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28 June 2001, Volume 17 Issue 3 Previous Issue    Next Issue
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Study on the relationship between PMI and the concentration of zincum and nickel in the vitreous humor of rabbit after death GONG ZHI－ QIANG;XU XIAO－ MING;ZENG XIAN－ BIN;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;JIANGXI MEDICAL COLLEGE;NANCHANG 000;PEOPLE'S COURT OF NANCHANG;NANCHANG 000) 2001, 0(3): 129-131.  Abstract ( 1810 )   Objective To seek a exact method of estimating postmortem interval (PMI). Methods This study detected the concentration of zincum(Zn) and nickel(Ni) in vitreous humor of rabbit at hour 96 after death and explored the relationship between their concentration and PMI using a method ICP－ MS. Results The concentration of Zn and Ni in vitreous humor of rabbit at hour 24 after death were related to PMI significantly; The formulae of the relationship between PMI and Zn concentrations is y=0.140 4x2－ 1.335 1x＋ 3.829 8（ within 24h; R2=0.920 2） .The formula of the relationship between PMI and Ni concentrations is y=0.004 3x2－ 0.059 6x＋ 0.266 5（ within 24h; R2=0.910 3）。 Conclusion The concetration of Zn and Ni in vitreous humor of rabbit may be a reference indicator to estinmate early PMI. Related Articles | Metrics

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A study of the relation between interval of the injury and the reaction of neurons, glial cells after experimental brain contusion SONG LIAN－ FANG;MENG FAN TANG;LIU XI YU;ET AL. (.DEPARTMENT OF TECHNOLOGY;SUPER PEOPLE′ S COURT OF SHANDONG PROVINCE;JINAN;00;.INTERMEDIATE PEOPLE′ S COURT OF RIZHAO;SHANDONG PROVINCE;RIZHAO 00) 2001, 0(3): 132-136.  Abstract ( 1750 )   Objective To observe the change of c－ fos protein(Fos) and nerve growth factor receptor(NGFR) staining in the brain of rat after experimental brain contusion. Methods Immunohistochemistry of c fos and NGFR were applied to investigate the brain contusion. Results (1)The expression of Fos protein could be observed at 0.5h after injury and then increased with the prolonging of time.By 3h after injury,the positive staining cells could be detected massively not only in and round the wound site but also in other areas of the whole ipsilateral cortex.The stains decreased 6－ 12h later and could hardly be detected 1d after the brain contusion.The control－ experiment is negotive. (2)NGFR positive staining cells could be found round the wound area 1d postlesion. At 3d following injury,a peak of massive positively stained cells appeared both in number and in intensity, showing significant differences compaired with that of 1d after damage (P< 0.01). 5d later the positive express declined slowly.The express in the control－ rat is negotive. Conclusion There is a rule that the exprssion of Fos and NGFR positive staining changes with time going after brain contusion,which will be of great value in estimation of brain injury time. Detection of Fos can be used for time deduction in earlier period after injury,while NGFR in later period. They are also very important for distinguishing between antemortem or postmortem injury. Related Articles | Metrics

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Study on expression of the glycoprotein in myocardial cell membrane of the rat′s early myocardial ischemic LI ZHAO－ HUI;JING HUA－ LAN;WANG DU－ LUN. (DEPARTMENT OF FORENSIC MEDICINE;SUN YAT－ SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 00) 2001, 0(3): 137-138+.  Abstract ( 1892 )   Objective To explore expression of the glycoprotein in early myocardial ischemic .Methods The glycoprotein changes occured at the early acute cardiac ischemic area induced experimentally by ligation of left coronary artery of 32 SD rats. 6 lectins were measured by means of immunohistochemical metheds. Results Positive staining of PNA could be observed in ischemic area at 5min after ischemia, and the positive area increased with the prolongation of ischemic period. It became the strongest for 2h and then decreased . Conclusion This experiment proved that myocardial cell membrane in ischemia expressed D－ galactose.This may be of some value in forensic medicine practice. Related Articles | Metrics

