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28 February 2001, Volume 17 Issue 1 Previous Issue    Next Issue
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Study on the expression of fibronectin after cerebral contusion in rats for timing of injuries CHEN LONG;ET AL.( DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000) 2001, 0(1): 1-3+61.  Abstract ( 2021 )   An experimental model of reproducible focal cerebral contusions in rats was made by a free－ drop impacting right hemisphere. The expression of fibronectin and its mRNA after cerebral contusion were detected respectively by immunohistochemical staining and in situ hybridization. Results indicated that the expression of fibronectin and its mRNA increased after injury, and there existed a relationship between increased fibronectin and its mRNA and different intervals after brain injury. It is inferred that the expression of fibronectin and its mRNA can be used for timing of brain injuries and distinguishing antemortem and postmortem brain contusions. Related Articles | Metrics

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Histopathological observation of experimental slight viral myocarditis CHENG JIANDING;ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU;GUANGDONG 000) 2001, 0(1): 4-6+61.  Abstract ( 2052 )   To study the diagnostic method of slight viral myocarditis in the field of forensic pathology, slight viral myocarditis model was induced in Balb/c murine by coxsackie virus B3. Organs of hearts, livers, spleens, lungs and kidneys were examined through routine pathological methods. Pathological changes at different levels of these organs were observed. The results indicated that viral myocarditis was a kind of disease with multiple organ alterations and that the pathological observation and comprehensive analysis of multiple organs was one of the useful methods for diagnosing slight viral myocarditis. Related Articles | Metrics

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Study on the specificity of complement C_5 for the postmortem diagnosis of myocardial infarction HU BINGJIE;ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU;GUANGDONG 00) 2001, 0(1): 7-9+61.  Abstract ( 2093 )   In order to explore the specificity of complement C5 in the postmortem diagnosis of myocardial infarction, changes of C5 staining in normal, infarcted and other non－ infarcted myocardia with direct or indirect myocardial injuries (myocarditis, mechanical asphyxia, electrocution, hemorrhagic shock, cardiac contusion and organophosphate poisoning) were studied with immunohistochemistry and image analysis. The results showed that positive C5 staining could be observed in groups of myocardial infarction and myocarditis, but not in groups of mechanical asphyxia, electrocution, hemorrhagic shock, cardiac contusion, and organophosphate poisoning. It is indicated that positive reaction of C5 could only be affected by myocarditis, which means that it was more specific for the diagnosis of myocardial infarction. Related Articles | Metrics

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Histochemical observation of changes of neurons and axons in injured brainstem DENG PING;ET AL.(RESEARCH INSTITUTE OF NEUROSCIENCE;GUANGZHOU MEDICAL COLLEGE;GUANGZHOU;GUANGDONG 0) 2001, 0(1): 10-11+6.  Abstract ( 1904 )   Brainstem of rats were stabbed with a needle and pathological changes of neurons and axons in brainstem were observed at different time after injury with Nissl′ s body staining, silver staining and modified trichrome staining. It was found that, by silver staining, the axons showed irregular swelling and disconnection at 1～ 3h, marked swelling of the severe end at 6h, retraction ball at 15h and remarkable retraction ball at 24h. By modified trichrome staining, the space between myelin sheaths and axons was widened at 3～ 6h, and tortuous myelin sheaths adhered incompletely on axons, or even peeled off at 15h to 24h. Perinuclear lysis of Nissl′ s bodies at 24h after injury could be seen by Nissl body staining. The results indicated that, the pathological changes in injured brainstem could be observed with histochemical staining, which might be used for timing brainstem injuries. Related Articles | Metrics

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Forensic application of reverse dot blot with biotin incorperation JIANG WEIBIN;ET AL.(FORENSIC SCIENCE INSITITUTE OF LIAONING PROVINCE;SHENYANG 00) 2001, 0(1): 12-14+6.  Abstract ( 2091 )   A rapid and accurate reverse dot blot technique was successfully established. It could distinguish the 6 alleles of 0101, 0102, 0103, 0201, 0301 and 0401 of HLA－ DQA locus. Alleles and genotype frequencies for HLA－ DQA locus were determined in 200 unrelated individuals of northern Han population. The discrimination power (DP) was 0.928, heterozygocity (H) 0.75. By direct biotin incorperation, 1ng genome DNA could be detected successfully. This method can be used for paternity test and individual identification in forensic science practice. Related Articles | Metrics

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A study of genetic polymorphism of the STR locus D2S441 in Chinese Han population in Chengdu HUANG DAIXIN;ET AL.(DEPARTMENT OF FORENSIC BIOLOGY;WEST CHINA UNIVERSITY OF MEDICAL SCIENCES;CHENGDU;SICHUAN 00) 2001, 0(1): 15-16+1.  Abstract ( 2041 )   Using PCR and PAG, followed by silver staining, the tetrameric STR D2S441 locus was studied in 260 unrelated Chinese individuals living in Chengdu. 9 alleles and 26 genotypes were observed. The range of fragment size was 131bp to 155bp. The genotype distribution of D2S441 locus in Han population was in accordance with Hardy－ Weinberg equilibrium. Family survey confirmed Mendelian inheritance of alleles. The discriminating power (Dp), observed heterozygosity (H), polymorphism information content (PIC) and power of exclusion (PE) were 0.9084, 0.7885, 0.7390 and 0.5778 respectively. The results demonstrated that this locus was highly polymorphic and could be used for forensic identification and paternity testing. Related Articles | Metrics

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Cantonese population data of the Tetranucleotide repeat locus D7S809 and its application in paternity test LU DEJIAN;ET AL.(FACULTY OF FORENSIC MEDICINE;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU;GUANGDONG 00) 2001, 0(1): 17-18+6.  Abstract ( 1982 )   The tetranucleotide repeat locus D7S809 was investigated in Cantonese population by polymerase chain reaction (PCR) amplification, subsequent polyacryramide gel electrophoresis and silver staining. 14 alleles and 50 genotypes were detected in 190 sample individuals. All alleles differed in size by 4bp repeat. No interalleles were found. The heterozygosity, power of discrimination and chance of non－ paternal exclusion were 0.8613, 0.9645 and 0.7184 respectively. No significant deviations from Hardy－ Weinberg equilibrium were found. The D7S809 was a highly polymorphic, stable and accurate type locus and had been successfully applied to 100 paternity test cases. Related Articles | Metrics

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Prospective Study of Lethal Blood Concentration of Organophosphorous in Humans BU JUN;ET AL.( INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000) 2001, 0(1): 21-24+6.  Abstract ( 2104 )   Total cases of organophosphorous（ dichlorvos、 methamidophos、 dimethoate） poisoning outpatients from six hospitals during four years were collected consecutively for lethal blood concentration study. Blood samples were detected with gas chromatography. The probabilities of death, coma were analyzed with Bliss method and their linear regressive equations of probit were obtained respectively, their 50％ lethal concentrations(LC50) and 50％ coma concentrations(CC50) were calculated by the formulas above. As the death rate was influenced by therapy, its natural death probability has been discussed and estimated their natural LC50 were between the LC50 and CC50 themselves. Combined LC50 and CC50, their natural LC50 were calculated. Related Articles | Metrics

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The Forensic determination of Post－ Traumatic Epilepsy in 21 Patients LIU LING (YUEYANG INTERMEDIATE PEOPLE′ S COURT;HUNAN 000) 2001, 0(1): 25-27+6.  Abstract ( 1926 )   To evaluate the forensic determination of post cerebral traumatic epilepsy. Methods In 21 patients, traumatic history and previous history were analysied combined with the demonstrations of electroencephalogram(EEG), X－ ray, CT and MRI. Results Post－ traumatic epilepsy,manily in late stage, usually occurred following serious cerebral trauma. The type of traumatic epilepsy was determined by the traumatic location and extent. Abnormal epileptic wave in scalp EEG and 24h dynamic EEG and medical image examinations were helpful for qualitative analysis. Conclusion The forensic determination of post traumatic epilepsy must be on the basis of traumatic and previous history combined with EEG, CT and MRI analysis. Related Articles | Metrics

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Analysis on characters of 220 cases of tympani membrane perforated XU ZHAOYANG (WANGCHENG PEOPLE'S COURT;HUNAN 000) 2001, 0(1): 28-29+3.  Abstract ( 1832 )   cases of tympani membrane perforated were summarized retrospectively. The tympani membrane perforated of injury was different from otitis media's because they had marked or extreme marked difference in sex, age, ear distinction, shape, position, size, degree of hearing damaged, time of healing, cause of perforating and adhesive substance. This difference was relative to mechanism of tympani membrane perforated. The distinction gist of two sorts of tympani membrane perforated was expounded through comparison and analysis. It would be of great value to clinical forensic medical examination. Related Articles | Metrics