Abstract: This article describes a sensitive method that detects morphine,6-monoacetylmorphine,morphine-3-glucuronide and codeine in urine for qualifying the abuse of heroin.The analytes were extracted by solid phase C18. The limits of detection (LOD) for morphine and codeine were 50ng/ml and 50ng/ml,respectively.The RSD of morphine and codeine were 11.3%(n=5),and 14.2%(n=5) respectively.For urine,it does not need to be hydrolyzed before extracted,and for all analytes,also need not to be derivated.The difference ratio of morphine and codeine in the chromatography can be used to discriminate between the abuse of heroin and the administration of compound liquorice mixture.

Key words: heroin abuse