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Histological study of species identification using bones ZHANG JI－ ZONG (DEPARTMENT OF FORENSIC MEDICINE;INSTITUTE OF FORENSIC SCIENCES;BEIJING 000) 2001, 0(3): 139-141.  Abstract ( 1966 )   Objective To explore the species identification methods of bone remain. Methods The compare between human bone and common animals bones was made with microscopic comparison methods. The sample was consisted of 77 slides including human,cattle, house, pig, goat, sheep, monkey, cat, rabbit, chicken, duck, pigeon, tortoise, frog, fish.Results The differences of the histological bone species were as follow: the thick of the outer and the inner circumferentical lamellae, and the shape of interstital lamellae, and the size of Haversian system, and the number of the circumferential lamellae, and the number of the lacunae of inner Haversian system. Conclusion The histological characters of the bones can be used for species identification. Related Articles | Metrics

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Ultrastructure changes of electrical injury in rats QIN ZHI－ QIANG;GONG YU－ CHANG;HUANG XIAO－ HUA (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000) 2001, 0(3): 142-144.  Abstract ( 1977 )   Objective To observe ultrastructure changes of electrical injury in rats. Methods An experimental model of rats suffered from the low voltage were designed. Ultrastructure changes of electrical injured tissues were observed under transmission electron microscope. Results (1)Plasm of epithelium was concreted in the affected areas and inner membrane system was broken. (2)Hypercontraction bands were observed in skeleton muscles. (3) There was dissolved necrosis and hypercontraction bands in the myocardieum. (4)Vacuoles were found in plasm of endothelium of blood vessels on electrical current path, and myelin sheath of nerve fiber were loosed. Conclusion The above mentioned ultrastructure changes could be used as assistant diagnositic index of electrocution. The mechanism of the changes were discussed. Related Articles | Metrics

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Research of ultra-structural pathological changes of nervous,endocrine and immune system in heroin addicts LI LI－HUA;YAO HONG;ZHAO YONG－HE;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;KUNMING MEDICAL COLLEGE;KUNMING 00) 2001, 0(3): 145-147+.  Abstract ( 2091 )   Objective To investigate ultrastructural pathological changes of Heroin－ Addicts.Methods Heroin－Addicts' central nervous system, endocrine system,immune system and reproductive system in 4 cases are observed by using transmission electron microscope(TEM).Results The changes of central nervous system are mitochondrion swelling, crista fragmentation and disappear. Endoplasmic reticulum dilation ,nervous fibres and cell organelles reduction ; mitochondrion swelling, Partial crista fragmentation and endoplasmic reticulum dilation are also found in endocrine system; Lymphocytes reduction, cytoplasm ingredient reduction and dead lymphocytes increase in immune system; in reproductive system, spermatogenic cells and cell organelles are reduced in the male and follicle disappeared in the female. Conclusion Ultra－ structural pathological changes of heroin－ addicts are presented acute, chronic oxygen deficiency degeneration and necrosis. Related Articles | Metrics

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Study on the construction of standard D1S549 allelic ladder via molecular clonning and its genetic polymorphism in Chinese three populations ZHANG LIN;XIN JUN PING;CHEN GUO DI;ET AL.(COLLEGE OF FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00) 2001, 0(3): 148-151.  Abstract ( 1715 )   Objective To resolve the problem of the accuracy and standardization of STR－ PCR typing in forensic practice, we have designed a new method to produce standard D1S549 allelic ladder. Methods Eight different PCR amplified D1S549 allelic fragments were isolated from the gel, eluted into the distilled water and re－ amplified by PCR. The purified allelic fragments were then blunt－ end subcloned individually into the pUC plasmid vectors and transfected into competent E.coli DH5α TM cells.Results The sequencing results confirmed that the size and the construe of the inserts were correct. The recombinant plasmids DNA with 8 inserts were then used as templates for re－ amplification to generate D1S549 standard ladder, with which the genetic polymorphisms of D1S549 locus in Chinese Han population in chengdu, Hui population in Gansu and Wei population in Xinjiang were studied. Conclusion The results showed that the standard ladder made via this method is excellent, and D1S549 locus is robust for genetic research and forensic application. Related Articles | Metrics

