Research Progress on Molecular Changes in Pulmonary Hypoxia and Cause of Death Identification in Mechanical Asphyxia

doi:10.12116/j.issn.1004-5619.2022.421005

Abstract

Abstract:

Lung is the largest organ of the respiratory system. During hypoxia, pulmonary cells undergo rapid damage changes and activate the self-rescue pathways, thus leading to complex biomacromolecule modification. Death from mechanical asphyxia refers to death due to acute respiratory disorder caused by mechanical violence. Because of the absence of characteristic signs in corpse, the accurate identification of mechanical asphyxia has always been the difficulty in forensic pathology. This paper reviews the biomacromolecule changes under the pulmonary hypoxia condition and discusses the possibility of application of these changes to accurate identification of death from mechanical asphyxia, aiming to provide new ideas for related research.

Key words: forensic pathology, mechanical asphyxia, causes of death, hypoxia, lung, review

CLC Number:

DF795.1

Tian-pu WU, Jian-long MA, Xin-biao LIAO, Dong-chuan ZHANG, Kai-jun MA, Yan-geng YU, Long CHEN. Research Progress on Molecular Changes in Pulmonary Hypoxia and Cause of Death Identification in Mechanical Asphyxia[J]. Journal of Forensic Medicine, 2023, 39(1): 57-65.

