The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe

›› 2002, Vol. 0 ›› Issue (1): 7-8+11.

The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe

LIU NING-GUO1,ZHAO ZI-QIN2,GU YUN-JU2,ET AL. (1. INSTITUTION OF FORENSIC SCIENCES OF MINISTRY OF JUSTICE,SHANGHAI 200063;2.DEPARTMENT OF FORENSIC MEDICINE,MEDICAL CENTER OF FUDAN UNIVERSITY,SHANGHAI 200032)

Online:2002-02-25 Published:2002-02-28

Abstract

Abstract: Objective To estimate the effort between antisense RNA probe and random primed DNA probe. Methods The in situ hybridization was conducted on parafin section from wounding model of rat skin. Results Although both probes appeared positive staining, RNA probes was superior to DNA probes in terms of depth of staining and background. Conclusion RNA probe showed more satisfactorily on ISH.

Key words: in situ hybridization, digoxin, antisense RNA probe, random primed DNA probe

LIU NING-GUO;ZHAO ZI-QIN;GU YUN-JU;ET AL. (. INSTITUTION OF FORENSIC SCIENCES OF MINISTRY OF JUSTICE;SHANGHAI 000;.DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000). The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe[J]. , 2002, 0(1): 7-8+11.

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[2]	ZHOU JIAN-QING;WANG YONG-ZHONG;JIANG HONG-YAN;KONG JIE(.THE CRIMINAL TECHNIQUE INSTITUTE OF ZHENJIANG PUBLIC SECURITY BUREAU;ZHENJIANG 00;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE OF JIANGSU UNIVERSITY;ZHENJIANG 00;CHINA;. Expression of CGRP in Rat's Skin and Post-traumatic Interval [J]. , 2007, 0(3): 193-195+.
[3]	WU XU;WANG BAO-JIE;ZHANG GUO-HUA;ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY;SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG000;CHINA). The dynamically changes of COX-2and the gene expression of COX-2mRNA after traumatic brain injury in rats [J]. , 2004, 0(1): 4-6+8.
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[5]	MA MENG-YUN;XU XIAOHU (SHENZHEN CRIMINAL SCIENCE & TECHNOLOGY INSTITUTE;SHENZHEN 00;CHINA;COLLEGE OF MEDICAL SCIENCE;SHANTOU UNIVERSITY;SHANTOU 0;CHINA). Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats [J]. , 2003, 0(2): 65-67+1.
[6]	XIAO JUN-HUI;LI YONG-HONG(DEPARTMENT OF FORENSIC PATHOLOGY;WANNAN MEDICAL COLLEGE;WUHU00). The expression of HSP70mRNA after myocardial ischemia and reperfusion injury by in situ hybridization [J]. , 2002, 0(4): 199-200+.
[7]	JI YAN;ZHAO ZI-QIN;GU YUN-JU;ET AL.(DEPARTMENT OF FORENSIC MEDICINE OF FUDAN UNIVERSITY;SHANGHAI 000). The expression of FN-Ⅲcs in the wound healing and its relationship with injury time [J]. , 2002, 0(1): 4-6.
[8]	CHEN LONG;ET AL.( DEPARTMENT OF FORENSIC MEDICINE;MEDICAL CENTER OF FUDAN UNIVERSITY;SHANGHAI 000). Study on the expression of fibronectin after cerebral contusion in rats for timing of injuries [J]. , 2001, 0(1): 1-3+61.
[9]	LIU NINGGUO;ET AL ( SHANGHAI MEDICAL UNIVERSITY;SHANGHAI 000;P R CHINA). The expression of splicing fibronectin variants of cutaneous injuries in rats [J]. , 2000, 0(2): 70-71+7.

The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe

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