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28 February 2000, Volume 16 Issue 1 Previous Issue    Next Issue
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Polymorphism of 13 STR loci for establishment of chinese criminal DNA database DU ZHICHUN;ET AL. (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 2000, 0(1): 1-5+61.  Abstract ( 2137 )   Allele frequency distributions for thirteen STR loci: D3S1358,vWA,FGA,D8S1179,D21S11,D18S51,D5S818,D13S317,D16S539,THO1,TPOX,CSF1PO,and D7S820,have been determined by multiplex amplification and subsequent automatic fluorescent detection. The result of statistical analysis shows that the 13 loci satisfy Hardy-Weinberg expectation. The data obtained from this study will be used as reference data for forensic DNA analysis in China. Related Articles | Metrics

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Immunohistochemical studies on vascular endothelial growth factor (VEGF) for the postmortem diagnosis of sudden coronary death CHEN JIANGUO;ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 00;P.R.CHINA 2000, 0(1): 6-7+63.  Abstract ( 1917 )   The expression of VEGF in myocardium of the heart of sudden coronary death (SCD; n=16) and of the control group (n=15) was studied quantitatively by immunohistochemistry SABC method, image analysis and statistical process. The results showed that local infarcted myocardium of the heart from all SCD had strong positive expression of VEGF, especially in peri-infarction area. The rate of positive expression was 100%, while only two of the 15 in control group had mild positive expression sparsely in local myocardium. Image analysis and statistical process showed that the positive index in SCD group was 13.68±2.73, and in the control group 2.05±0.84. There was a significant difference between the two groups (P< 0.01). The results suggested that the immunohistochemical staining of VEGF with image analysis and statistical process be used as an objective marker for the postmortem diagnosis of SCD. Related Articles | Metrics

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An experimental study on the relationship between early postmortem intervals and DNA content of spleen cells in rats by computerized image analysis LIU LIANG;ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL UNIVERSITY;WUHAN 000;P.R.CHINA 2000, 0(1): 8-9+11.  Abstract ( 1853 )   rats were used in this experiment. After the rats were executed,spleen cells were collected and smeared in every hour within 24 hours, then fixed with formalin solution and stained with Feulgen′s method. Mean and integral optical density and abnormal index of cell DNA were measured with a auto-TV-image system, and the data were statistically analyzed. Results showed that the DNA degeneration rate of rat spleen cells had a linear relationship with early postmortem intervals. Related Articles | Metrics

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Expression of bcl-2 and Bax protein in the failing human heart —an inhibitor or accelerator of apoptosis? CHEN YIJIU;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 2000, 0(1): 10-11+6.  Abstract ( 1905 )   In general, myocyte death in myocardial infarctions (MI) is attributed to necrosis, but recently the involvement of apoptosis has been suggested. Loss of myocytes is an important mechanism in the development of cardiac failure of either ischemic or nonischemic origin. The expression of two proto-oncogenes influence apoptosis. Myocytes with positive bcl-2 immunoreactivity were seen in the heart of acute MI, localized only in the salvaged areas surrounding the infarcted tissues. Myocytes with overexpressed positive Bax immunoreactivity were observed in the salvaged areas of old MI. Related Articles | Metrics

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Validation studies on the analysis of the HLA-DQA1 locus using PCR and its application in forensic science. LIN YUAN;ET AL.(INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 2000, 0(1): 12-13+6.  Abstract ( 2029 )   Validation Studies were conducted to evaluate the forensic applicability of HLA-DQA1 locus. The locus was typed by a reverse dot blot approach and allele specific oligonucleotide probes were immobilized on a nylon membrane strip. Allele and genotype frequency for HLA-DQA1 locus was determined in shanghai Han population. The frequency data can be used in individual identification and paternity test. Related Articles | Metrics

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Multiplex PCR fluorescent detection of DNA Loci and its application in forensic science QUE TINGZHI;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 2000, 0(1): 14-15+6.  Abstract ( 2037 )   A method is reported using PCR multiplex amplification and fluorescent-labeled primers to genotype D3S1358、D16S539、TH01、TPOX、CSF1PO、D7S820 and Amelogenin loci. It is simple,accurate,sensitive and very useful for personal identification and paternity testing. Related Articles | Metrics

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DNA typing of old skeletons and teeth with fluorescence tagged short tandem repeats YE JIAN;ET AL. (INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF PUBLIC SECURITY;BEIJING 000;P.R.CHINA) 2000, 0(1): 16-17+2.  Abstract ( 2034 )   In order to identify the individual status of old human skeletons and teeth, studies were conducted on DNA extraction. The extracted DNA was then amplified and automatic typed with 373A sequence′s gene scan system (made by PE Company) and fluorescence tagged Hum vWA, Hum LPL kit. This is a rapid, convenient and effective method in DNA typing of old skeletons and teeth in forensic investigation. Related Articles | Metrics

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The application of minisatellite MS31A MVR-PCR digital coding technique in forensic science HOU GUANGWEI;ET AL.(FORENSIC SCIENCE INSTITUTE OF LIAONING PROVINCE;SHENYANG 00;P.R.CHINA) 2000, 0(1): 18-20+6.  Abstract ( 2110 )   Using isotope incorporate amplification technique of special 31A, 31A-A, 31A-G primers and α-32PdCTP, the minisatellite MS31A (located at D7S21 loci) was studied . A rapid, simple,and accurate MVR-PCR technique was successfully established. The technique can be applied in individual identification minutes amples, such as blood stains, semen stains contaminated by vaginal fluid, hair and bones. The sensitivity analysis revealed that this technique could detect 1ng genoma DNA. It is also discribed about the application of the method in 40 criminal cases of rape and murder. Related Articles | Metrics

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Determination of fenfluramine in corpse using GC/NPD and GC/MS SUN YAJUAN;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;P.R.CHINA) 2000, 0(1): 21-23+6.  Abstract ( 2016 )   A method was described for the determination of fenfluramine by GC/MS in blood, urine, liver, lung, kidney and heart tissues. After liquid-liquid extraction of fenfluramine in the specimens obtained from autopsy, the samples were qualitatively identified by GC/MS, and quantitatively determined by GC/NPD. 4-phenyl butylamine was used as internal standard. The concentrations of fenfluramine in blood, urine and liver were 7.8μg/ml, 64.2 μg/ml and 31.3 μg/g respectively. The findings of autopsy and the results of toxicological analysis were also discussed. Related Articles | Metrics

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Evaluation of competence for serve a sentence HUANG FUYING;ET AL.(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;PRC SHANGHAI 000;P.R.CHINA) 2000, 0(1): 38-40+6.  Abstract ( 2075 )   In order to explore the factors influencing the evaluation of competence for serve a sentence, 102 cases of forensic psychiatric expertise were reviewed. A questionnaire of scale of adaptability to punishment circumstance" was designed to investigate these cases. The results showed that the patients of psychotic mental disorders and the patients with history of mental disorders have a relatively high rate of non-competence of serving sentences, and that they can not adapt theirself to punishment circumstance . Related Articles | Metrics