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28 February 2002, Volume 18 Issue 1 Previous Issue    Next Issue
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Study on the change of bFGF in reticular formation of medulla oblongata after primary brain-stem injury XIA YU;XU XIAO-HU;WANG DENG-WEN;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;SHANTOU UNIVERSITY MEDICAL COLLEGE;SHANTOU;GUANGDONG 0) 2002, 0(1): 1-3.  Abstract ( 1960 )   Objective To study the effect of primary brain-stem injury on the expression of basic fibroblast growth factor (bFGF) in the reticular formation of medulla oblongata. Methods Immunohistochemical SABC was used to study the change of bFGF expression in the reticular formation of medulla oblongata after brain-stem injury by striking. Results The numbers of positive cells and positive intensity of the study group in the reticular formation of medulla oblongata were significantly elevated than those of the control group and the postmortem injury group . Conclusion The expression of bFGF is elevated in reticular formation after brain-stem injury. Related Articles | Metrics

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The expression of FN-Ⅲcs in the wound healing and its relationship with injury time JI YAN;ZHAO ZI-QIN;GU YUN-JU;ET AL.(DEPARTMENT OF FORENSIC MEDICINE OF FUDAN UNIVERSITY;SHANGHAI 000) 2002, 0(1): 4-6.  Abstract ( 1913 )   Objective To research the expression of alternative splicing segment of fibronectin-Ⅲcs in incised wound of skin, to offer the experimental data for early injury time judgement.Methods Using in situ hybridization with DIG labeled anti sense RNA probe, the expression of FN Ⅲcs domain was detected in both human and rat skin incised wound. Results (1) The expression of FN Ⅲcs improved from the time of 30 min after injury, reached peak at 6h and then reduced gradually.(2) The positive expression cells were mainly distributed in hair follicles, sebaceous glands and endothelium, however, in human beings they were basement cells of epidermis. Conclusion The expression of FN Ⅲcs domain would be a supposed useful criteria for early injury time judgement. Related Articles | Metrics

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The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe LIU NING-GUO;ZHAO ZI-QIN;GU YUN-JU;ET AL. (. INSTITUTION OF FORENSIC SCIENCES OF MINISTRY OF JUSTICE;SHANGHAI 000;.DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000) 2002, 0(1): 7-8+11.  Abstract ( 2106 )   Objective To estimate the effort between antisense RNA probe and random primed DNA probe. Methods The in situ hybridization was conducted on parafin section from wounding model of rat skin. Results Although both probes appeared positive staining, RNA probes was superior to DNA probes in terms of depth of staining and background. Conclusion RNA probe showed more satisfactorily on ISH. Related Articles | Metrics

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An immunohistochemical study on the distribution in organs in cases with morphine poisioning SHAN YA-MING;HAO CHUN-YU;WANG LONG;ET AL. (SHENYANG CRIMINAL SCIENCE AND TECHNOLOGY INSTITUTE;SHENYANG 000) 2002, 0(1): 9-11.  Abstract ( 2066 )   Objective To investigate the distribution of mophine in organs in cases with morphine poisioning to select ideal organs for immunohistochemical derection. Methods Localization and half quantitation of morphine in the brain, the kidney, the heart,and the liver were studied in 8 cases with morphine poisoning by immunohistochemical SP method. Results Morphine was mainly detected in the cytoplasm of certain parenchymal cells of the organs. The distribution varied greatly with different cases and organs. In the brain and kidney, morphine-positive cells could be easily found. Conclusion The kidney and brain may be the ideal organs for sampling in suspected morphine poisoning cases with. Related Articles | Metrics

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Immunohistochemical study with depletion of cardiac troponin T in rats on early myocardial ischaemia DENG DA-ZHONG;CHEN YU-CHUAN;HU BIN-JIE;ET AL. (.DEPARTMENT OF CRIMINAL TECHNIQUE;GUANGDONG POLICE COLLEGE;GUANGZHOU 0;.DEPARTMENT OF FORENSIC PATHOLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU 00) 2002, 0(1): 12-14+6.  Abstract ( 2107 )   Objective To study the depletion of cardiac troponin T in Rats of early myocardial ischaemia. Methods Immunohistochemical method (LSAB) was used and the depletion area of CTnT in myocardial ischaemia were measured by the computer image analysis. Results There is obviously depletion of CTnT after 15 min in myocardial ischaemia of rats, the mean of CTnT depletion of myocardial ischaemic group is significant difference compared with that of the control group(P< 0.01), and the depletion area increased with the prolongagtion of ischaemic time. Conclusion The depletion of CTnT is one of sensitive signs of early myocardial ischaemia. Related Articles | Metrics

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The experimental study of expression of intercellular adhesion molecule-1 after fluid percussion brain injury WANG XIN;LI MEI;LIAO ZHI-GANG;ET AL (DEPARTMENT OF FORENSIC PATHOLOGY;SICHUAN UNIVERSITY;WEST CHINA FORENSIC MEDICAL COLLEGE;CHENGDU;SICHUAN 00) 2002, 0(1): 15-16+1.  Abstract ( 1924 )   Objective To determine the changes of intercellular adhesion molecule-1 after traumatic brain injury. Methods Use the fluid-percussion model to inflict rat brain and take the samples at 2?4?8?12?24 hours after brain injury. The samples were studied by immunohistochemistry method combined with image analysis by MIAS image analysis system. Result The expression of ICAM-1 upregulates at 2 hours, reaches peak at 12 hours and falls down at 24 hours after brain injury. Conclusion The changes of ICAM-1 in brain can provide a backup for estimation of injury time and survival period after brain injury which sustained within 24 hours. Related Articles | Metrics