Figures/Tables 1

References 66

1	OUIDDIR A， PLANÈS C， FERNANDES I， et al. Hypoxia upregulates activity and expression of the glucose transporter GLUT1 in alveolar epithelial cells[J]. Am J Respir Cell Mol Biol，1999，21（6）：710-718. doi：10.1165/ajrcmb.21.6.3751 .
2	GUO C J， ATOCHINA-VASSERMAN E N， ABRA-MOVA E， et al. S-nitrosylation of surfactant protein-D controls inflammatory function[J]. PLoS Biol，2008，6（11）：e266. doi：10.1371/journal.pbio.00 60266 .
3	AKELLA A， DESHPANDE S B. Pulmonary surfactants and their role in pathophysiology of lung disorders[J]. Indian J Exp Biol，2013，51（1）：5-22.
4	PHAM I， UCHIDA T， PLANES C， et al. Hypoxia upregulates VEGF expression in alveolar epithelial cells in vitro and in vivo [J]. Am J Physiol Lung Cell Mol Physiol，2002，283（5）： L1133-L1142. doi：10.1152/ajplung.00464.2001 .
5	吴坤兴，曾江海，李慧敏，等. 146例他杀尸检资料法医学分析[J].中国法医学杂志，2012，27（3）：241-242. doi：10.13618/j.issn.1001-5728.2012.03.011 .
	WU K X， ZENG J H， LI H M， et al. Forensic analysis of autopsy data of 146 cases of homicide[J]. Zhongguo Fayixue Zazhi，2012，27（3）：241-242.
6	MA J L， JING H J， ZENG Y， et al. Retrospective analysis of 319 hanging and strangulation cases between 2001 and 2014 in Shanghai[J]. J Forensic Leg Med，2016，42：19-24. doi：10.1016/j.jflm.2016.05.001 .
7	MAGHIN F， ANDREOLA S A， BORACCHI M， et al. Characterization with scanning electron microscopy/energy-dispersive X-ray spectrometry of microtraces from the ligature mean in hanging mechanical asphyxia： A series of forensic cases[J]. Am J Forensic Med Pathol，2018，39（1）：1-7. doi：10.1097/paf.0000000000000355 .
8	CHEN L， PAN H， MA J L， et al. An overhung mute suspect died during restraint - Is this a case of positional asphyxia? [J]. J Forensic Leg Med，2015，33：98-100. doi：10.1016/j.jflm.2015.04.002 .
9	ZENG Y， LV Y， TAO L， et al. G6PC3， ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers[J]. Oncotarget，2016，7（46）：74526-74536. doi：10.18632/oncotarget.12931 .
10	ZENG Y， TAO L， MA J， et al. DUSP1 and KCNJ2 mRNA upregulation can serve as a biomarker of mechanical asphyxia-induced death in cardiac tissue[J]. Int J Legal Med，2018，132（3）：655-665. doi：10.1007/s00414-017-1616-4 .
11	ZHANG H， HU Y， WANG H， et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death[J]. Am J Transl Res，2019，11（7）：4568-4583.
12	HAN L J， ZHANG H， ZENG Y， et al. Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia[J]. Forensic Sci Int，2020，311：110293. doi：10.1016/j.forsciint.2020.110293 .
13	HU Y， TIAN L， MA K， et al. ER stress-related protein， CHOP， may serve as a biomarker of mechanical asphyxia： A primary study[J]. Int J Legal Med，2022，136（4）：1091-1104. doi：10.1007/s00414-021-02770-1 .
14	FENG X， ZHANG D， GONG Q， et al. Expression of glucose-regulated protein 78 and miR-199a in rat brain after fatal ligature strangulation[J]. Am J Forensic Med Pathol，2017，38（1）：78-82. doi：10.1097/paf.0000000000000298 .
15	李继承，曾园山. 组织学与胚胎学[M]. 9版. 北京：人民卫生出版社，2018：159-161.
	LI J C， ZENG Y S. Histology and embryology[M]. 9th ed. Beijing： People’s Medical Publishing House， 2018：159-161.
16	施强强，刘莉，董年. 成纤维细胞生长因子10在肺损伤性修复中的研究进展[J].医学综述，2022，28（7）：1296-1301. doi：10.3969/j.issn.1006-2084.2022.07.009 .
	SHI Q Q， LIU L， DONG N. Research progress of fibroblast growth factor 10 in repair of lung injury[J]. Yixue Zongshu，2022，28（7）：1296-1301.
17	丁文龙. 系统解剖学[M]. 3版. 北京：人民卫生出版社，2018：135-136.
	DING W L. Systematic anatomy[M]. 3rd ed. Beijing： People’s Medical Publishing House，2018：135-136.
18	丛斌. 法医病理学[M].5版. 北京：人民卫生出版社，2016：276.
	CONG B. Forensic pathology[M]. 5th ed. Beijing： People’s Medical Publishing House，2016：276.
19	王建枝，钱睿哲. 病理生理学[M]. 9版. 北京：人民卫生出版社，2018：97-98.
	WANG J Z， QIAN R Z. Pathophysiology[M]. 9th ed. Beijing： People’s Medical Publishing House，2018：97-98.
20	LOENARZ C， COLEMAN M L， BOLEININGER A， et al. The hypoxia-inducible transcription factor pathway regulates oxygen sensing in the simplest animal， Trichoplax adhaerens[J]. EMBO Rep，2011，12（1）：63-70. doi：10.1038/embor.2010.170 .
21	COCKMAN M E， MASSON N， MOLE D R， et al. Hypoxia inducible factor-alpha binding and ubiquitylation by the von Hippel-Lindau tumor suppressor protein[J]. J Biol Chem，2000，275（33）：25733-25741. doi：10.1074/jbc.m002740200 .
22	DAMES S A， MARTINEZ-YAMOUT M， DE GUZMAN R N， et al. Structural basis for Hif-1α/CBP recognition in the cellular hypoxic response[J]. PNAS，2002，99（8）：5271-5276. doi：10.1073/pnas.082121399 .
23	CHILOV D， CAMENISCH G， KVIETIKOVA I， et al. Induction and nuclear translocation of hypoxia-inducible factor-1 （HIF-1）： Heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha[J]. J Cell Sci，1999，112（8）：1203-1212. doi：10.1242/jcs.112.8.1203 .
24	RIDDLE S R， AHMAD A， AHMAD S， et al. Hypoxia induces hexokinase Ⅱ gene expression in human lung cell line A549[J]. Am J Physiol Lung Cell Mol Physiol，2000，278（2）： L407-L416. doi：10.1152/ajplung.2000.278.2.l407 .
25	WANG S B， ZHANG H， XIA L X， et al. Circular RNA circ_0061140 accelerates hypoxia-induced glycolysis， migration， and invasion in lung adenocarcinoma through the microRNA-653/hexokinase 2 （HK₂） axis[J]. Bioengineered，2022，13（3）：7156-7166. doi：10.1080/21655979.2021.2000743 .
26	MATHEW T， SARADA S K S. Intonation of Nrf2 and Hif1-α pathway by curcumin prophylaxis： A potential strategy to augment survival signaling under hypoxia[J]. Respir Physiol Neurobiol，2018，258：12-24. doi：10.1016/j.resp.2018.09.008 .
27	MÜLLER H， SCHMIEDL A， WEISS C， et al. DMBT1 is upregulated in lung epithelial cells after hypoxia and changes surfactant ultrastructure[J]. Pediatr Pulmonol，2020，55（11）：2964-2969. doi：10.1002/ ppul.25018 .
28	KRICK S， EUL B G， HÄNZE J， et al. Role of hypoxia-inducible factor-1α in hypoxia-induced apoptosis of primary alveolar epithelial type Ⅱ cells[J]. Am J Respir Cell Mol Biol，2005，32（5）：395-403. doi：10.1165/rcmb.2004-0314oc .
29	OZAWA K， KONDO T， HORI O， et al. Expression of the oxygen-regulated protein ORP150 accelerates wound healing by modulating intracellular VEGF transport[J]. J Clin Invest，2001，108（1）：41-50. doi：10.1172/jci11772 .
30	BECK-SCHIMMER B， SCHIMMER R C， MADJD-POUR C， et al. Hypoxia mediates increased neutrophil and macrophage adhesiveness to alveolar epithelial cells[J]. Am J Respir Cell Mol Biol，2001，25（6）：780-787. doi：10.1165/ajrcmb.25.6.4433 .
31	VADÁSZ I， SZNAJDER J I. Gas exchange disturbances regulate alveolar fluid clearance during acute lung injury[J]. Front Immunol，2017，8：757. doi：10.3389/fimmu.2017.00757 .
32	BARTOSZEWSKA S， KAMYSZ W， JAKIELA B， et al. miR-200b downregulates CFTR during hypoxia in human lung epithelial cells[J]. Cell Mol Biol Lett，2017，22：23. doi：10.1186/s11658-017-0054-0 .
33	PARRA E， PÉREZGIL J. Composition， structure and mechanical properties define performance of pulmonary surfactant membranes and films[J]. Chem Phys Lipids，2015，185：153-175. doi：10.1016/j.chemphyslip.2014.09.002 .
34	MCCLINTOCK D S， SANTORE M T， LEE V Y， et al. Bcl-2 family members and functional electron transport chain regulate oxygen deprivation-induced cell death[J]. Mol Cell Biol，2002，22（1）：94-104. doi：10.1128/mcb.22.1.94-104.2002 .
35	SURESH M V， BALIJEPALLI S， ZHANG B Y， et al. Hypoxia-inducible factor （HIF）-1α promotes inflammation and injury following aspiration-induced lung injury in mice[J]. Shock，2019，52（6）：612-621. doi：10.1097/shk.0000000000001312 .
