Abstract: Polymorphism of EsD was investigated by isoelectrical fo- cusing in 14 kinds of organ tissuse,that is,in brain,heart,liv- er,spleen,lung,kidney,pancreas,small intestine,large intes- tine,muscle,skin,ovary,uterus,and testis.The samples were obtained from 7 male and 5female cadavers who were medicolegally autopsied within 48h after death.The results showed that except skin,all the 13 kinds of organs included 5 EsD 1-1,6 EsD 2-1,and 1 EsD 2-2.The results for the determination limits of EsD types in 13 organ tissues stored at 37℃,room temperature and 4℃ showed that all the tissues examined were correctly typed for EsD at 37℃ for periods of up to 3 days,at room temperature for periods of up to 3 weeks and at 4℃ for periods of up to 5 weeks.