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A study of genetic polymorphism of the STR loci D20S85 and D6S477 in Han population living in Wuhan SONG HAI-YAN;YANG RONG-ZHI;YANG QING-EN;ET AL. (DEPARTMENT OF FORENSIC BIOLOGY;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN;HUBEI;000 ) 2002, 0(1): 17-18.  Abstract ( 1914 )   Objective The genetic polymorphism of two STR loci, D20S85 and D6S477, were studied in 280 unrelated Chinese individuals in Wuhan. Methods The PCR amplified products were analyzed by PAGE and silver staining. Results 10 and 9 alleles were observed in these two STR loci, and the discriminating power (DP) were 0.9085 and 0.9127 respectively. No deviations from Hardy-Weinberg equilibrium were found. The two STR loci had been successfully applied to individual identification and paternity testing. Conclusion The results demonstrated that the two loci were useful for forensic identification. Related Articles | Metrics

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Evaluation on the number and value of STR loci applied in paternity identification FANG JIAN-XIN;CHENG DA-LIN (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000) 2002, 0(1): 19-21.  Abstract ( 2156 )   Objective The study was carried out on the evaluation of number and value of STR loci applied in paternity identification. Methods A total of 13 STR loci, divided into four groups, was observed in 102 cases of paternity exclusion and 100 cases of paternity inclusion. PCR amplified products of 13 STR loci were injected into a capillary on the ABI PRISM 310 Genetic Analyzer. GeneScan software analyzed the collected data, which can then be imported into Genotyper software for genotyping of alleles. Results At least 3 STR loci incompatibilities between alleged father and child were found in all paternity exclusion cases of two observed groups which Cumulative Probability of Exclusion (CPE) was more than 99.99%, and in all paternity inclusion cases of those same observed groups, their Relative Chance of Paternity(RCP) could be over 99.99%.Conclusion The exclusion of paternity should be based on at least three STR loci incompatibilities in the identification practice. As a criterion for evaluation of the number and value of STR loci applied in paternity test,CPE should reach 99.99%. Related Articles | Metrics

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Screening of benzodizepines and their metabolites in urine SHEN BAO-HUA;SHEN MIN;ZHUO XIAN-YI;ET AL.( INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;SHANGHAI 000) 2002, 0(1): 22-25.  Abstract ( 2082 )   Objective Screen seventeen benzodizepines and their metabolitesin urine by GC/ECDand GC/MS . Methods They were GC (GC/ECD, GC/MS) assay of benzodizepines and GC (GC/ECD, GC/MS) assay of benzophenones of acid-hydrolytic products of 1,4-benzodizepines. Results The methods were simple and sensitive. The recoverys were 60%to 90%of most benzodizepines, linear calibration curves were 20ng/ml-200ng/ml(r >0.99), and detection limits were 0.5ng/ml-10ng/ml. Conclusion They methods were evaluated with human urine samples. Related Articles | Metrics

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Stress and hippocampus CAI WEI-XIONG;YANG DE-SEN (. INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P. R. CHINA;SHANGHAI;000;. DEPARTMENT OF MENTAL HEALTH;THE SECOND XIANGYA HOSPITAL;CENTRAL SOUTH UNIVERSITY;CHANGSHA;00) 2002, 0(1): 48-51.  Abstract ( 1902 )   This review summarizes the present advance of effects of stress on hippocampal structure and function and the role of hippocampus in feedback regulation of thalamic-pituitary-adrenocortical (HPA)axis during stress. It shows that stress can affect hippocampal structure and function, on the other hand,the hippocampus can also suppress the stress reaction through the feedback regulation of HPA axis,but chronic stress can attenuate this regulation, then significantly impair its structure and function. Related Articles | Metrics

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Injury by electrical forces: pathophysiology, biophysics and pathogenesis DONG LI;CHEN YU-CHUAN (DEPARTMENT OF FORESIC MEDICINE;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCE;GUANGZHOU 00) 2002, 0(1): 52-55+5.  Abstract ( 1713 )   As the technologic sophistication of generation and distribution of electrical energy has grown, so has the general concern about the effects of electric fields on human health. There can be no doubt that the significance of electrical trauma will continue to grow with our increasing use of power. It is apparent that our understanding of the various forms of electric trauma must increase, while we continue to promote safety near electrical hazards and develop effective medical therapies. Tissue damage as a result of electrical injury occurs by two mechanisms which are summative in action and have a variable degree of contribute to the ultimate damage produced. Thermal tissue damage occurs as a result of heat generated within the tissue (which offer an electrical resistance ) secondary to the passage of the electrical current. High temperatures can also lead to cell membrane components, e.g, phospholipids, to dissolve. Electroportation damage is the tissue damage induced secondary to the strong electric field. Transmembrane potentials caused by electrical current result in the formation of pore in the phospholipid component of the cell membrane resulting in loss of function of the cell membrane with consequent cell death. Related Articles | Metrics

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The applying and foreground of quantifying DNA content by image analysis technique in determining postmortem interval WANG CHENG-YI;LIU LIANG (DEPARTMENT OF FORENSIC PATHOLOGY;TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN;HUBEI 000) 2002, 0(1): 56-59.  Abstract ( 1873 )   Image Analysis Technique(IAT) was developed at 1950's, which quantifies the changing all the part of image by sampling, processing, quantifying ,computing, analyzing the information of image. And now it has become a normal quantifying technique in biology and medicine research. In the present paper, we reviewed briefly the principium of quantifying the DNA content by IAT, the law of degradation of DNA in nucleus and the foreground of this method in determining PMI in forensic pathology. Related Articles | Metrics