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28 August 2001, Volume 17 Issue 4 Previous Issue    Next Issue
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In vivo distribution and expression after skin injury of fibronectin splicing variants CHEN YI-JIU;LIU NING-GUO;HUANG XIAO-HUA;ET AL (THE INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;SHANGHAI 000) 2001, 0(4): 193-195.  Abstract ( 2049 )   Objective In order to develop new criteria and to select positive controls for forensic medicine, experiments on in vivo distribution and expression after skin wound of fibronectin splicing variants, EⅢA and EⅢB were studied. Methods Rat tissues were homogenized to extract total RNA which was then amplified by RT-PCR technique. Results (1)EⅢA+ and EⅢB+ of normal skin were not expressed, whereas EⅢA+ after 18 hours' injury was expressed abundantly. No obvious charge of EⅢB+ was detected. The differences between results of using two methods of injury were not significant.(2)The distribution of fibronectin variants in different adult organs were in the following order: brain >heart >liver. Conclusion EⅢA+ may be used as a sensitive criterion for research of skin injury. Related Articles | Metrics

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Fos expression in rat skin after different incised wound healing interval LIU NING-GUO;ZHAO ZI-QIN;GU YUN-JU;ET AL. (. INSTITUTION OF FORENSIC SCIENCES OF MINISTRY OF JUSTICE;SHANGHAI 000; .DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000) 2001, 0(4): 196-197.  Abstract ( 2001 )   Objective To investigate the expression of c-fos in rats' skin during wound healing. Methods Immunohistochemistry was conducted on parafin section from incised wounding model of rat skin. Results Fos protein improved from the time of 10 min after wounding in the wound edge, then it reached peak at 3h. 24h after injury, the quantity of Fos expression had no difference with that of normal skin. Conclusion Fos is sensitive after wound, but should be used with other criteria in wounding interval estimation as it's unstediness. Related Articles | Metrics

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A preliminary study on the changes of expression of PDGF-β, PDGFR-β, TGF-β1, TGFR, bFGF and its relationship with the wound age in wound healing WANG HUI-JUN;RUAN HAI-GEN;HUANG GUANG-ZHAO (.DEPATMENT OF FORENSIC PATHOLOGY;FIRST MILITARY MEDICAL UNIVERSITY;GUANGZHOU 0;.WUHAN CRIMINAL SCIENCE & TECHNOLOGY INSTITUTE;WUHAN 00;.DEPATMENT OF FORENSIC MEDICINE;TONGJI MEDICAL UNIVERSI 2001, 0(4): 198-201+.  Abstract ( 1666 )   Objective To explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound .Methods Immunohistochemical and image-analysis methods were performed on vital skin wounds(after incision 0.5-168h am) and postmortem damage(after incision 0.5-6h pm).Results The expression of the cytokines PDGF-β,PDGFR-β, TGF-β1, and bFGF in the epithelial cells was already enhanced since 0.5h am after damage and their strongest expression reaction was seen at 24-96h am. In addition, the expression of PDGF-β, PDGFR-β, TGF-β1 and bFGF was also found in the macrophages and the fibroblasts of the granulation tissue, and the expression changes in the postmortem damage group showed that the skin tissue within 0.5-3h after incision showed immunohistochemical changes but weakly expression and 3h thereafter no any change was found. Conclusion The expression characteristics of the above mentioned cytokines in wound repair should be related to the wound age and it reminds therefore that they may be used as immunohistochemical criteria for accurate determining the wound age. Related Articles | Metrics

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Experimental study of restiffening of the rigor mortis WANG XIN;LI MEI;LIAO ZHI-GANG;ET AL.(WEST CHINA FORENSIC MEDICAL COLLEGE;SICHUAN UNIVERSITY;CHENGDU 00) 2001, 0(4): 202-204.  Abstract ( 2307 )   Objective To observe changes of the length of sarcomere of rat when restiffening. Methods We measured the length of sarcomere of quadriceps in 40 rats in different condition by scanning electron microscope. Results The length of sarcomere of rigor mortis without destory is obviously shorter than that of restiffening. Conclusion The length of sarcomere is negatively correlative to the intensity of rigor mortis. Measuring the length of sarcomere can determine the intensity of rigor mortis and provide evidence for estimation of time since death. Related Articles | Metrics