36	CLAUSS M， WEICH H， BREIER G， et al. The vascular endothelial growth factor receptor flt-1 mediates biological activities： Implications for a functional role of placenta growth factor in monocyte activation and chemotaxis[J]. J Biol Chem，1996，271（30）：17629-17634. doi：10.1074/jbc.271.30.17629 .
37	HASHIMOTO R， GUPTE S A. G6PD is a critical enabler of hypoxia-induced accumulation of macrophages and platelets in mice lungs and contributor to lung inflammation[J]. Vasc Pharmacol，2022，144：106976. doi：10.1016/j.vph.2022.106976 .
38	TANI N， IKEDA T， SHIDA A， et al. Postmortem water contents of major organs with regard to the cause of death[J]. J Forensic Leg Med，2019，65：48-54. doi：10.1016/j.jflm.2019.05.003 .
39	GILLE T， RANDRIANARISON-PELLAN N， GOO- LAERTS A， et al. Hypoxia-induced inhibition of epithelial Na⁺ channels in the lung. Role of Nedd4-2 and the ubiquitin-proteasome pathway[J]. Am J Respir Cell Mol Biol，2014，50（3）：526-537. doi：10.1165/rcmb.2012-0518oc .
40	GUSAROVA G A， DADA L A， KELLY A M， et al. Alpha1-AMP-activated protein kinase regulates hypoxia-induced Na， K-ATPase endocytosis via direct phosphorylation of protein kinase C zeta[J]. Mol Cell Biol，2009，29（13）：3455-3464. doi：10.1128/mcb.00054-09 .
41	MAGNANI N D， DADA L A， QUEISSER M A， et al. HIF and HOIL-1L-mediated PKCζ degradation stabilizes plasma membrane Na, K-ATPase to protect against hypoxia-induced lung injury[J]. Proc Natl Acad Sci U S A，2017，114（47）：E10178-E10186. doi：10.1073/pnas.1713563114 .
42	ZHANG Z Q， SONG Y L， CHEN Z H， et al. Deletion of aquaporin 5 aggravates acute lung injury induced by Pseudomonas aeruginosa[J]. J Trauma，2011，71（5）：1305-1311. doi：10.1097/ta.0b0 13e3182128528 .
43	NITTA C H， OSMOND D A， HERBERT L M， et al. Role of ASIC1 in the development of chronic hypoxia-induced pulmonary hypertension[J]. Am J Physiol Heart Circ Physiol，2014，306（1）：41-52. doi：10.1152/ajpheart.00269.2013 .
44	SUN H， PAUDEL O， SHAM J S K. Increased intracellular Cl^- concentration in pulmonary arterial myocytes is associated with chronic hypoxic pulmonary hypertension[J]. Am J Physiol Cell Physiol，2021，321（2）：C297-C307. doi：10.1152/ajpcell. 00172.2021 .
45	National Research Council （US） Committee on A Framework for Developing a New Taxonomy of Disease. Toward precision medicine： Building a knowledge network for biomedical research and a new taxonomy of disease[M]. Washington （DC）： National Academies Press （US），2011：21-40. doi：10.17226/13284 .
46	SCHAFFHAUSEN J. What precisely is precision medicine? [J]. Trends Pharmacol Sci，2017，38（1）：1-2. doi：10.1016/j.tips.2016.11.004 .
47	吕叶辉，李文灿，陈龙. 分子解剖技术在遗传性心律失常猝死鉴定中的应用[J].法医学杂志，2012，28（5）：362-365. doi：10.3969/j.issn.1004-5619.2012.05.010 .
	LÜ Y H， LI W C， CHEN L. Application of molecular autopsy in sudden death caused by inherited arrhythmia[J]. Fayixue Zazhi，2012，28（5）：362-365.
48	潘晖，吕叶辉，张恒，等. mRNA和microRNA在机械性窒息死推断中的研究进展[J].中国法医学杂志，2014，29（2）：121-123. doi：10.13618/j.issn.1001-5728.2014.02.009 .
	PAN H， LÜ Y H， ZHANG H， et al. Advances in applicability of mRNA and microRNA to estimate the cause of mechanical asphyxia death[J]. Zhongguo Fayixue Zazhi，2014，29（2）：121-123.
49	曾颜，马剑龙，陈龙. 缺氧相关microRNA在窒息死亡原因推断中的意义[J].法医学杂志，2017，33（1）：38-41. doi：10.3969/j.issn.1004-5619.2017.01.010 .
	ZENG Y， MA J L， CHEN L. Significance of hypoxia-related microRNA for estimating the cause of mechanical asphyxia death[J]. Fayixue Zazhi，2017，33（1）：38-41.
50	胡毅恺，张恒，肖碧，等. 线粒体缺氧性损伤及其法医学意义[J].法医学杂志，2020，36（2）：243-248. doi：10.12116/j.issn.1004-5619.2020.02.018 .
