Abstract: By means of electron microscopic enzy- me—histochemical method,we studied ouabain-sensitive,potassium-dependent ATPase in rat epidermal incised wound.The enzyme reac- tion activities on the inner side of membranes of capillary and outer root sheath of epidermal decreased in antemortem injury.ATPase activities were not changed in wound of postmortem injury. This study has important value in diagnosing injury and early age of wound.