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28 August 2000, Volume 16 Issue 4 Previous Issue    Next Issue
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Evaluation of hearing thresholds of 40Hz auditory event related potential and auditory brainstem response FAN LIHUA;ET AL. (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000) 2000, 0(4): 193-195+.  Abstract ( 2121 )   Recordings of pure tone response, 40Hz auditory event－ related potential (40Hz AERP) and auditory brainstem response (ABR) were obtained for 74 ears of 42 cases (32 normal ears, 42 injured ears). 40Hz AERP(0.5～ 2kHz) of 20 ears was tested under natural sleep and awake conditions. The results showed that the threshold of 40Hz AERP was higher than that of tone pip, with the difference of 12.7± 6.4dBnHL at 0.5kHz, 14.7± 6.3dBnHL at 1kHz, and 15.6± 5.6dBnHL at 2kHz respectively. The threshold of 40Hz AERP increased in natural sleep compared with that in awake state. The threshold of ABR was higher than the behavioral. The data suggests that jointly using several tests get more accurate and objective results in evaluating hearing loss. Related Articles | Metrics

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Motor evoked potentials of upper limbs ZHU GUANGYOU;SHEN YAN (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000) 2000, 0(4): 196-197+.  Abstract ( 1998 )   This study aims to explore a method to record and analyze motor evoked potentials (MEP) of upper limbs. Compound muscle action potentials (CMAP) are recorded from abductor digiti minimi (ADM) and abductor pollicis brevis (APB) by magnetic stimulation of human brain and peripheral nervous. Results show that the latency of left and right Cz－ APB is 25.23ms and 25.19ms respectively.The difference of the bilateral latencies is 2.24ms. The rate of amplitude, the rate of area for bilateral sides is 0.45 and 0.37. The left and right central conduction time (CCT) of Cz－ APB is 11.79ms and 11.75ms. The bilateral difference is 2.00ms. The latency of left and right Cz－ ADM is 25.23ms and 25.19ms respectively. The difference of bilateral latencies is 2.24ms. The rate of amplitude, the rate of area for bilateral sides is 0.45 and 0.37. The left and right CCT of Cz－ ADM is 11.79ms and 11.75ms.The bilateral difference is 1.86ms. Related Articles | Metrics

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Development and application of the software measuring the size of injured body surface YU XIAOJUN;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE OF NORTH CHINA UNIVERSITY;JILIN 00) 2000, 0(4): 201-204+.  Abstract ( 2111 )   A software to measure the area, length and angle of injured body surface is introduced. It is suitable for evaluating injury degrees with 2 or 3 dimension measurement. The software is developed by Visual Basic 6.0 on Windows 98 terrace, supporting digital video camera, digital camera and scanner input.It is easy to use and has been applied in more than 200 cases. Related Articles | Metrics

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Immunohistochemical study of basic fibroblast growth factor of early myocardial infarction HU BINGJIE;ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 00) 2000, 0(4): 205-207+.  Abstract ( 2042 )   The expression of basic fibroblast growth factor (bFGF) was first studied in 61 cases of early myocardial infarction. The results revealed that all 24 cases of proven infarcts was positive in nuclei of myocardium and vascular endothelium, and that some was positive in cytoplasm, especially in myocardium around the border zone of infarction. Of 21 suspected infarcts cases, 17 showed strong positive and 4 mild positive. In the control group of 16 samples, only 3 was mild positive sparsely in nuclei of myocardium and vascular endothelium. PI of control group was significantly lower than that of proven and suspected infarcts (P< 0.01). There was no significant difference between PI of proven infarcts and suspected infarcts (P >0.05). These findings suggest that SABC－ bFGF method could be used for morphologic diagnosis of early myocardial infarction of sudden cardiac death. Related Articles | Metrics

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Expression of TGF－β_1 and proliferation of collagen in myocardium in viral myocarditis CHENG JIANDING;ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 000) 2000, 0(4): 208-209+.  Abstract ( 1859 )   To investigate the tissue repair of acute viral myocarditis,and to explore the diagnostic method of slight viral myocarditis in forensic pathology. Slight viral myocarditis model was induced in Balb/c murine by CVB3. Collagen proliferation in myocardium of mice with myocarditis was observed by special staining.The hearts of mice and human(9 cases) with myocarditis were studied LSAB－ immunohistochemically with anti－ TGF－β 1 antibody.In the study, the proliferation of collagen was seen in myocardium in acute viral myocarditis. Generous expression of TGF－β 1 was found in the myocardium of mice and human with myocarditis. The quantity of collagen proliferation and expression of TGF－β 1 was positive correlation. It is concluded that the tissue repair exists in acute viral myocarditis and that positive staining of myocardium for TGF－β 1 is a sensitive index of myocardial damage and tissue repair. Related Articles | Metrics

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Experimental study on basic fibroblast growth factors in viral myocarditis ZHA GUOQING;ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUNGZHOU 000) 2000, 0(4): 210-213+.  Abstract ( 1830 )   In order to know earlier pathological changes in myocarditis， 8 rats were inoculated with CVB3, and a model of viral myocarditis was built. The changes of basic fibroblast growth factors (bFGF) in acute viral myocarditis were observed by immunohistochemistry. It was found that the bFGF stain started to show positive at the third day after viral infection and that the positive degree increased afterwards. With normal HE staining, obvious lesion usually appeared under microscopy at least five days after infection. This suggests that the changes of bFGF in myocardium can be used for examination of early and light lesions of viral myocarditis. Related Articles | Metrics

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Study on histology and the expression of Bax and Bcl－ 2 after cerebral contusion in rats CHEN LONG;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY SHANGHAI 000;) 2000, 0(4): 211-213+.  Abstract ( 2089 )   To set up the experimental model of focal cerebral contusions in rats,and to study the histology and the expression of Bax and Bcl－ 2 after cerebral catusions.It was found that neurons and astrocytes around the wound area were changed morphologically and that polymorphonuclear leucocytes were present at 12－ 24h after injury. A lot of foamy cells and astrocytes having a big dark nucleus appeared around the wound area on the 4th day after injury. On the 8－ 10th day, liquefaction necrosis was seen in areas of contusions. On the 12－ 14th day, the contusion focus healed up and became cystiform or glial node. Meantime, lots of astrocytes, capillary vessel and hemosiderin were observed. The expression of Bax and Bcl－ 2 increased after injury. There existed a relationship between increased Bax/Bcl－ 2 and different intervals after brain injury. Related Articles | Metrics

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Usefulness of Cofiler PCR Amplification Kit in Parentage Testing LI LI;ET AL. (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI;000) 2000, 0(4): 214-215+.  Abstract ( 1973 )   Parentage testing was performed by using multiple PCR and automated genescan and genotype analysis. Parentage was excluded in 36 cases,including 22 of 149 paternity trios and 14 of 37 paternity duos. In the 22 trios and 14 duos in which paternity was excluded, a mean of 6.1 and 5 incompatible STR markers were noted. The application value of six loci which included in the Cofiler amplification kit was showed here. The discrimination power ranged from 0.8023 for TPOX to 0.9203 for D16S539. The cumulative power of exclusion was provided as 0.9825. Three mutation were found (one each at D3S1358,TPOX,CSF1PO). For most nonexcluded paternity duos, only when the Cofiler amplification kit was used in conjunction with Profiler Plus kit, the RCP value was acceptable. The data showed that the Cofiler kit could play an important role in cases of paternity duos. Related Articles | Metrics

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Evaluation of 9 STR Loci in paternity identification LIU YAN;ET AL. (INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;SHANGHAI 000) 2000, 0(4): 216-218+.  Abstract ( 2069 )   9 STR loci obtained by four－ dye fluorescent labeling technique in paternity identification provides much information at one test and the cumulative chance of exclusion gets up to 0.9999. Our result of 268 paternity test cases shows that there are at least two incompatible loci in all Mother－ Child－ Alleged Father (M－ C－ AF) exclusive cases. To those unexclusive cases, The RCP all reaches international standard. It is suggested that more STR loci be used for accurate test in Child－ Alleged Father(C－ AF) case. Related Articles | Metrics