The application of minisatellite MS31A MVR-PCR digital coding technique in forensic science

Abstract

Abstract: Using isotope incorporate amplification technique of special 31A, 31A-A, 31A-G primers and α-32PdCTP, the minisatellite MS31A (located at D7S21 loci) was studied . A rapid, simple,and accurate MVR-PCR technique was successfully established. The technique can be applied in individual identification minutes amples, such as blood stains, semen stains contaminated by vaginal fluid, hair and bones. The sensitivity analysis revealed that this technique could detect 1ng genoma DNA. It is also discribed about the application of the method in 40 criminal cases of rape and murder.

Key words: minisatellite MS31A MVR-PCR, individual identification

HOU GUANGWEI;ET AL.(FORENSIC SCIENCE INSTITUTE OF LIAONING PROVINCE;SHENYANG 00;P.R.CHINA). The application of minisatellite MS31A MVR-PCR digital coding technique in forensic science[J]. , 2000, 0(1): 18-20+6.

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The application of minisatellite MS31A MVR-PCR digital coding technique in forensic science

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