›› 1991, Vol. 0 ›› Issue (4): 169-172+.

• 论文 • Previous Articles     Next Articles

THE ANALYSIS OF DNA RFLPs USING M13 BACTERIOPHAGE AS A PROB

ZHANG XIAOWEI, ET AL, BEIJING FORENSIC SCIENCE INSTITUTA BAIJING. (100007)   

  • Online:1991-08-25 Published:1991-08-28

Abstract: The hypervarieble minisatellite regions in human, animal and plant genome DNA can be detected by M13 bacteriophage as a probe.Its effective sequence was traced to two clusters of 15-base pair tandem repeats within the gene 3 of the bacteriophage. In this paper we utilized a unipue method of PCR to label M13 phage DNA and yielded a high specific activity. Human DNA digested with Hinfl was hybridized with labelled M13 and 33.15 probes. Highly polymorphic DNA fingerpriuts were obtained witb both of them. A practical casework was tested. (Original article on page 169)