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28 April 2001, Volume 17 Issue 2 Previous Issue    Next Issue
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A study on the relationship between postmortem interval and the changes of DNA content in the kidney cellule of rat LIU LIANG;PENG DONG－ BIN;LIU YAN;ET AL.（ .DEPARTMENT OF FORENSIC MEDICINE TONGJI MEDICAL UNIVERSITY;WUHAN 000;.THE CRIMINAL SCIENCE RESEARCH INSTITUTION;BUREAU OF NANCHANG POLICE;NANCHANG 000） [ 2001, 0(2): 65-68.  Abstract ( 2079 )   Objective To study changes of DNA content in the kidney cellule of rats and relationship with the postmortem interval.Methods This experiment chose seven parameter of cell nuclear,including the area and integral optical density,determined the changes of DNA content in the kidney cellule of 15 rats at different intervals between 0 and 48h postmortem with auto－ TV－ image system.Results The degradation rate of DNA in nuclear has a certainty relationship to early PMI(in 48h) of rat,and get binomial regress equation. Conclusion Determining the quantity of DNA in nuclear should be an objective and exact way to estimate the PMI. [ Related Articles | Metrics

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A study on pathological changes of closed diffuse brain injury in rats with H.E.,esterification－ silver stain and albumin immunohistochemical stain YU XIAO－ JUN;XIAO FEI;WU JIA－ WEN;ET AL.(.DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE OF BEIHUA UNIVERSITY;JILIN 00;. COLLEGE OF FORENSIC MEDICINE;WEST CHINA UNIVERSITY OF MEDICAL SCIENCES;CHENGDU 00) [ 2001, 0(2): 69-73+7.  Abstract ( 1818 )   Objective To observed the pathological changes of closed diffuse brain injury in the rats died immediately and 15min to 5days after the injury. Methods H.E. staind and esterification－ silver stain were applied to investigate the closed diffuse brain injury.Results In rats died immediately after the concussive injury,a number of shrunken neurons(type I change),distended neurons(type II change)and wave－ like nerve fibers were identified in the brain tissue,especially in brain－ stem.At 2h and 8h after injury,brain edema and axonal swelling appeared clearly in the cortex and white matter,especially in brain－ stem.At post－ traumatic 8h and 24h,the axonal retraction balls began to appear.The amount of neurons undergoing type I and II changes and constraction balls increased along with the survivor time.After 4 days and 5 days,brain edema alleviated,but the retraction balls and axonal swelling still existed.With Esterifica－ tion silver stain,the above changes of neurons and nerve fibers were more obvious.With ABC stain,the distribution of albumin(Al) was extended from the perio－ vascular area to diffuse distribution.Al positive staining were more obvious in injuried neurons and nerve fibers.Conclusion The distribution of the concussive damage in the brain are coup,contra－ coup and centripental. [ Related Articles | Metrics

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Detection of Coxsackie virus B_3 gene in myocardium and spleen samples in myocarditis with sudden death CHEN YU－ CHUAN;CHENG JIAN－ DING;MA HUI－ HUI;ET AL.(.DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 000;.DEPARTMENT OF INFECTIOUS DISEASES;THIRD AFFILIATED HOSPITAL;SUN YAT－ SEN UNIVERSITY OF MEDICAL SC 2001, 0(2): 74-76.  Abstract ( 1928 )   Objective To detect the Coxsackie virus B_3(CVB_3) gene in myocardium and spleen tissues in viral myocarditis(VMC) with sudden death and to explore the diagnostic method for VMC by means of seeking pathogene.Methods By in situ RT－ PCR,the detection of CVB_3 gene in myocardium and spleen sections were performed in sudden death group caused by VMC and non－ cardiac death group.Results In VMC group,CVB_3 gene－ positive signals were seen in myocardium sections(3 out of total 8 cases， No.1,4,7 cases)and spleen sections(4 out of total 8 cases,No.2,4,6,7 cases).In non－ cardiac death group,no positive signals were detected in both myocardium and spleen tissues.Conclusion Positive detection of CVB_3 gene in both myocardium and spleen maybe an important character of VMC and can improve the detecting pathogene in diagnosing VMC. [ Related Articles | Metrics

