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28 June 2000, Volume 16 Issue 3 Previous Issue    Next Issue
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The effect of injuries on Fn synthesized by FBs and its implication in wound timing SHEN YIWEN;ET AL( DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL UNIVERSITY;SHANGHAI 000;PRC) 2000, 0(3): 129-131+.  Abstract ( 1998 )   cFn synthesized by FBs around the wound was detected by immunochemistry in vitro Using the method of ELISA, The concentration of cFn in the culture media was observed The concentration of cFn had no change within 30 minutes after injury but started to increase after 1 hour of the injury and kept this trend within next five hours after injury By static analysis, it can be obtained that the concentration of cFn in culture media is time-dependent after injury Related Articles | Metrics

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A study of HSP70 and NF in brain contusion timing WANG HUIJUN;ET AL (DEPARTMENT OF PATHOLOGY;SHANGHAI MEDICAL UNIVERSITY;SHANGHAI 000;PRC) 2000, 0(3): 132-134+.  Abstract ( 2079 )   The changes of HSP70 and NF after brain contusion were studied by the immunohistochemistry method HSP70 and NF were seen at 30 min after injury around the wound area, but faded away after 12-24h The result suggests that these two proteins could be used to estimate brain contusion time, to diagnose brain wound and to distinguish antemortem and postmortem brain contusion Related Articles | Metrics

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Analysis of the type, reason for death and cause of non-malpractice tangle ZHANG YIGU (DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL UNIVERSITY;WUHAN 000;PRC) 2000, 0(3): 135-136+.  Abstract ( 2034 )   autopsy cases related to the death of non-malpractice medical tangle from 1972 to 1998 were studied retrospectively 111 (53 88%) were died of natural diseases, 82 (38 81%) inevitable complications and 13 (6 31%) unpredictable medical accidents For the cause of medical tangles, the diagnosis of 92 cases (44 66%) was questioned The other disputes focused on the infusion/blood transfusion (31 cases, 15 05%), the injection (20 cases, 9 71%), drug misuse and delay in making diagnosis or giving treatment (18 cases, 8 74%respectively) 13 cases (13 11%) involved complains on medical staff's rudeness and hospital management Related Articles | Metrics

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The changes of GFAP and PCNA after human brain contusion: an immunohistochemical study in forensic pathology TAO LUYANG;ET AL ( INSTITUTE OF FORENSIC SCIENCES OF SHENYANG;SHENYANG 000;PRC) 2000, 0(3): 137-138+.  Abstract ( 1795 )   In order to find out changes of GFAP and PCNA after human brain trauma, samples of 38 cases were studied by the immunnohistochemistry method combined with quantitative image analysis The gray degree and area of PCNA positive cells increased to the maximum at the 4th day and then decreased gradually GFAP positive cells began to increase significantly 12 hours after contusion, reached the maximum at the 4th day and stayed in high level until the 7th day These results suggest that: (1) GFAP, PCNA show regular changes with survival time after brain contusion;(2) GFAP and PCNA can be used as time markers of brain contusion from 2-20 days Related Articles | Metrics

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An experimental study on the effect of positional asphyxia on cardiac and pulmonary functions CHEN YUCHUAN;ET AL (SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 00;PRC) 2000, 0(3): 139-140+.  Abstract ( 1924 )   The purpose of the study is to explore the effect of positional asphyxia on cardiac and pulmonary functions and its role in causing death respiration,pulmonary artery pressure and cardiac output were measured by inserting the swan-ganz into the pulmonary artery from the femoral vein Results showed that: (1) the respiration was stable before hanging, getting faster during hanging, and becoming irregular at the terminal stage of hanging; (2) the pulmonary artery pressure increased significantly during and at the terminal stage of hanging as compared with that before hanging;(3) the cardiac output was stable before and during hanging, but decreased significantly at the terminal stage of hanging It is concluded that hanging may cause respiratory imbalance and hypoxia, resulting in cardiac and pulmonary dysfunction The death caused by hanging belongs to respiratory death, the characteristic of which is primary respiratory arrest followed by secondary cardiac arrest Related Articles | Metrics

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Expression of ET of early ischemic myocardium in rats MA MENGYUN;ET AL (SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 00;PRC) 2000, 0(3): 141-142+.  Abstract ( 2076 )   To study the expression of ET of early ischemic myocardium, we designed a model of early myocardial ischemia (EMI) in rats The study was conducted with SABC method and HE staining Cardiac myocytes of ischemia showed a little ET-positive at 60-min Cardiac myocytes, endothelium and smooth muscle of vessel showed ET-positive staining in the area of EMI after 120-min Staining was negative for Ischemia myocardium at 30-min and for the control group No change was seen with HE staining in all groups The data indicates that ET-SABC method may reveal the injury of myocardial ischemia 60-min and longer and may provide a new marker to diagnose EMI in forensic pathology Related Articles | Metrics

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Automated fluorescent analysis of STR profiling and sex determination JIANG BIN;ET AL (LEGAL MEDICAL EXPERTISE CENTER OF HENGYANG MEDICAL COLLEGE;HENGYANG 00;PRC) 2000, 0(3): 143-145+.  Abstract ( 1867 )   Denaturing PAGE coupled with the ABI377 fluorescent automated DNA sequencer was used to test the performance and reproducibility of the automated DNA profiling systems at vWA31A, TH01, F13A01, FES, TPOX, CSF1PO and Amelogenin gene The allele designation windows at the 7 genetic markers were established and implemented into the genotype reading software Alleles differing in just 1bp in length could easily be discriminated Furthermore, the interpretation guidelines were outlined for the 7 genetic systems by investigating the relative peak areas of heterozygote peaks and relative stutter peak areas in various monoplex systems Our results indicate that if the ratio between two peaks is equal to or higher than 0 404, a herozygote could be determined, otherwise the homozygote be made Related Articles | Metrics

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The MN typing of whole blood and bloodstains by the method of one-step sandwich ELISA BAO LEI;ET AL (LIAONING BASIC MEDICAL INSTITUTE;SHENYANG 000;PRC) 2000, 0(3): 146-147+.  Abstract ( 1974 )   The samples of whole blood and bloodstain were MN-typed by the method of one-step sandwich ELISA, monoclonal antibodies include anti-M, N and glycernprotein The lowest sample amount in this study is: for whole blood is 0 065μl, for bloodstain about 10 to 50ng 455 cases of fresh blood and 200 cases of fresh bloodstain are correctly typed For 58 cases of old bloodstains , the success rate is 96 6% Related Articles | Metrics

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Detection of Antide pressantand Antipsychotic Drugsin Human Hair and Evaluationof the Results SHENMIN;ETAL (INSTITUTEOFFORENSICSCIENCES;MIN ISTRYOFJUSTICE;SHANGHAI000;PRC) 2000, 0(3): 148-152+.  Abstract ( 1664 )   Thepresenceoftherapeuticdrugsinthehairofpsy chiatricpatientswasinvestigated,andthemethodfor simultaneousdeterminationofantidepressantandan tipsychoticdrugsbyGC/NPDandGC/MSwasalsoes tablished Eightdifferentpsychotroicdrugsweredetect edfromthehairof32subjects,andchlorpromazineand clozapineconcentrationsinthehairwerefoundtobede pendentonthedosageandthecorrelationcoefficients were0 8047(P<0 001,n=16)and0 7097(P<0 001, n=16)respectively Segmentalanalysisdemonstrated thattherewasacorrelationbetweenthesubject'sdrug exposurehistoryandthedistributionofdrugalongthe hairshaft Related Articles | Metrics

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Determination of Lidocaine in Human spinal cord by High - performance Liquid Chromatography JINGMING;ETAL (FACULTYOFFORENSICMEDICINE;TONGJI MEDICALUNIVERSITY;WUHAN000;PRC) 2000, 0(3): 153-154+.  Abstract ( 1558 )   ARPHPLCmethodfordetermininglidocaineinhu man′sspinalcord Wasestablished Thelinearrangeof themethodwas0 20 -20 0μg/ml(r=0 9999) Thedetec tionlimitwas0 02ng/ml(S/N≥3)andtheclean -upre coveryoflidocaineinhumanspinalcordwas82 4% - 92 7% TheCV %werenomorethan6 0%(N=4) No interferebyothercompoundswasobservedinstudyre sultsusingthismethod RPHPLCmethodshowsanex cellentrecovery,highsensitivityandgoodprecision Related Articles | Metrics