Multiplex PCR fluorescent detection of DNA Loci and its application in forensic science

›› 2000, Vol. 0 ›› Issue (1): 14-15+6.

Multiplex PCR fluorescent detection of DNA Loci and its application in forensic science

QUE TINGZHI,ET AL.(INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE, SHANGHAI 200063,P.R.CHINA)

Online:2000-02-25 Published:2000-02-28

Abstract

Abstract: A method is reported using PCR multiplex amplification and fluorescent-labeled primers to genotype D3S1358、D16S539、TH01、TPOX、CSF1PO、D7S820 and Amelogenin loci. It is simple,accurate,sensitive and very useful for personal identification and paternity testing.

Key words: D3S1358, D16S539, TH01, TPOX, CSF1PO, D7S820, amelogenin, fluorescence detection

QUE TINGZHI;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA). Multiplex PCR fluorescent detection of DNA Loci and its application in forensic science[J]. , 2000, 0(1): 14-15+6.

[1]	YANG Yi-zun, PING Yuan. Analysis of CSF1PO and D18S51 Loci Based on Ion Torrent PGMTM Platform [J]. 法医学杂志, 2018, 34(5): 520-525.
[2]	BI Jie, CHANG Jing-jing, YU Chun-ying. Detection and Analysis of 12 Suspected Amelogenin Allelic Loss Cases [J]. 法医学杂志, 2018, 34(4): 396-400.
[3]	HUANG JIANG-PING, YANG FAN, LIU YA-NAN, ET AL.. Research Progress on Gene Alterations of Amelogenin Locus in Gender Identification [J]. , 2016, 32(5): 371-377.
[4]	WANG SAN-CUN, DING MEI-MAN, WEI XIAO-LIN, ET AL.. Recognition of Y Fragment Deletion by Genotyping Graphs after Amplified by PowerPlex?誖21 Detection Kit [J]. , 2016, 32(3): 193-195.
[5]	ZHANG HU-QIN;YANG ZHOU-QI;LIU FANG-E;ZHANG JIN;ZHAO WEN-MING(.THE KEY LABORATORY OF BIOMEDICAL INFORMATION ENGINEERING OF MINISTRY OF EDUCATION;SCHOOL OF LIFE SCIENCE AND TECHNOLOGY;XI′AN JIAOTONG UNIVERSITY;XI′AN 00;CHINA;.THE CENTER. Homologous Amelogenin Gene Test of Archaeological Samples [J]. , 2006, 0(3): 213-216.
[6]	WU WEN－QING;JIN YING (FU DAN MEDICAL UNIVERSITY;SHANGHAI 000). Sex identification in forensic medicine [J]. , 2001, 0(3): 189-192.
[7]	LIN YUAN;ET AL (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 00;P R CHINA). Personal identification using multiplex PCR and fluorescent detection of STR and Amelogenin locus [J]. , 2000, 0(2): 75-76+1.
[8]	QUE TINGZHI;LI LI;LIN YUAN;ET AT (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P. R. CHINA). Population study of the CSF1PO, TPOX and TH01 STR polymorphisms in east China [J]. , 1999, 0(3): 144-146+.
[9]	JIANG BIN;GUO JINGYUAN;LIANG SHANGQIU (DEPARTMENT OF FORENSIC. SCIENCE;THE SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCE;GUANGZHOU 00;P. R. CHINA). PCR-based DNA analysis of vWA31A, TH01, F13A01, FESFPS, TPOX and CSF1PO [J]. , 1999, 0(3): 141-143+.
[10]	CHEN HUI;YU CHUNYING;YANG RONGZHI;ET AL (DEPARTMENT OF FORENSIC BIOLOGY;TONGJI MEDICAL UNIVERSITY; WUHAN 000;P.R.CHINA). Polymorphic study of CSF1PO locus in Han population in Wuhan [J]. , 1999, 0(2): 0-0.
[11]	JL DU CYJIANG BING;GUO JIN YUAN(DEPARTMENT OF FORENSICMEDICINE.SUN-YAL SEN UNIVERSITY OF MEDICINESCIENCE; ZHONGSHAN ROAD;GUANGZHOU 00). Determination of sex using PCR of the X-Y homologous gene amelogeuin [J]. , 1997, 0(2): 68-70+1.

Multiplex PCR fluorescent detection of DNA Loci and its application in forensic science

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics