Forensic application of reverse dot blot with biotin incorperation

Abstract

Abstract: A rapid and accurate reverse dot blot technique was successfully established. It could distinguish the 6 alleles of 0101, 0102, 0103, 0201, 0301 and 0401 of HLA－ DQA locus. Alleles and genotype frequencies for HLA－ DQA locus were determined in 200 unrelated individuals of northern Han population. The discrimination power (DP) was 0.928, heterozygocity (H) 0.75. By direct biotin incorperation, 1ng genome DNA could be detected successfully. This method can be used for paternity test and individual identification in forensic science practice.

Key words: biotin incorperation, PCR, HLA－ DQA, reverse dot blot

JIANG WEIBIN;ET AL.(FORENSIC SCIENCE INSITITUTE OF LIAONING PROVINCE;SHENYANG 00). Forensic application of reverse dot blot with biotin incorperation[J]. , 2001, 0(1): 12-14+6.

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[6]	DENG XIAO-DONG, ZHANG WEI, ZHANG BO, ET AL.. Expression of XPG Gene in Forensic Age Estimation [J]. , 2016, 32(6): 415-419.
[7]	吕YE-HUI , MA KAI-JUN, LI ZHI-HONG,ET AL.. Correlation between RNA Expression Level and Early PMI in Human Brain Tissue [J]. , 2016, 32(4): 245-249.
[8]	LIU YA-JU, ZHANG JUN-TAO, JIN HAI-YING, ET AL.. Establishment and Verification of 6-color Fluorescent-labeled Rapid PCR Amplification System [J]. , 2016, 32(2): 109-113.
[9]	LU ZHI-YONG, XUE LU-YAN, ZHANG QING-XIA, ET AL.. Validation of GlobalFiler?誖PCR Amplification Kit and the STR Polymorphism [J]. , 2015, 31(4): 273-176.
[10]	HU WEI, CHEN RONG-HUA, ZHANG CHEN, ET AL.. Species Identification and Absolute Quantification of Biological Samples by Droplet Digital PCR [J]. , 2014, 30(5): 342-345.
[11]	ZOU KAI-NAN, CAO YU, XIA ZI-FANG, ET AL. Forensic Application of Expressmarker 22 STR Loci Direct PCR Amplification Kit [J]. , 2012, 28(6): 448-450.
[12]	DENG JIAN-QIANG, LIU BAO-QIN, CAI JI-FENG, ET AL. Degenerate Oligonucleotide Primed-PCR Technology Establishment and Its Sensitivity Test Analysis [J]. , 2012, 28(1): 41-43.
[13]	XU YAN, JIANG WEI, PING YUAN, ET AL. Determination of Bloodstain Formation Time by RNA Analysis [J]. , 2010, 26(5): 340-342.
[14]	HE PENG-XIANG;TANG YUN-LIN;CAI WEI-LIN;HUANG YONG-HUA;FANG CHAO;JING HUA-LAN (. DEPARTMENT OF FORENSIC MEDICINE;ZHONGSHAN MEDICAL COLLEGE;SUN YAT-SEN UNIVERSITY;GUANGZHOU 000;CHINA;. THE PLA AIR FORCE HOSPITAL OF GUANGZHOU;GUANGZHOU 00;C. The Quantitative Analysis of Ub and UbE1 in Focus of Traumatic Epilepsy [J]. , 2010, 0(2): 84-87.
[15]	LIU QIU-LING;L DE-JIAN;ZHAO HU (DEPARTMENT OF FORENSIC MEDICINE;ZHONGSHAN MEDICAL COLLEGE;SUN YAT-SEN UNIVERSITY;GUANGZHOU 000;CHINA). Development and Application of X -chromosomal STR Multiplex Amplification System [J]. , 2009, 0(6): 425-427.

Forensic application of reverse dot blot with biotin incorperation

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