	HU Y K， ZHANG H， XIAO B， et al. Mitochondrial damage due to hypoxia and its forensic significance[J]. Fayixue Zazhi，2020，36（2）：243-248.
51	韩刘君，徐红梅，陈龙. 蛋白质组学及其在法医病理学中的应用[J].法医学杂志，2019，35（1）：78-83. doi：10.12116/j.issn.1004-5619.2019.01.015 .
	HAN L J， XU H M， CHEN L. Proteomics and its application in forensic pathology[J]. Fayixue Zazhi，2019，35（1）：78-83.
52	CHIGHINE A， LOCCI E， FERINO G， et al. Letter to the Editor regarding the article ‘ER stress-related protein， CHOP， may serve as a biomarker of mechanical asphyxia： A primary study’ by Hu and colleagues[J]. Int J Legal Med，2022，136（3）：969-970. doi：10.1007/s0044-022-02808-y .
53	HAN L J， LI W C， HU Y K， et al. Model for the prediction of mechanical asphyxia as the cause of death based on four biological indexes in human cardiac tissue[J]. Sci Justice，2021，61（3）：221-226. doi：10.1016/j.scijus.2021.02.003 .
54	CECCHI R， SESTILI C， PROSPERINI G， et al. Markers of mechanical asphyxia： Immunohistochemical study on autoptic lung tissues[J]. Int J Legal Med，2014，128（1）：117-125. doi：10.1007/s00414-013-0876-x .
55	SALAMA M， MOHAMED W M Y. Tau protein as a biomarker for asphyxia： A possible forensic tool?[J]. Appl Transl Genom，2016，9：20-22. doi：10.1016/j.atg.2016.03.001 .
56	GONZÁLEZ-HERRERA L， VALENZUELA A， RAMOS V， et al. Cardiac troponin T determination by a highly sensitive assay in postmortem serum and pericardial fluid[J]. Forensic Sci Med Pathol，2016，12（2）：181-188. doi：10.1007/s12024-016-9749-1 .
57	GONZÁLEZ-HERRERA L， MÁRQUEZ-RUIZ A B， SERRANO M J， et al. mRNA expression patterns in human myocardial tissue， pericardial fluid and blood， and its contribution to the diagnosis of cause of death[J]. Forensic Sci Int，2019，302：109876. doi：10.1016/j.forsciint.2019.109876 .
58	FERREIRA P G， MUÑOZ-AGUIRRE M， REVE-RTER F， et al. The effects of death and post-mortem cold ischemia on human tissue transcriptomes[J]. Nat Commun，2018，9（1）：490. doi：10.1038/s41467-017-02772-x .
59	MKRTCHIAN S， LEE K L， KÅHLIN J， et al. Hypoxia regulates microRNA expression in the human carotid body[J]. Exp Cell Res，2017，352（2）：412-419. doi：10.1016/j.yexcr.2017.02.027 .
60	TANI N， ISHIKAWA M， WATANABE M， et al. Thyroid-related hormones as potential markers of hypoxia/ischemia[J]. Hum Cell，2020，33（3）：545-558. doi：10.1007/s13577-020-00341-x .
61	MUCIACCIA B， SESTILI C， DE GROSSI S， et al. Are mast cells implicated in asphyxia? [J]. Int J Legal Med，2016，130（1）：153-161. doi：10.1007/s00414-015-1211-5 .
62	GUTJAHR E， MADEA B. Inflammatory reaction patterns of the lung as a response to alveolar hypoxia and their significance for the diagnosis of asphyxiation[J]. Forensic Sci Int，2019，297：315-325. doi：10.1016/j.forsciint.2019.02.026 .
63	王艳霞. 肺泡上皮细胞在低氧性肺动脉高压中的作用及机制研究[D].西安：中国人民解放军空军军医大学，2018.
	WANG Y X. Role and mechanism of alveolar epithelial cells in hypoxic pulmonary hypertension[D]. Xi’an： Air Force Medical University of the Chinese People’s Liberation Army，2018.
64	HUANG J， FRID M， GEWITZ M H， et al. Hypoxia-induced pulmonary hypertension and chronic lung di-sease： Caveolin-1 dysfunction an important underlying feature[J]. Pulm Circ，2019，9（1）：2045894019837876. doi：10.1177/2045894019837876 .
65	ALAM S EL， PENA E， AGUILERA D， et al. Inflammation in pulmonary hypertension and edema induced by hypobaric hypoxia exposure[J]. Int J Mol Sci，2022，23（20）：12656. doi：10.3390/ijms232012656 .
66	LI N， SHI Y， YIN J， et al. Impact of ATP synthase/coupling factor 6 in hypoxic pulmonary arterial hypertension： An experimental rat model[J]. Turk J Med Sci，2022，52（5）：1468-1477. doi：10. 55730/1300-0144.5485 .

