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28 April 1999, Volume 15 Issue 2 Previous Issue    Next Issue
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The changes of GFAP,PCNA after brain contusion:an immunohistochemical study in forensic pathology WU XU;WANG DEWEN;ZHANG GUOHUA;ET AL (DEPARTMENT OF FORENSIC PATHOLOGY; CHINA MEDICAL UNIVERSITY;SHENYANG 000;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1673 )   After the model of experimental brain contusion was set up,the changes of GFAP,PCNA were studied by immunohistochemistry SP method combined with image quantity analysis.After contusion,the gray degrees and areas of GFAP-positive cells increased significantly at the 3rd hour,reached the highest level in the 4th day and kept until the 7th day.PCNA-positive cells appeared in the 12th hour, simultaneously, their gray degrees tended to increase and positive areas tended to decrease. It showed regular changes of GFAP,PCNA with various survival times, and these changes were useful in the diagnosis of brain contusion especially in the period from the second to 7th day after injury.The numbers of astrocytes increased and reached the highest level in the 3rd day after contusion while the reactive proliferation of astrocytes is only a minor phenomenon. Related Articles | Metrics

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MBP content in serum after closed diffuse cranial injury in rats YU XIAOJUN;CHEN GUODI;GOU QING;ET AL(DEPARTMENT OF FORENSIC MEDICINE;JILIN MEDICAL COLLEGE;JILIN 00;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1876 )   The MBP content change in serum was observed by ELISA in rats with experimental closed diffuse cranial injury.The MBP content was 6.1633± 1.5301ng/ml(X±S) in the normal group,11.3818±2.6574ng/ml in the first group died immediately after injury, 10.8319±2.3135ng/ml in the second group of 15 min after injury . This increased level of MBP was lasting in the following 3 days and returned to normal at the 4th and 5th day after injury. The difference of the MBP levels between the groups after injury and that in the normal group is significant (P<0.01). Related Articles | Metrics

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Forensic medical identification of sudden death due to tuberculosis of adrenal glands GONG YUCHANG;QIN ZHIQIANG (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1696 )   Two cases died of tuberculosis of adrenal glands were reported. The pathological findings are presented and their medico-legal significance was also discussed. In particular, emphasis is placed on the importance of histopathologic examination of the adrenal glands in the tuberculous subjects dying suddenly and in whom no obvious cause of death was found at necropsy. Related Articles | Metrics

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Forensic pathology studies of sudden death caused by pulmonary embolism WEI DENGMIN;YAN HUAIQI;WANG ZHAOSHENG;ET AL (DEPARTMENT OF PATHOLOGY;ZUNYI MEDICAL COLLEGE; GUIZHOU 00;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1856 )   The analytic result of 50 sudden death cases caused by pulmonary embolism showed that their main causes were trauma (42%), heart diseases (32%), surgical operation (20%) and so on. In majority of these cases (80%), thrombus embolus came from deep veins of lower limbs, in some cases (20%) came from cardiac cavity. These cases occurred most frequently in the period of October to December and rarely in the period of April to June. Finally, some problems in forensic examination in this sort of sudden death were discussed in this article. Related Articles | Metrics

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Immunohistochemical observation of actin in viral myocarditis CHENG JIANDING;CHEN YUCHUAN;HU BINGJIE (DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 00;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1954 )   10 specimens of heart in autopsy were immunohistochemically studied by using anti-actin(At) antibody. In 5 cases with definite or suspected viral myocarditis according to Dallas criteria, actin in myocardium showed focal or diffuse weak staining, and occasionally showed focal absence of staining. In 2 cases with myocardial infarction, actin showed large pieces absent of staining, while actin in 3 cases with non-cardiac death showed strongly positive, well-distributed staining. The results indicated the immunohistochemical observation of actin in myocardium is useful for improving the sensitivity in diagnosing viral myocarditis and differentiating the slight injuries of myocytes caused by ischemia or inflammation. Related Articles | Metrics

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Quantification of female teenagers' competence of sexual cognition WU JIASHEN;GUAN WEI; LAO NANYI (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1856 )   Compiling a questionnaire of sexual cognition for surveying 224 female teenagers (11 to 14 years old),the results showed that they were deficient in the knowledge of sexual physiology, morality and law.And also,the deficiency had a high co-relationship with the age of surveyed female teenagers.It suggested that this questionnaire be valuable in the quantification of sexual cognition. Related Articles | Metrics

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Allele and genotype frequency distribution of D19S400 locus in several populations CHEN GUODI;HOU YIPING;LI YINGBI;ET AL (INSTITUTE OF FORENSIC MEDICINE;WEST CHINA UNIVERSITY OF MEDICAL SCIENCE;CHENGDU 00;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1904 )   Using polymerase chain reaction and PAG electrophoresis,D19S400 Locus was investigated in the populations of Chinese,Germany,Slovakian,and American Black Population.620 samples collected from the four populations were tested and a total of 11 alleles (*7 to *17)and 47 genotypes were found.The heterozygosity was observed between 0.78 and 0.88,and the discriminating power was between 0.9385 and 0.9664.The distributions of genotypes in the four populations were in accordance with Hardy-Weinberg equilibrium.Significant differences were observed in the distribution of allele frequencies among the three major racial populations (Mongoloid,Caucasian,Negroid).According to the results obtained in this study,D19S400 locus is a robust STR for forensic and genetic purposes. Related Articles | Metrics

