Abstract: In this paper, a method of qualitative analysis and quantitative analysis of common barbiturates in a little biological sample by capillary gas chromatography is described A fine separate condition of gas chromatography was obtuined on OV—1 capillary column and OV—210 capillary column. A rapid and simple method of quantitative analtsis by capillary gas chromatography was established with allobarbitone as internal standard. The retention value of eighteen sedative and tranqilizer on the same condition of gas chromatography was observed.