Abstract: The purpose of this study was to evaluate the practical uses of the Hematoxylin Basic Fuchsin Picnic (HBFP) staining method. Myocardial sections from 26 medical-legal autopsy cases stained with HBFP were observed under the light microscope. The results showed that positive staining with HBFP was consistent with the morphologic changes and the sites of myocardial ischemia as described by previous authors. The results also demonstrated that the method was useful in diagnosing early myocardial ischemia. The problems of staining procedure and false positive results were discussed.

Key words: HBFP staining method, early myocardial ischemia, false positive