涉及通路	蛋白质	缺氧下变化	细胞类型	参考文献
能量代谢相关蛋白	葡萄糖转运蛋白1	上调	肺泡上皮细胞	[1]
能量代谢相关蛋白	己糖激酶	上调	肺泡上皮细胞	[24-25]
肺泡表面蛋白	表面活性蛋白-B	下调	肺泡上皮细胞胞外基质	[26-27]
肺泡表面蛋白	表面活性蛋白-C、表面活性蛋白-D	上调	肺泡上皮细胞胞外基质	[2-3]
细胞凋亡相关蛋白	B细胞淋巴瘤-2家族蛋白	上调	原代ATⅡ	[28]
炎症反应相关分子	血管内皮生长因子	上调	肺泡上皮细胞游离面	[4]
	内质网氧调节伴侣蛋白150	上调	肺巨噬细胞	[29]
	细胞间黏附分子-1，血管细胞黏附分子-1	上调	肺泡上皮细胞	[30]
水盐调节相关蛋白	上皮钠通道	下调	肺泡上皮细胞	[31]
水盐调节相关蛋白	囊性纤维跨膜转导调节因子	下调	肺泡上皮细胞，支气管上皮细胞	[32]

涉及通路	蛋白质	缺氧下变化	细胞类型	参考文献
能量代谢相关蛋白	葡萄糖转运蛋白1	上调	肺泡上皮细胞	[1]
能量代谢相关蛋白	己糖激酶	上调	肺泡上皮细胞	[24-25]
肺泡表面蛋白	表面活性蛋白-B	下调	肺泡上皮细胞胞外基质	[26-27]
肺泡表面蛋白	表面活性蛋白-C、表面活性蛋白-D	上调	肺泡上皮细胞胞外基质	[2-3]
细胞凋亡相关蛋白	B细胞淋巴瘤-2家族蛋白	上调	原代ATⅡ	[28]
炎症反应相关分子	血管内皮生长因子	上调	肺泡上皮细胞游离面	[4]
	内质网氧调节伴侣蛋白150	上调	肺巨噬细胞	[29]
	细胞间黏附分子-1，血管细胞黏附分子-1	上调	肺泡上皮细胞	[30]
水盐调节相关蛋白	上皮钠通道	下调	肺泡上皮细胞	[31]
水盐调节相关蛋白	囊性纤维跨膜转导调节因子	下调	肺泡上皮细胞，支气管上皮细胞	[32]

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[13]	Xu-dong ZHANG, Yao-ru JIANG, Xin-rui LIANG, Tian TIAN, Qian-qian JIN, Xiao-hong ZHANG, Jie CAO, Qiu-xiang DU, Jun-hong SUN. Postmortem Interval Estimation Using Protein Chip Technology Combined with Multivariate Analysis Methods [J]. Journal of Forensic Medicine, 2023, 39(2): 115-120.
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