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Study on the relationship between postmortem interval and the change of absorbance in vitreous humor of rabbit after death GONG ZHI－ QIANG;XU XIAO－ MING;SUN YUE－ GANG;ET AL.(.DEPARTMENT OF FORENSIC MEDICINE;JIANGXI MEDICAL COLLEGE;NANCHANG 000;.THE CRIMINAL SCIENCE RESEARCH INSTITUTION;BUREAU OF NACHANG POLICE;NACHANG 000) [ 2001, 0(2): 77-78.  Abstract ( 1887 )   Objective To seek a exact method of estimating Postmortem interval (PMI). Methods This study was preformed to investigate the relationship between postmortem interval and absorbance in vitreous humor of rabbit after death.The absorbance in vitreous humor of 48 rabbits after death were investigated with Model 754 spetrophotometer in apt wavelength (420nm).Results There exists positive linear regression association between postmortem interval (Y ) and absorbance in vitreous humor (X )(r=0.983 27, P< 0.05 ),during rabbits after death 0 to 72 hours.The formula of linear regression is Y=453.30X＋ 0.75(Y=postmortem interval=PMI,X=absorbance in vitreous humor).Conclusion The absorbance in vitreous humor can be as reference indicator to estimate PMI within hour 72 . [ Related Articles | Metrics

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Study of the mechanism of cultured neuron injury mediated by nitric oxide during hypoxia and oxidative stress GUAN PENG;AI XIAO－ MAN;YU RU－ TONG;ET AL. (. COLLEGE OF FORENSIC MEDICINE;WEST CHINA UNIVERSITY OF MEDICAL SCIENCE;CHENGDU 00;. LABORATORY IN BEIJING HOSPITAL;BEIJING 000;.DEPARTMENT OF NEUROSURGERY IN FIRST HOSPITAL;WEST CHINA UNI 2001, 0(2): 79-81+8.  Abstract ( 2133 )   Objective To study the mechanisms of cultured neurons injury mediated by nitric oxide and free oxygen radicle during hypoxia and oxidative stress.Methods The cultured newborn rat neurons were treated with hypoxia, H_2O_2 and pretreated superoxide dismutase (SOD) respectively. We examined the content of NO, malonaldehyde (MDA), lactate dehydrogenase (LDH) and SOD in cultured supernatant.Results Comparing with that of control group, the content of NO, LDH, MDA increased and the content of SOD decreased in hypoxia group and H_2O_2 group. The content between NO and SOD showed the negative correlation. Administration of 200 U/ml SOD before oxidative stress could efficiently decrease the release of NO, LDH and MDA in neurons. The content of NO, LDH and MDA manifested in postive correlation in each group.Conclusion Hypoxia and oxidative stress increased NO production which strengthen neurons injury induced by free radicle. SOD played an important role in elimination of free oxygen radicals and protecting neurons from injury by NO. [ Related Articles | Metrics

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Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application LI YUE;WANG SUI－ BAO;LIU CHAO;ET AL. (GUANGZHOU CRIMINAL SCIENCE＆ TECHNOLOGY INSTITUTE;GUANGZHOU 000) [ 2001, 0(2): 82-85.  Abstract ( 2072 )   Objective The genetic studies of 13 short tandem repeats(STRs) loci in two multiplex amplification systems were carried out on Chinese Han population in Guangdong . Methods DNA samples from 328 unrelated individuals were screened. The 13 loci were D3S1358、 vWA、 FGA、 D8S1179、 D21S11、 D18S51、 D5S818、 D13S317、 D7S820、 D16S539、 TH01、 TPOX and CSF1PO.The PCR products were analyzed and genotyped by ABI 377－ 96 Sequencer. Results The combined power of discrimination (DP) was 0.999 999 999 999 993 and the combined paternity of exclusion(PE) was 99.999％ . These 13 loci met the Hardy－ Weinberg expectations. Conclusion The two multiplex amplification systems were very useful in forensic case investigation. [ Related Articles | Metrics