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Study on the forensic application of ABO genotyping by PCR-RFLP WANG XIAO－LI;CHENG XING－ZHI;LIU CHAO;ET AL.(FORENSIC SCIENCE INSTITUTE OF ZHONGSHAN CITY;ZHONGSHAN 0) 2001, 0(3): 152-154.  Abstract ( 2049 )   Objective The study was carried out on the application of ABO genotyping by PCR－ RFLP to forensic purpose. Methods The DNA of samples were extracted by Chelex－ 100 and phenol/chloroform, the PCR products were separated by Native Horizontal PAGE, and detected by silver staining. Results The PCR amplified fragment length of ABO loci is from 175 to 210bp, the frequency distribution of six genotypes ranges from 0.025 0 to 0.430 0, hetrozygosity(H) and discriminating power(Dp)is 0.516 2 and 0.711 1. Conclusion This technique has a good application to the forensic samples such as blood, blood stain, semen stain, hair root , bone tissue and semen from vaginal swabs. Related Articles | Metrics

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A study on polymorphism of salivary esterase in personal identification FAN AI－YING;ZAN YU－XI;LIU HONG－JUN;ET AL. (XINXIANG MEDICAL COLLEGE;HENAN 00) 2001, 0(3): 155-156.  Abstract ( 2036 )   Objective To research the value of polymorphism of salivary esterase(Set)in paternity and personal identification. Methods Phenotype and genotype of human salivary esterase were detected in 114 liquid saliva samples from the Chinese population by disc electrophoresis and fast blue RR staining assay. Results The frequency of Set type was F 22.81％ ,FS 50.88％ ,S 26.31％ .The estimated gene frequency of Set F was 0.482 5 and SetS was 0.517 5.The PE was 0.187 5 and the DP was 0.619 9.Conclusion Polymorphism of salivary esterase (Set) was practical in paternity and personal identification. Related Articles | Metrics

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Study on time limit of ACP and sperm positive-detected in mixed spot YU JIA－SHU;QIN KAI－LIANG;GUAN KE－HU;ET AL.(JISHOU PUBLIC SECURITY BUREAU OF HUNAN PROVINCE;JISHOU 000) 2001, 0(3): 157-159.  Abstract ( 1944 )   Objective The study was carried out on time limit of ACP and sperm positive－ detected in mixed spot. Methods 600 vaginal swabs from living body after sexual intercourse were tested by routine and three－ blindness methods.Results The longest time limit of ACP which was positively detected is 255h, average time limit is 52h, 41.3％ of those samples was ACP－ detected in positive degree (＋＋ ). The longest time limit of sperm positively detected is 132h, average time limit is 29h. The number of sperms observed in 34.3％ of those samples with positive result, is from 6 to 10/HP. Conclusion This study is helpful to forensic identification of sex cases. Related Articles | Metrics

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Advence on the schizoherenia of neuropathology LIU SHUI－PING;GUO WEI;CHEN YU－CHUAN (DEPARTMEUT OF FORENSIC PATHOLOGY;SUN YAT－ SEN UNIVERSITY OF MADICAL SCIENCES;GUANGZHOU 000) 2001, 0(3): 186-188.  Abstract ( 1991 )   This review summarizes the present state of knowledge, including the issues of structural imaging in schizophrenia, hemispheric asymmetry, neuro－ development and an integration of structure with function. Related Articles | Metrics

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Sex identification in forensic medicine WU WEN－QING;JIN YING (FU DAN MEDICAL UNIVERSITY;SHANGHAI 000) 2001, 0(3): 189-192.  Abstract ( 1928 )   The methods of morphology, cytology, sex hormone detection, DNA recobination and polymerase chain reaction (PCR) were described for sex identification. It was summarized that the morphology is better for skeleton identification; the amelogenin detection is most suggestible for the human tissues from which, enough quantity of genomic DNA is available. While sex chromatin examination gives only lower positive rates. The examination of sex hormone only be used for bloodstain sample.Because of the requirement of complex instruments and radio－ labeled probe, DNA molecular hybridization is now rarely used. Related Articles | Metrics