Changes of 1，5-AG in Vitreous Humor of Rabbit Cadavers with Hyperglycemic Metabolism

doi:10.12116/j.issn.1004-5619.2021.410403

Abstract

Abstract:

Objective To investigate the concentration and change characteristics of 1，5-anhydroglucitol （1，5-AG） in the vitreous humor of rabbit cadavers with hyperglycemic metabolism, and to explore the value of 1，5-AG in forensic pathology identification of death caused by hyperglycemic metabolism disorders. Methods A diabetic hyperglycemic rabbit model was established by using alloxan. Eighteen rabbits with fasting glucose concentration ≥13.80 mmol/L （experimental group） and 18 healthy rabbits with fasting glucose concentration ≤6.10 mmol/L （control group） were selected. After death from air embolism. The blood samples were collected immediately, and vitreous humor samples were collected at 0 h, 12 h, 24 h and 36 h after death. The concentration of 1，5-AG in the blood and vitreous humor of rabbits was determined. Results The blood glucose concentration in the experimental group was （25.10±3.14） mmol/L. At the time of death, there was no significant difference in the concentration of 1，5-AG in the blood [（0.94±0.20） μg/mL] and in the vitreous humor （0.99±0.05 μg/mL, P>0.05）. The concentration of 1，5-AG in the vitreous humor of the experimental group was lower than that of the corresponding control group at all time points （P<0.05）, and there was no significant difference betwwen 1，5-AG concentration in vitreous humor between earch time point in the experimental group and the control group （P>0.05）. Correlation analysis showed that the concentration of 1,5-AG in blood was negatively correlated with blood glucose in both control group and experimental group （control group: r=-0.79, P<0.05; experimental group: r=-0.97, P<0.05）. Conclusion Vitreous humor can replace blood as an effective test sample for 1,5-AG detection. The concentration of 1，5-AG in rabbit vitreous humor remains stable within 36 hours after death and is not affected by the change of postmortem interval. If the concentration of 1，5-AG decreases significantly, it indicates the existence of hyperglycemia in rabbits before death.

Key words: forensic pathology, 1，5-anhydroglucitol, hyperglycemic, vitreous humor, diabetes mellitus, rabbits

CLC Number:

DF795.1

Fang-Fang LIU, Hui WU, Wei WANG, Ying XIE. Changes of 1，5-AG in Vitreous Humor of Rabbit Cadavers with Hyperglycemic Metabolism[J]. Journal of Forensic Medicine, 2023, 39(1): 13-17.

Figures/Tables 3

References 22

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组别	左眼	右眼
对照	1.28±0.21	1.36±0.26
实验	0.88±0.07	0.91±0.08

组别	左眼	右眼
对照	1.28±0.21	1.36±0.26
实验	0.88±0.07	0.91±0.08

组别	0 h（n=6）	12 h（n=12）	24 h（n=12）	36 h（n=6）
对照	1.36±0.30	1.28±0.29	1.31±0.18	1.35±0.15
实验	0.99±0.05^1）	0.90±0.07^1）	0.92±0.08^1）	0.98±0.09^1）

组别	0 h（n=6）	12 h（n=12）	24 h（n=12）	36 h（n=6）
对照	1.36±0.30	1.28±0.29	1.31±0.18	1.35±0.15
实验	0.99±0.05^1）	0.90±0.07^1）	0.92±0.08^1）	0.98±0.09^1）

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