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Study on application of CODIS loci to excluding paternity FANG JIAN－ XIN;LIU XI－ JIE;CHENG DA－ LIN (.INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000;.THE PEOPLE′ S PROCURATORCATE OF QUANZHOU;QUANZHOU 000) [ 2001, 0(2): 86-88.  Abstract ( 1835 )   Objective The study was carried out on application of CODIS loci (FGA, vWA, CSF1PO, TH01,TPOX, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11) in 100 cases of excluding paternity. Methods The PCR amplified products of Profiler Plus and Cofiler amplification kit were injected into a capillary on the ABI PRISM 310 Genetic Analyzer. GeneScan software analyzed the collected data, which can then be imported into Genotyper software for genotyping of alleles.Results In the group of Mother－ Child－ Alleged Father, more than 3 STR loci incompatibilities between alleged father and child were found in all observed cases, the mean incompatibility was 6.63 ; In the group of Alleged Father－ Child, 94.0％ of all cases observed was found more than 3 STR loci incompatibilities, the mean incompatibility was 5.01.Conclusion The results showed that CODIS loci had good application to excluding paternity, and the choice of hyperpolymorphic markers in investigation, which were valued by DP, H and PE, had direct relation with the augmentation of incompatibilities in excluding paternity. [ Related Articles | Metrics

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Chinese hans population study of fluorescent iabelled 8 STR multiplex system JIANG XIAN－ HUA;LI JUN;YU JIAO;ET AL.( LIAONING CRIMINAL SCIENCE＆ TECHNOLOGY INSTITUE;SHENYANG 00) [ 2001, 0(2): 89-92.  Abstract ( 2011 )   Objective Chinese Hans population study of 8 STR loci was carried out in 350 unrelated individuals. Methods the 8 STR loci(vWA,TH01,TPOX,CSF1PO,D5S818、 D13S317、 D7S820、 D16S539) and a sexual distinction locus Amelogenin, were amplified simultaneously in one PCR reaction tube, the products were separated by electrophoresis with PE－ 377 DNA sequencer, and analyzed by Genescan analysis software, then the accurate PCR fragment length and designation of genotypes were finished automatically by the sequencer. Results 63 alleles and 2 sex determination alleles were found in Hans population altogether; the minimum allele frequency in Chinese Hans population at these loci was 0.17％ , the maximum was 50.17％ ; the fragments were 119－ 327 base pairs long; the TDP of this system reached 99.999 999 98％ ; this system inherited steadily, and was accord with Mandel inheritance rule, no gene mutation was found, all loci met Hardy－ Weinberg equilibrium, but the frequencies of Hans population differed from those of African－ Americans, Caucasion－ Americans and Hispanic－ Americans. Conclusion this method is good for identification of little amount and degraded DNA samples from blood stain, semen stain mixed with vaginal excretion, hair root, cigarette root, muscle tissue and ancient bone. It can also make a foundation of establishing the National DNA Database. [ Related Articles | Metrics

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Recent progress of study on single nucleotide polymorphisms HUANG DAI－ XIN;ZHANG LIN;WU MEI－ YUN (COLLEGE OF FORENSIC MEDICINE;WEST CHINA UNIVERSITY OF MEDICAL SCIENCES;CHENGDU 00) [ 2001, 0(2): 122-125.  Abstract ( 1617 )   Single nucleotide polymorphisms (SNPs) is a new genetic marker system following RFLP and microsatellite polymorphism. It has been shown to have the characteristics of high－ density, inheritance stability and facilitation in analysis automation. SNPs can be detected by electrophoresis, endonuclease－ cleavage, PCR and sequencing, and can be used extensively in gene mapping, disease－ correlativity analysis, population genetics and drug research. [ Related Articles | Metrics