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The preliminary study on change of serum enzyme level in crush syndrome rat LIU SHUI-PING;CHEN YU-CHUAN;GUO WEI;ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 000) 2001, 0(4): 205-206.  Abstract ( 1880 )   Objective To study the changes of serum creatinine kinase(CK) and its cardiac-specific isoenzyme compound(CK-MB) levels in crush injury rats.Methods Crush injury was produced in SD rats,the serum levels of CK and CK-MB were studied by automated biochemical analyzer.Results The levels of plasma CK and CK-MB were much higher in crush injury rats than those of the control group.Conclusions Cardiomyocyte injury may be induced in the early stage of crush injury rats. Related Articles | Metrics

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Diagnoisis of non-typical myocarditis by applying immunohistochemical method of CD_(68) LIN SHAO-YING;YAO QING-SONG;LIN JUN-LIAN;ET AL.(.DEPT OF PATHOLOGY;HAINAN MEDICAL COLLEGE;HAIKOU 00;. GUANGZHOU CRIMINAL SCIENCE & TECHNOLOGY INSTITUTE;GUANGZHOU 000) 2001, 0(4): 207-208+.  Abstract ( 2063 )   Objective To make a distinction between myocarditis and the reaction to some pathological state of myocardium. Methods Myocardiums of 26 cases with sudden cardiac death were stained and LM light microscopied with immunohistochemical method 10 cases with nomal myocardiums were contrasted. Results A great deal of stained positive monocyte of immunohistochemistry emerged in the parasetions of myocarditis patients with various farms and stacking(>15) . Conclusion The stain of immunohistochemistry can be used as one of the indications for diagnosing non-typical myocarditis. Related Articles | Metrics

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Effects of perming ,combing and stretching on hair keratins YAN PIN-HUA;QUE TING-ZHI;ZHAO ZHEN-MIN( INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.C SHANGHAI 000) 2001, 0(4): 209-211.  Abstract ( 2043 )   Objective Research of the hair damage due to perming, combing and strething can be of important volue for forensic hair individual identification. Methods The normal human hairs were treated with perming combing and stretching, and the keratins of the damage hair were analysed by using SDS-PAGGE and laser densimeter. Results Perming, combing and stretching brought about hair damage;The keratins of the damage hair were obviously reduced at the rang of molecular weight of 67 000～43 000 dalton. Conclusion The loss of the damage hair keratins were increased with the degree of the hair damage. Related Articles | Metrics

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The changes of tryptase activity in anaphylactic shock guinea-pigs GUO WEI;CHEN YU-CHUAN;LIU SHUI-PING;ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU;000 ) 2001, 0(4): 212-213.  Abstract ( 1971 )   Objective To investigate the changes of the activity of tryptase of sera, lungs and bronchial tubes in the guinea-pigs which suffered from hetero-serum anaphylactic shock. Methods Sera and tissues were collected from anaphylactic shock guinea-pigs,and the enzyme activity was tested colormetrically using special substrate,BAPNA.Results The activity of tryptase of sera,lungs and bronchial tubes increased significantly in Anaphylactic guinea-pigs compared with control group.Conclusion The changes of tryptase activity are helpful to diagnose anaphylactic shock. Related Articles | Metrics

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Study of distribution and metabolism of methamphetamine in hair of guinea pig JIANG YAN;SHEN MIN;ZHAO ZI-QIN;ET AL. (.DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000;.INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000) 2001, 0(4): 214-217.  Abstract ( 2145 )   Objective To study the distribution and metabolism of methamphetamine in the hair of guinea pig.Methods Determination of methamphetamine and its metabolite amphetamine in hair was performed by GC/MS and GC/NPD. Concentration-time course of methamphetamine and amphetamine in hair of guinea were recorded. Relationship between hair color, administrated dose and drug concentration in hair were also discussed. Results The concentration of amphetamine is higher than the concentration of methamphetamine in the hair of guinea administrated a single dose or seven doses of methamphetamine. The concentration of methamphetamine and amphetamine were significantly related with administration dose and the incorporation rate into white and brown hair is much poorer than that of black hair. Conclution Administration methods, dose and the color of hair affect the concentration of methamphetamine and amphetamine. Related Articles | Metrics

