Abstract: Using the primers designed by Yoshida, the amplified fragment length polymorphism of the STR locus CSF1PO was studied. 8 alleles were determined from 312 unrelated individuals of Han population living in Wuhan. Sensitivity and suitability of the PCR-STR typing using Yoshida's primers were compared with the one using Hammond's. Results proved that Yoshida's method were more suitable in forensic identification than that of Hammond, especially in the typing of severely degraded DNA samples.

Key words: STR, PCR, CSF1PO locus, amplified fragment length polymorphism