Abstract: In order to find out changes of GFAP and PCNA after human brain trauma, samples of 38 cases were studied by the immunnohistochemistry method combined with quantitative image analysis The gray degree and area of PCNA positive cells increased to the maximum at the 4th day and then decreased gradually GFAP positive cells began to increase significantly 12 hours after contusion, reached the maximum at the 4th day and stayed in high level until the 7th day These results suggest that: (1) GFAP, PCNA show regular changes with survival time after brain contusion;(2) GFAP and PCNA can be used as time markers of brain contusion from 2-20 days

Key words: brain trauma, immunnohistochemistry, image analysis (Original article on page 137)