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The application of vascular endothelial growth factor in forensic science LI MEI;LIU MIN;WANG XIN;ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY;SI CHUAN UNIVERSITY;CHENGDU 00) 2001, 0(4): 239-241.  Abstract ( 1933 )   Vascular Endothelial Growth Factor (VEGF) is a potent angiogenic factor with a key role in several pathological processes, including wound repair as well as a effective vascular permeability factor. This article review the present study of VEGF in molecular biology, the connection with repair and expression regulation and so on. Related Articles | Metrics

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The research progress of the association of mitochondrial DNA mutation with cardiomyopathy LU JIAN-JU;LU HUI-LING (DEPARTMENT OF FORENSIC SEROLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES GUANGZHOU 00) 2001, 0(4): 242-244+.  Abstract ( 1805 )   There are some human diseases associated with mitochondrial DNA genome defect. Now many studies think that: oxygen radical resulting from oxidative phosphorylation(OXPHOS) disorder caused by myocardium ischemia and the increased OXPHOS induction damage mitochondrial DNA.Chronic damage accumulations lead to mitochondrial DNA deletion or point mutation in the end which show mitochondrial DNA 5.0kb or 7.4kb deletion and point mutation at position C15452A in the cytochrome b gene; the conservative sequence mutation of tRNA gene such as A4300G, C4320T point mutation in the tRNA Ilegene,A3243G point mutation in the tRNA legene etc result in defective contractile proteins whose persistent and inefficient contraction may increase the myocardium's metabolic demands for ATP and leads to cardiac hypertrophy. In this article, we review the study on the association of mitochondrial DNA mutation with ischemic cardiomyopathy and hypertrophic cardiomyopathy. Related Articles | Metrics

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The alternative splicing & expression of fibronectin Ⅲ_(CS) segment and its relationship with wound healing JI YAN;ZHAO ZI-QIN(DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000) 2001, 0(4): 245-248.  Abstract ( 2206 )   Fibronectin is an important large adhesive glycoprotein of the extracellular matrix, which is alternatively spliced in three regions, designated EⅢA,EⅢB and ⅢCS respectively. ⅢCS contains two binding domains for a variety of cell surface and extracellular ligands. Through this multiplicity of adhesive activities, ⅢCS can fulfill key roles in a broad spectrum of physiological processes, such as cell spreading and migration, differentiation and embryogenesis, wound healing, malignant transformation and metastasis, etc. Here, we will discuss the structure, biological property, and function of Ⅲcs splicing variants and its forensic applications. Related Articles | Metrics

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The single nucleotide polymorphisms and its application to forensic medicine HU RONG;WU XIN-YAO(DEPARTMENT OF FORENSIC MEDICINE;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 000) 2001, 0(4): 249-251+.  Abstract ( 1962 )   Single-nucleotide polymorphisms (SNPs) are the most abundant forms of human genetic variation. These variable sites are present at high density in the genome, making them powerful tool for the diagnosis of genentic and genentic-related diseases, population genetics research and drug development. They are also found widespread application to the forensic medicine. This report mainly describe the SNPs characteristics and its potential applications to the forensic medicine including the possibility, the problems and high-throughput automation detection methods. Related Articles | Metrics

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Imaging diagnosis and forensic appraisal of orbital fracture LIAO YI-LONG;QIN QI-SHENG;QIU QING-HUA ( PROCURATORATE OF CHIBI;HUBEI 00) 2001, 0(4): 252-254.  Abstract ( 1958 )   Misdiagnosis and missed diagnosis are common in forensic appraisal of orbital fracture. Now imaging technology is very important for studying the forensic features of orbital fracture and evaluating the degree of injury. This article reviews the classification, pathogenesis and imaging diagnosis of orbital fracture. It may do some help to forensic appraisal of orbital fracture. Related Articles | Metrics