Enzymehistochemical and immunohistological changes of skeletal muscle motor end-plates and muscle fibers and their relation to the time of death

Abstract

Abstract: In order to investigate the relationship between the retrograde changes of the skeletal muscle and the time of death in various postmortem intervals (PMI), a systemic study of the enzymehistochemical activity of ACHE, SDH, LDH, Ca~(2+)-ATPase and the immunohistochemical reaction of SYN in motor end-plates and muscle fibers was conducted in rats under different temperatures and at various PMI. The results were analyzed and compared by an image processing system. It was found that these changes were related to the PMI, especially AChE changes. The AChE could be used as a sign-enzyme of skeletal muscle to date death.

Key words: skeletal muscle, motor end- plate, AchE, enzymhistochemistry, PMI

WANG HUIJUN;ZHANG CHUNMING;XIONG XIKAI;ET AL (DEPARTMENT OF ULTRASTRUCTURAL PATHOLOGY;RESEARCH CENTER OF EXPERIMENTAL MEDICINE;TONGJI MEDICAL UNIVERSITY;WUHAN 000;P.R. CHINA). Enzymehistochemical and immunohistological changes of skeletal muscle motor end-plates and muscle fibers and their relation to the time of death[J]. , 1999, 0(3): 135-137+.

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Enzymehistochemical and immunohistological changes of skeletal muscle motor end-plates and muscle fibers and their relation to the time of death

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