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Research on DNA typing in the forensic identification of formalin-fixed paraffin embedded tissues LIN YUAN;ZHENG SONGGUO;LU XIAOYU;ET AL (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1916 )   Successful extraction of DNA from formalin-fixed Paraffin embedded tissues is important to DNA typing for forensic purpose.In the research,26 samples were analyzed by method of PM and HLA-DQA1 loci typing.24 of these samples were successful.It showed that this technique and method could be applied to individual identification and paternity test by use of these sorts of samples. Related Articles | Metrics

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Polymorphic study of CSF1PO locus in Han population in Wuhan CHEN HUI;YU CHUNYING;YANG RONGZHI;ET AL (DEPARTMENT OF FORENSIC BIOLOGY;TONGJI MEDICAL UNIVERSITY; WUHAN 000;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1921 )   Using the primers designed by Yoshida, the amplified fragment length polymorphism of the STR locus CSF1PO was studied. 8 alleles were determined from 312 unrelated individuals of Han population living in Wuhan. Sensitivity and suitability of the PCR-STR typing using Yoshida's primers were compared with the one using Hammond's. Results proved that Yoshida's method were more suitable in forensic identification than that of Hammond, especially in the typing of severely degraded DNA samples. Related Articles | Metrics

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Polymorphism and hapletype study of Y-chromosomal multi-STRs loci in Han population in Guangzhou TANG SHUANGBO;GUO JINGYUAN;LIU CHAO ET AL (DEPARTMENT OF FORENSIC SCIENCES;SUN YAT-SEN UNIVERSITY;GUANGZHOU 00;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1716 )   Using polymerase chain reaction and silver stain, polymorphism and hapletype study of Y-chromosomal multi-STRs loci: DYS19, DYS389I/II, DYS390 were studied. 111 samples of male were collected from Guangzhou area. 5 alleles were determined in DYS19 locus, 4 alleles in DYS389I locus, 5 alleles in DYS389II locus and 5 alleles in DYS390 locus. Compared with other racial populations, differences of distribution of allele frequencies existed significantly. 72 hapletype were present in this study. Related Articles | Metrics

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Application of solid-phase microextraction technique to the detection of amphetamines in urine by GC LIU WEI;SHEN MIN (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE; SHANGHAI 000;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 2188 )   A simple and rapid detection of nine amphetamines coexisting in urine was described.In the test,the method of solid-phase micro-extraction (SPME) by GC technique was used.Urine (1.0ml),NaCl (0.3g) and 4-phenylbutylamine (internal standard) were added into a vial (1.5ml),then the sample was adjusted to pH 12 with 10% NaOH and sealed with a teflon-coated septum.After immersion of the SPME fiber (100 PDME) in the sample for 15 min,the SPME needle was inserted into the injection port of the GC and extruded for 3min. The result showed that each peak from nine amphetamines compounds and internal standard was clearly separated.The calibration curves were linear from 0.2 to 15μg/ml for most of five amphetamines with r between 0.9928-0.9995.The CV were less 10%. It is concluded that the method is simple,quick,accurate and useful for the practical detection of urine concentration of amphetamines. Related Articles | Metrics

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Analysis of fluoroacetamide in biological samples with GC/NPD, GC/FID FENG SHIZHEN;YU ZHONGSHAN (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF PUBLIC SECURITY;BEIJING 000;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1905 )   A GC/NPD and GC/FID method has been developed for the analysis of fluoroacetamide (1081),using AC-20 and FFAP capillary column.The recovery is 85.1%(RSD 5.4%), 85.1%(RSD 7.1%) and 81.6%(RSD 10.1%) in liver,whole blood and stomach tissue,respectively. Related Articles | Metrics

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Determination of metabolites of heroin in urine and discrimination of heroin abuse WU HEJIAN;SHEN MING;XIAN PING;ET AL(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 1999, 0(2): 0-0.  Abstract ( 1875 )   This article describes a sensitive method that detects morphine,6-monoacetylmorphine,morphine-3-glucuronide and codeine in urine for qualifying the abuse of heroin.The analytes were extracted by solid phase C18. The limits of detection (LOD) for morphine and codeine were 50ng/ml and 50ng/ml,respectively.The RSD of morphine and codeine were 11.3%(n=5),and 14.2%(n=5) respectively.For urine,it does not need to be hydrolyzed before extracted,and for all analytes,also need not to be derivated.The difference ratio of morphine and codeine in the chromatography can be used to discriminate between the abuse of heroin and the administration of compound liquorice mixture. Related Articles | Metrics