Comparison of Application of MD-VF-Auto SEM Method and Plankton Gene Multiplex PCR System in the Diagnosis of Drowning

doi:10.12116/j.issn.1004-5619.2021.410605

Abstract

Abstract: Objective

To compare the application effect of microwave digestion - vacuum filtration - automated scanning electron microscopy （MD-VF-Auto SEM） method and plankton gene multiplex PCR system in the diagnosis of drowning.

Methods

Lung, liver and kidney tissue of 10 non-drowning cases and 50 drowning cases were prepared for further MD-VF-Auto SEM method analysis and plankton gene multiplex PCR system analysis. The positive detection rate of the two methods in each tissue was calculated.

Results

The positive rate of the MD-VF-Auto SEM method detecting diatoms in drowning cases was 100%, and few diatoms were detected in the liver and kidney tissues of 6 non-drowning cases. By using the plankton gene multiplex PCR system, the diatom positive rate of drowning cases was 84%, and all the non-drowning cases were negative. There were significant differences in the positive rate of the liver, kidney tissues between MD-VF-Auto SEM method and plankton gene multiplex PCR system （P<0.05）, as well as the total positive rate of cases. However, no significant differences were found in the positive rates of lung tissues （P>0.05）.

Conclusion

MD-VF-Auto SEM method is more sensitive than plankton gene multiplex PCR system in diatom test. But the plankton gene multiplex PCR system can also detect plankton other than diatoms. Combination of the two methods can provide a more reliable basis for the diagnosis of drowning.

Key words: forensic pathology, drowning, diatom test, plankton, multiplex PCR system, microwave digestion-vacuum filtration-automated scanning electron microscopy method

CLC Number:

DF795.1

Jian-miao ZHANG, Tian-chun LIN, Zhen-yu LIAO, Yu-kun DU, Zhong-hao YU, Jing-jian LIU, Sai-qun WU, Xiao-dong KANG, Qu-yi XU, He SHI, Jian ZHAO, Chao LIU, Dang-en GU. Comparison of Application of MD-VF-Auto SEM Method and Plankton Gene Multiplex PCR System in the Diagnosis of Drowning[J]. Journal of Forensic Medicine, 2022, 38(1): 114-118.

Figures/Tables 3

References 21

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序号	引物	PCR产物长度/bp	荧光标记
1	rPOD-A	101	FAM
2	ND-A	113	FAM
3	rPOD-B	117	FAM
4	CBR	132	FAM
5	ND-B	172	FAM
6	HLYA-A	105	HEX
7	AER-A	111	HEX
8	UPA	166	HEX
9	AER-B	175	HEX
10	HLYA-B	190	HEX
11	AER-C	213	HEX
12	HLYA-C	368	HEX

序号	引物	PCR产物长度/bp	荧光标记
1	rPOD-A	101	FAM
2	ND-A	113	FAM
3	rPOD-B	117	FAM
4	CBR	132	FAM
5	ND-B	172	FAM
6	HLYA-A	105	HEX
7	AER-A	111	HEX
8	UPA	166	HEX
9	AER-B	175	HEX
10	HLYA-B	190	HEX
11	AER-C	213	HEX
12	HLYA-C	368	HEX

组织	MD-VF-Auto SEM法	浮游生物基因复合扩增体系
总阳性率	50/50（100%）	42/50（84%）^1）
肺	50/50（100%）	46/50（92%）
肝	48/50（96%）	39/50（78%）^1）
肾	49/50（98%）	38/50（76%）^1）

组织	MD-VF-Auto SEM法	浮游生物基因复合扩增体系
总阳性率	50/50（100%）	42/50（84%）^1）
肺	50/50（100%）	46/50（92%）
肝	48/50（96%）	39/50（78%）^1）
肾	49/50（98%）	38/50（76%）^1）

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