Relationship between FoxO1 Expression and Wound Age during Skin Incised Wound Healing
CHEN Yang1,2,3, JI Xin-yi1,2,3, FAN Yan-yan1,2,3, YU Lin-sheng1,2,3
1. Department of Forensic Medicine, Wenzhou Medical University, Wenzhou 325035, China; 2. Judicial Forensic Center, Wenzhou Medical University, Wenzhou 325035, China; 3. Institute of Forensic Science, Wenzhou Medical University, Wenzhou 325035, China
Objective To investigate FoxO1 expression and its time-dependent changes during the skin incised wound healing. Methods After the establishment of the skin incised wound model in mice, the FoxO1 expression of skin in different time periods was detected by immunohistochemistry and Western blotting. Results Immunohistochemistry staining showed that FoxO1 was weakly expressed in a few fibroblasts of epidermis, hair follicles, sebaceous glands, vessel endothelium and dermis in the control group. The FoxO1 expression was enhanced in the epidermis and skin appendages around the wound during 6-12 h after injury, which could be detected in the infiltrating neutrophils and a small number of monocytes. FoxO1 was mainly expressed in monocytes during 1-3 d after injury, and in neovascular endothelial cells and fibroblasts during 5-10 d. On the 14th day after injury, the FoxO1 expression still could be detected in a few fibroblasts. The Western blotting results showed that the FoxO1 expression quantity of the tissue samples in injury group was higher than in control group. The FoxO1 expression peaked at 12 h and 7 d after injury. Conclusion FoxO1 is time-dependently expressed in skin wound healing, which can be a useful marker for wound age determination.