间推断的研究;E-mail:yls@wmu.edu.cn   
About author: 陈扬(1991—),男,硕士研究生,主要从事皮肤损伤时间推断的研究;E-mail:1021763645@qq.com
引用本文:   
陈扬, 季心怡, 范琰琰, 等.皮肤切创愈合过程中FoxO1表达与损伤时间关系[J]  法医学杂志, 2018,34(1): 7-12
CHEN Yang, JI Xin-Yi, FAN Yan-Yan, et al. .Relationship between FoxO1 Expression and Wound Age during Skin Incised Wound Healing[J]  , 2018,34(1): 7-12
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