Abstract: Using polymerase chain reaction and PAG electrophoresis,D19S400 Locus was investigated in the populations of Chinese,Germany,Slovakian,and American Black Population.620 samples collected from the four populations were tested and a total of 11 alleles (*7 to *17)and 47 genotypes were found.The heterozygosity was observed between 0.78 and 0.88,and the discriminating power was between 0.9385 and 0.9664.The distributions of genotypes in the four populations were in accordance with Hardy-Weinberg equilibrium.Significant differences were observed in the distribution of allele frequencies among the three major racial populations (Mongoloid,Caucasian,Negroid).According to the results obtained in this study,D19S400 locus is a robust STR for forensic and genetic purposes.

Key words: short tandem repeat, D19S400