法医学杂志

法医学杂志 ›› 2020, Vol. 36 ›› Issue (3): 337-340.DOI: 10.12116/j.issn.1004-5619.2020.03.008

• 技术与应用 • 上一篇    下一篇

中空纤维超滤技术在死后溶血血液总IgE检测中的应用

王天琦1， 贾宇晴1， 刘添翼2， 刘逸凡2， 曹志鹏1， 朱宝利1   

  1. 1. 中国医科大学法医学院法医病理学教研室，辽宁 沈阳 110122； 2. 中国医科大学临床三系，辽宁 沈阳 110122
  • 发布日期:2020-06-25 出版日期:2020-06-28
  • 通讯作者: 朱宝利，男，教授，博士研究生导师，主要从事法医病理学、法医病理生理学教学、研究及鉴定；E-mail：zhu1127@hotmail.com
  • 作者简介:王天琦（1994—），男，硕士研究生，主要从事法医病理学、法医病理生理学研究；E-mail：1252527890@qq.com
  • 基金资助:
    国家自然科学基金资助项目（81273343）；辽宁省科技厅自然科学基金面上项目（20180530004）

Application of Hollow Fiber Ultrafiltration Technology in the Detection of Total IgE of Hemolytic Blood after Death

WANG Tian-qi1, JIA Yu-qing1, LIU Tian-yi2, LIU Yi-fan2, CAO Zhi-peng1, ZHU Bao-li1   

  1. 1. Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110122, China; 2. The Third Clinical Department, China Medical University, Shenyang 110122, China
  • Online:2020-06-25 Published:2020-06-28

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摘要： 目的 探究中空纤维超滤技术对溶血样品的处理效果及超滤液中IgE浓度与溶血前血清浓度的差异。 方法 收集死后72 h内非冷冻尸体的尸检血样33例，每例取4 mL血液，其中1 mL离心保留血清，余3 mL血液冻融3~5次致其完全溶血。将2 mL溶血样品经中空纤维超滤处理得到超滤液。应用文齐氏液-氰化高铁血红蛋白测定法检测血清、全溶血样品及其超滤液中血红蛋白浓度，电化学发光法检测血清、超滤液中总IgE。 结果 超滤液中血红蛋白浓度明显低于全溶血样品（P<0.05）。超滤液和血清中总IgE检测值具有良好相关性（r=0.984），超滤液中IgE校正后数值与血清差异无统计学意义，且两者检测阳性率差异无统计学意义（P>0.05）。 结论 超滤技术对全溶血样品具有良好处理效果且超滤液检测校正值贴近溶血前血清水平，可应用于冷冻尸体溶血样品总IgE检测。

关键词: 法医病理学, 免疫球蛋白E, 溶血, 中空纤维超滤, 血液

Abstract: Objective To investigate the treatment effect of hollow fiber ultrafiltration technology on hemolytic samples and the differences between IgE concentration and serum concentration before hemolysis in ultrafiltrate. Methods The 33 postmortem blood samples of non-frozen corpses within 72 hours after death were collected, 4 mL blood was taken from each case, among which 1 mL was centrifuged to get serum, and the remaining 3 mL blood was frozen-thawed 3-5 times to cause complete hemolysis. The 2 mL hemolytic samples were processed by hollow fiber ultrafiltration to obtain ultrafiltrate. The hemoglobin concentration in serum, complete hemolytic sample and ultrafiltrate was determined by Van-Zij solution-cyanated methemoglobin assay method, and the total IgE in serum and ultrafiltrate was determined by electrochemical luminescence method. Results The hemoglobin concentration in ultrafiltrate was significantly lower than that in complete hemolytic samples （P<0.05）. There was a good correlation between the total IgE detection values of ultrafiltrate and serum （r=0.984）. The difference between the serum and the value of IgE in ultrafiltrate after correction had no statistical significance, and the differences between the two in positive rates had no statistical significance （P>0.05）. Conclusion Ultrafiltration technology has a good treatment effect on complete hemolytic samples, and the correction value of ultrafiltrate detection is close to the serum level before hemolysis, and therefore, it can be applied to the detection of total IgE of frozen corpse hemolytic samples.

Key words: forensic pathology, immunoglobulin E, hemolysis, hollow fiber ultrafiltration, blood