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28 April 1990, Volume 6 Issue 2 Previous Issue    Next Issue
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Forensic Pathological Study of Brain Death in 120 Cases and Application of Posterior Arch-Ectomy (P.A.E) of Atlas LI DEXIANG DEPARTMENT OF FORENSIC PATHOLOGY FACULTY OF FORENSIC MEDICINE CHINA MEDICAL UNIVERSITY 1990, 0(2): 1-7+15.  Abstract ( 2059 )   1. Forensic autopsies in 120 cases died from primary intracranial damage were made.2. These cases were divided into the part of primary brain death becauof the antemortem clinical signs of brain (come apnea and the other functions Less of brain) prior to the signs of heart.3. Using the method 《P.A.E》 created by me, it was found that there were 112 cases (93.3%) had herniations of foramen magnam including cerebellar herniation in 71 cases (59.2%) and cerebellar tonsillar medullary oblongata rerniation in 41 cases (34.2%). In which 99 cases (82.5%) had liquefications of the cerebellar tonsillar herniations; 27 cases (22.5%) were found that the fragments of cerebellar tonsillar tissue were fallen into spinal space and 3 cases (2.5%) implanted the spinal cords;4. The one of the distinetive findings in this study was the growth of implanted cerebellar tonsillar tissue in the spinal cords.5. On the Occurence and development of brain death in this series, except Primary intercrinial damages the secondary neuropathological changes especiall the anoxic and ischemic necrosis of cerebral cortex cerebellar cortex and brain stem were important too. Related Articles | Metrics

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The study of individual specificity and somatic stability of DNA fingerprints LAN LIN;ZHANG XIAO-WEI;DUAN BING-ZHANG;HOU ZHENG-YI BEIJING FORENSIC SCIENCE INSTITUTE 1990, 0(2): 8-11.  Abstract ( 1980 )   A blind trial involving 60 blood samples has been successfully carried out. By using minisatellite probe 33.15 we have obtained satisfactory DNA fingerprints. After statistical treatment the probability of individual specificity is calculated as 5.6×10~(-12). Futhermore, we have studied blood, semen and bloodstain of the same individual by probe 33.6, resulting identica DNA fingerprints. It shows somatic and germ-line stability and completely specific to individual. The viewpoint of international standardization of DNA fingerprints has been first proposed in this paper. Related Articles | Metrics

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In The Organism of Dead Rabbit DNA Quantity Has Changed By Fluorimetry XU JUN JIE;QUE TINGZHI INSTITUTE OF FORENSIC SCIENES;MINISTRY OF JUSTICE;PRC 1990, 0(2): 11-15.  Abstract ( 1969 )   This paper reported the method for quantitative determination of DNA by the fluorescent dye of DAPI. To make use of fluorimetry detected directly quantity of DNA in the kidenys, livers, spleens, cardiac muscles and skeletal muscles of rabbit to died by ether. And assied the change of DNA quantity following to seven hours for organism.1. The quantity of DNA was not changed following the postmortem in the kidenys, cardiac and skeletal muscles.2. In the spleens, the quantity of DNA was increased following the postmortem.3. In the livers, the quantity of DNA was deceased following the post. mortem. Related Articles | Metrics

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Evaluation of Hematoxylin Basic Fuchsin Picric (HBFP) Staining Method in Diagnosis of Early Myocardial Ischemia LAI YAO;LIAO ZHIGANG;YEI ZHONGYU DEPARTMENT OF PATHOLOGY;SCHOOL OF FORENSIC MEDICINE;WEST CHINA UNIVERSITY OF MEDICAL SCIENCES 1990, 0(2): 16-18+3.  Abstract ( 1971 )   The purpose of this study was to evaluate the practical uses of the Hematoxylin Basic Fuchsin Picnic (HBFP) staining method. Myocardial sections from 26 medical-legal autopsy cases stained with HBFP were observed under the light microscope. The results showed that positive staining with HBFP was consistent with the morphologic changes and the sites of myocardial ischemia as described by previous authors. The results also demonstrated that the method was useful in diagnosing early myocardial ischemia. The problems of staining procedure and false positive results were discussed. Related Articles | Metrics

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An Ultra-thin PAGIEF Technique used for Phenotyping PGM1 Subtypes WANG XIULING;JIA JINGTAO DEPARTMENT OF FORENSIC SEROLOGY;FACULTY OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 1990, 0(2): 19-20.  Abstract ( 1900 )   In this paper, we recommonded an ultra-thin PAGIEF technique which can be used for phenotyping the PGM1 subtypes. This technique may be able to save a great quantity of stock gel solution, Ampholine and staining solution, and to reduce the operating time significantly. The most samples we used were the freeze red cells reserved over one year. This indicates that the freezing method is important for reserving the reference red cells. Related Articles | Metrics

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Differentiation of Group ABSe Body Fluids (or Stains)from Mixture of Group ASe and BSe Body Fluids(or Stains)by ELISA ZHOU BIN;GUO JINGYUAN DEPARTMENT OF FORENSIC SEROLOGY SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES 1990, 0(2): 21-26+4.  Abstract ( 2012 )   It has been known that blood group substances in body fluids from group AB individuals possess both group A and B activities on the same macromolecules. We applied the results of such studies to differ of group ABSe body fluids (or stains) from mixtures of group ASe and BSe body fluids (or stains) by a sensitive "Sandwich method" enzyme-linked immunosorbent assay (ELISA).The polystyrene ELISA plates were sensitized with monoclonal anti-A or anti-B. A or B substance fixed to plates was quantitatively analysed by the combined use of human anti-A or anti-B and horseradish peroxidase labeled anti-human IgM. The 10000 fold diluted body fluids could be detected by this method. In type AB body fluids, A and B substances were detected with monoclonal anti-A sensitized plate or anti-B sensitized plate. In the mixture of type A and type B body fluids: however, only type A or type B substances were detected with corresponding plate. Related Articles | Metrics

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Identification of pyrethroid insecticides with Gc method 1990, 0(2): 27-30.  Abstract ( 1916 )   Identification of Pyrethroid Insecticides With GC Method (Hu Zengren; Yan Jixiang; Lin Nengming; Pharm acy Department of Zhejiang Medical University, Hanzhou, China)The identification method of six Common pyrethroid insecticides with GC has been developed experimentally. Using relative retention time (RRT) to one compound of this Series in GC as the identification guo may be ideal. Compared with retention index method, this one may identify all pyrethroid insecticides and in simple and rapid. Related Articles | Metrics